Eintrag weiter verarbeiten
Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation
Gespeichert in:
Zeitschriftentitel: | British Journal of Pharmacology |
---|---|
Personen und Körperschaften: | , , , , , , , |
In: | British Journal of Pharmacology, 154, 2008, 5, S. 1035-1046 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Wiley
|
Schlagwörter: |
author_facet |
Ryu, H Walker, J K L Kim, S Koo, N Barak, L S Noguchi, T Kang, B Y Kim, K‐M Ryu, H Walker, J K L Kim, S Koo, N Barak, L S Noguchi, T Kang, B Y Kim, K‐M |
---|---|
author |
Ryu, H Walker, J K L Kim, S Koo, N Barak, L S Noguchi, T Kang, B Y Kim, K‐M |
spellingShingle |
Ryu, H Walker, J K L Kim, S Koo, N Barak, L S Noguchi, T Kang, B Y Kim, K‐M British Journal of Pharmacology Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation Pharmacology |
author_sort |
ryu, h |
spelling |
Ryu, H Walker, J K L Kim, S Koo, N Barak, L S Noguchi, T Kang, B Y Kim, K‐M 0007-1188 1476-5381 Wiley Pharmacology http://dx.doi.org/10.1038/bjp.2008.148 <jats:sec><jats:title>Background and purpose:</jats:title><jats:p>M<jats:sub>2</jats:sub>‐type pyruvate kinase (M<jats:sub>2</jats:sub>PK) was found to interact directly with the ‘ITAM’ region of the γ chain of the high‐affinity IgE receptor (FcɛRI). Our hypothesis was that mast cell degranulation might require the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK activity.</jats:p></jats:sec><jats:sec><jats:title>Experimental approach:</jats:title><jats:p>In rat basophilic leukaemia (RBL‐2H3) cells, the effects of directly inhibiting M<jats:sub>2</jats:sub>PK or preventing the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK (disinhibition) on degranulation was measured by hexosaminidase release. Effects of blocking the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK was also assessed <jats:italic>in vivo</jats:italic> in a mouse model of allergen‐induced airway hyper‐responsiveness.</jats:p></jats:sec><jats:sec><jats:title>Key results:</jats:title><jats:p>Activation of FcɛRI in RBL‐2H3 cells caused the rapid phosphorylation of tyrosine residues in M<jats:sub>2</jats:sub>PK, associated with a decrease in M<jats:sub>2</jats:sub>PK enzymatic activity. There was an inverse correlation between M<jats:sub>2</jats:sub>PK activity and mast cell degranulation. FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK involved Src kinase, phosphatidylinositol 3‐kinase, PKC and calcium. Direct inhibition of M<jats:sub>2</jats:sub>PK potentiated FcɛRI‐mediated degranulation and prevention of the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK attenuated mast cell degranulation. Transfection of RBL‐2H3 cells with M<jats:sub>1</jats:sub>PK which prevents FcɛRI‐induced inhibition of M<jats:sub>2</jats:sub>PK, markedly reduced their degranulation and exogenous M<jats:sub>1</jats:sub>PK (i.p.) inhibited ovalbumin‐induced airway hyper‐responsiveness <jats:italic>in vivo</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions and implications:</jats:title><jats:p>We have identified a new control point and a novel biochemical pathway in the process of mast cell degranulation. Our study suggests that the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK is a crucial step in responses to allergens. Moreover, the manipulation of glycolytic processes and intermediates could provide novel strategies for the treatment of allergic diseases.</jats:p><jats:p><jats:italic>British Journal of Pharmacology</jats:italic> (2008) <jats:bold>154</jats:bold>, 1035–1046; doi:<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" xlink:href="10.1038/bjp.2008.148">10.1038/bjp.2008.148</jats:ext-link>; published online 21 April 2008</jats:p></jats:sec> Regulation of M<sub>2</sub>‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation British Journal of Pharmacology |
doi_str_mv |
10.1038/bjp.2008.148 |
facet_avail |
Online Free |
finc_class_facet |
Chemie und Pharmazie |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9ianAuMjAwOC4xNDg |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9ianAuMjAwOC4xNDg |
institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
imprint |
Wiley, 2008 |
imprint_str_mv |
Wiley, 2008 |
issn |
0007-1188 1476-5381 |
issn_str_mv |
0007-1188 1476-5381 |
language |
English |
mega_collection |
Wiley (CrossRef) |
match_str |
ryu2008regulationofm2typepyruvatekinasemediatedbythehighaffinityigereceptorsisrequiredformastcelldegranulation |
publishDateSort |
2008 |
publisher |
Wiley |
recordtype |
ai |
record_format |
ai |
series |
British Journal of Pharmacology |
source_id |
49 |
title |
Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_unstemmed |
Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_full |
Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_fullStr |
Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_full_unstemmed |
Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_short |
Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_sort |
regulation of m<sub>2</sub>‐type pyruvate kinase mediated by the high‐affinity ige receptors is required for mast cell degranulation |
topic |
Pharmacology |
url |
http://dx.doi.org/10.1038/bjp.2008.148 |
publishDate |
2008 |
physical |
1035-1046 |
description |
