Eintrag weiter verarbeiten
Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models
Gespeichert in:
| Zeitschriftentitel: | Scientific Reports |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Scientific Reports, 7, 2017, 1 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Springer Science and Business Media LLC
|
| Schlagwörter: |
| author_facet |
Smits, Jos P. H. Niehues, Hanna Rikken, Gijs van Vlijmen-Willems, Ivonne M. J. J. van de Zande, Guillaume W. H. J. F. Zeeuwen, Patrick L. J. M. Schalkwijk, Joost van den Bogaard, Ellen H. Smits, Jos P. H. Niehues, Hanna Rikken, Gijs van Vlijmen-Willems, Ivonne M. J. J. van de Zande, Guillaume W. H. J. F. Zeeuwen, Patrick L. J. M. Schalkwijk, Joost van den Bogaard, Ellen H. |
|---|---|
| author |
Smits, Jos P. H. Niehues, Hanna Rikken, Gijs van Vlijmen-Willems, Ivonne M. J. J. van de Zande, Guillaume W. H. J. F. Zeeuwen, Patrick L. J. M. Schalkwijk, Joost van den Bogaard, Ellen H. |
| spellingShingle |
Smits, Jos P. H. Niehues, Hanna Rikken, Gijs van Vlijmen-Willems, Ivonne M. J. J. van de Zande, Guillaume W. H. J. F. Zeeuwen, Patrick L. J. M. Schalkwijk, Joost van den Bogaard, Ellen H. Scientific Reports Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models Multidisciplinary |
| author_sort |
smits, jos p. h. |
| spelling |
Smits, Jos P. H. Niehues, Hanna Rikken, Gijs van Vlijmen-Willems, Ivonne M. J. J. van de Zande, Guillaume W. H. J. F. Zeeuwen, Patrick L. J. M. Schalkwijk, Joost van den Bogaard, Ellen H. 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/s41598-017-12041-y <jats:title>Abstract</jats:title><jats:p>The strong societal urge to reduce the use of experimental animals, and the biological differences between rodent and human skin, have led to the development of alternative models for healthy and diseased human skin. However, the limited availability of primary keratinocytes to generate such models hampers large-scale implementation of skin models in biomedical, toxicological, and pharmaceutical research. Immortalized cell lines may overcome these issues, however, few immortalized human keratinocyte cell lines are available and most do not form a fully stratified epithelium. In this study we compared two immortalized keratinocyte cell lines (N/TERT1, N/TERT2G) to human primary keratinocytes based on epidermal differentiation, response to inflammatory mediators, and the development of normal and inflammatory human epidermal equivalents (HEEs). <jats:italic>Stratum corneum</jats:italic> permeability, epidermal morphology, and expression of epidermal differentiation and host defence genes and proteins in N/TERT-HEE cultures was similar to that of primary human keratinocytes. We successfully generated N/TERT-HEEs with psoriasis or atopic dermatitis features and validated these models for drug-screening purposes. We conclude that the N/TERT keratinocyte cell lines are useful substitutes for primary human keratinocytes thereby providing a biologically relevant, unlimited cell source for <jats:italic>in vitro</jats:italic> studies on epidermal biology, inflammatory skin disease pathogenesis and therapeutics.</jats:p> Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models Scientific Reports |
| doi_str_mv |
10.1038/s41598-017-12041-y |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9zNDE1OTgtMDE3LTEyMDQxLXk |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9zNDE1OTgtMDE3LTEyMDQxLXk |
| institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
Springer Science and Business Media LLC, 2017 |
| imprint_str_mv |
Springer Science and Business Media LLC, 2017 |
| issn |
2045-2322 |
| issn_str_mv |
2045-2322 |
| language |
English |
| mega_collection |
Springer Science and Business Media LLC (CrossRef) |
| match_str |
smits2017immortalizedntertkeratinocytesasanalternativecellsourcein3dhumanepidermalmodels |
| publishDateSort |
2017 |
| publisher |
Springer Science and Business Media LLC |
| recordtype |
ai |
| record_format |
ai |
| series |
Scientific Reports |
| source_id |
49 |
| title |
Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_unstemmed |
Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_full |
Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_fullStr |
Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_full_unstemmed |
Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_short |
Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_sort |
immortalized n/tert keratinocytes as an alternative cell source in 3d human epidermal models |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1038/s41598-017-12041-y |
| publishDate |
2017 |
| physical |
|
| description |
<jats:title>Abstract</jats:title><jats:p>The strong societal urge to reduce the use of experimental animals, and the biological differences between rodent and human skin, have led to the development of alternative models for healthy and diseased human skin. However, the limited availability of primary keratinocytes to generate such models hampers large-scale implementation of skin models in biomedical, toxicological, and pharmaceutical research. Immortalized cell lines may overcome these issues, however, few immortalized human keratinocyte cell lines are available and most do not form a fully stratified epithelium. In this study we compared two immortalized keratinocyte cell lines (N/TERT1, N/TERT2G) to human primary keratinocytes based on epidermal differentiation, response to inflammatory mediators, and the development of normal and inflammatory human epidermal equivalents (HEEs). <jats:italic>Stratum corneum</jats:italic> permeability, epidermal morphology, and expression of epidermal differentiation and host defence genes and proteins in N/TERT-HEE cultures was similar to that of primary human keratinocytes. We successfully generated N/TERT-HEEs with psoriasis or atopic dermatitis features and validated these models for drug-screening purposes. We conclude that the N/TERT keratinocyte cell lines are useful substitutes for primary human keratinocytes thereby providing a biologically relevant, unlimited cell source for <jats:italic>in vitro</jats:italic> studies on epidermal biology, inflammatory skin disease pathogenesis and therapeutics.</jats:p> |
| container_issue |
1 |
| container_start_page |
0 |
| container_title |
Scientific Reports |
| container_volume |
7 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792347086088306698 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:49:24.895Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Immortalized+N%2FTERT+keratinocytes+as+an+alternative+cell+source+in+3D+human+epidermal+models&rft.date=2017-09-19&genre=article&issn=2045-2322&volume=7&issue=1&jtitle=Scientific+Reports&atitle=Immortalized+N%2FTERT+keratinocytes+as+an+alternative+cell+source+in+3D+human+epidermal+models&aulast=van+den+Bogaard&aufirst=Ellen+H.&rft_id=info%3Adoi%2F10.1038%2Fs41598-017-12041-y&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792347086088306698 |
| author | Smits, Jos P. H., Niehues, Hanna, Rikken, Gijs, van Vlijmen-Willems, Ivonne M. J. J., van de Zande, Guillaume W. H. J. F., Zeeuwen, Patrick L. J. M., Schalkwijk, Joost, van den Bogaard, Ellen H. |
| author_facet | Smits, Jos P. H., Niehues, Hanna, Rikken, Gijs, van Vlijmen-Willems, Ivonne M. J. J., van de Zande, Guillaume W. H. J. F., Zeeuwen, Patrick L. J. M., Schalkwijk, Joost, van den Bogaard, Ellen H., Smits, Jos P. H., Niehues, Hanna, Rikken, Gijs, van Vlijmen-Willems, Ivonne M. J. J., van de Zande, Guillaume W. H. J. F., Zeeuwen, Patrick L. J. M., Schalkwijk, Joost, van den Bogaard, Ellen H. |
| author_sort | smits, jos p. h. |
| container_issue | 1 |
| container_start_page | 0 |
| container_title | Scientific Reports |
| container_volume | 7 |
| description | <jats:title>Abstract</jats:title><jats:p>The strong societal urge to reduce the use of experimental animals, and the biological differences between rodent and human skin, have led to the development of alternative models for healthy and diseased human skin. However, the limited availability of primary keratinocytes to generate such models hampers large-scale implementation of skin models in biomedical, toxicological, and pharmaceutical research. Immortalized cell lines may overcome these issues, however, few immortalized human keratinocyte cell lines are available and most do not form a fully stratified epithelium. In this study we compared two immortalized keratinocyte cell lines (N/TERT1, N/TERT2G) to human primary keratinocytes based on epidermal differentiation, response to inflammatory mediators, and the development of normal and inflammatory human epidermal equivalents (HEEs). <jats:italic>Stratum corneum</jats:italic> permeability, epidermal morphology, and expression of epidermal differentiation and host defence genes and proteins in N/TERT-HEE cultures was similar to that of primary human keratinocytes. We successfully generated N/TERT-HEEs with psoriasis or atopic dermatitis features and validated these models for drug-screening purposes. We conclude that the N/TERT keratinocyte cell lines are useful substitutes for primary human keratinocytes thereby providing a biologically relevant, unlimited cell source for <jats:italic>in vitro</jats:italic> studies on epidermal biology, inflammatory skin disease pathogenesis and therapeutics.</jats:p> |
| doi_str_mv | 10.1038/s41598-017-12041-y |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9zNDE1OTgtMDE3LTEyMDQxLXk |
| imprint | Springer Science and Business Media LLC, 2017 |
| imprint_str_mv | Springer Science and Business Media LLC, 2017 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 2045-2322 |
| issn_str_mv | 2045-2322 |
| language | English |
| last_indexed | 2024-03-01T17:49:24.895Z |
| match_str | smits2017immortalizedntertkeratinocytesasanalternativecellsourcein3dhumanepidermalmodels |
| mega_collection | Springer Science and Business Media LLC (CrossRef) |
| physical | |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | Springer Science and Business Media LLC |
| record_format | ai |
| recordtype | ai |
| series | Scientific Reports |
| source_id | 49 |
| spelling | Smits, Jos P. H. Niehues, Hanna Rikken, Gijs van Vlijmen-Willems, Ivonne M. J. J. van de Zande, Guillaume W. H. J. F. Zeeuwen, Patrick L. J. M. Schalkwijk, Joost van den Bogaard, Ellen H. 2045-2322 Springer Science and Business Media LLC Multidisciplinary http://dx.doi.org/10.1038/s41598-017-12041-y <jats:title>Abstract</jats:title><jats:p>The strong societal urge to reduce the use of experimental animals, and the biological differences between rodent and human skin, have led to the development of alternative models for healthy and diseased human skin. However, the limited availability of primary keratinocytes to generate such models hampers large-scale implementation of skin models in biomedical, toxicological, and pharmaceutical research. Immortalized cell lines may overcome these issues, however, few immortalized human keratinocyte cell lines are available and most do not form a fully stratified epithelium. In this study we compared two immortalized keratinocyte cell lines (N/TERT1, N/TERT2G) to human primary keratinocytes based on epidermal differentiation, response to inflammatory mediators, and the development of normal and inflammatory human epidermal equivalents (HEEs). <jats:italic>Stratum corneum</jats:italic> permeability, epidermal morphology, and expression of epidermal differentiation and host defence genes and proteins in N/TERT-HEE cultures was similar to that of primary human keratinocytes. We successfully generated N/TERT-HEEs with psoriasis or atopic dermatitis features and validated these models for drug-screening purposes. We conclude that the N/TERT keratinocyte cell lines are useful substitutes for primary human keratinocytes thereby providing a biologically relevant, unlimited cell source for <jats:italic>in vitro</jats:italic> studies on epidermal biology, inflammatory skin disease pathogenesis and therapeutics.</jats:p> Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models Scientific Reports |
| spellingShingle | Smits, Jos P. H., Niehues, Hanna, Rikken, Gijs, van Vlijmen-Willems, Ivonne M. J. J., van de Zande, Guillaume W. H. J. F., Zeeuwen, Patrick L. J. M., Schalkwijk, Joost, van den Bogaard, Ellen H., Scientific Reports, Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models, Multidisciplinary |
| title | Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_full | Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_fullStr | Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_full_unstemmed | Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_short | Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| title_sort | immortalized n/tert keratinocytes as an alternative cell source in 3d human epidermal models |
| title_unstemmed | Immortalized N/TERT keratinocytes as an alternative cell source in 3D human epidermal models |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1038/s41598-017-12041-y |