<jats:sec><jats:title>Background and purpose:</jats:title><jats:p>M<jats:sub>2</jats:sub>‐type pyruvate kinase (M<jats:sub>2</jats:sub>PK) was found to interact directly with the ‘ITAM’ region of the γ chain of the high‐affinity IgE receptor (FcɛRI). Our hypothesis was that mast cell degranulation might require the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK activity.</jats:p></jats:sec><jats:sec><jats:title>Experimental approach:</jats:title><jats:p>In rat basophilic leukaemia (RBL‐2H3) cells, the effects of directly inhibiting M<jats:sub>2</jats:sub>PK or preventing the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK (disinhibition) on degranulation was measured by hexosaminidase release. Effects of blocking the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK was also assessed <jats:italic>in vivo</jats:italic> in a mouse model of allergen‐induced airway hyper‐responsiveness.</jats:p></jats:sec><jats:sec><jats:title>Key results:</jats:title><jats:p>Activation of FcɛRI in RBL‐2H3 cells caused the rapid phosphorylation of tyrosine residues in M<jats:sub>2</jats:sub>PK, associated with a decrease in M<jats:sub>2</jats:sub>PK enzymatic activity. There was an inverse correlation between M<jats:sub>2</jats:sub>PK activity and mast cell degranulation. FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK involved Src kinase, phosphatidylinositol 3‐kinase, PKC and calcium. Direct inhibition of M<jats:sub>2</jats:sub>PK potentiated FcɛRI‐mediated degranulation and prevention of the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK attenuated mast cell degranulation. Transfection of RBL‐2H3 cells with M<jats:sub>1</jats:sub>PK which prevents FcɛRI‐induced inhibition of M<jats:sub>2</jats:sub>PK, markedly reduced their degranulation and exogenous M<jats:sub>1</jats:sub>PK (i.p.) inhibited ovalbumin‐induced airway hyper‐responsiveness <jats:italic>in vivo</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions and implications:</jats:title><jats:p>We have identified a new control point and a novel biochemical pathway in the process of mast cell degranulation. Our study suggests that the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK is a crucial step in responses to allergens. Moreover, the manipulation of glycolytic processes and intermediates could provide novel strategies for the treatment of allergic diseases.</jats:p><jats:p><jats:italic>British Journal of Pharmacology</jats:italic> (2008) <jats:bold>154</jats:bold>, 1035–1046; doi:<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" xlink:href="10.1038/bjp.2008.148">10.1038/bjp.2008.148</jats:ext-link>; published online 21 April 2008</jats:p></jats:sec> |
container_issue |
5 |
container_start_page |
1035 |
container_title |
British Journal of Pharmacology |
container_volume |
154 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792338927860842497 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T15:39:59.525Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Regulation+of+M2%E2%80%90type+pyruvate+kinase+mediated+by+the+high%E2%80%90affinity+IgE+receptors+is+required+for+mast+cell+degranulation&rft.date=2008-07-01&genre=article&issn=1476-5381&volume=154&issue=5&spage=1035&epage=1046&pages=1035-1046&jtitle=British+Journal+of+Pharmacology&atitle=Regulation+of+M%3Csub%3E2%3C%2Fsub%3E%E2%80%90type+pyruvate+kinase+mediated+by+the+high%E2%80%90affinity+IgE+receptors+is+required+for+mast+cell+degranulation&aulast=Kim&aufirst=K%E2%80%90M&rft_id=info%3Adoi%2F10.1038%2Fbjp.2008.148&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792338927860842497 |
author | Ryu, H, Walker, J K L, Kim, S, Koo, N, Barak, L S, Noguchi, T, Kang, B Y, Kim, K‐M |
author_facet | Ryu, H, Walker, J K L, Kim, S, Koo, N, Barak, L S, Noguchi, T, Kang, B Y, Kim, K‐M, Ryu, H, Walker, J K L, Kim, S, Koo, N, Barak, L S, Noguchi, T, Kang, B Y, Kim, K‐M |
author_sort | ryu, h |
container_issue | 5 |
container_start_page | 1035 |
container_title | British Journal of Pharmacology |
container_volume | 154 |
description | <jats:sec><jats:title>Background and purpose:</jats:title><jats:p>M<jats:sub>2</jats:sub>‐type pyruvate kinase (M<jats:sub>2</jats:sub>PK) was found to interact directly with the ‘ITAM’ region of the γ chain of the high‐affinity IgE receptor (FcɛRI). Our hypothesis was that mast cell degranulation might require the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK activity.</jats:p></jats:sec><jats:sec><jats:title>Experimental approach:</jats:title><jats:p>In rat basophilic leukaemia (RBL‐2H3) cells, the effects of directly inhibiting M<jats:sub>2</jats:sub>PK or preventing the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK (disinhibition) on degranulation was measured by hexosaminidase release. Effects of blocking the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK was also assessed <jats:italic>in vivo</jats:italic> in a mouse model of allergen‐induced airway hyper‐responsiveness.</jats:p></jats:sec><jats:sec><jats:title>Key results:</jats:title><jats:p>Activation of FcɛRI in RBL‐2H3 cells caused the rapid phosphorylation of tyrosine residues in M<jats:sub>2</jats:sub>PK, associated with a decrease in M<jats:sub>2</jats:sub>PK enzymatic activity. There was an inverse correlation between M<jats:sub>2</jats:sub>PK activity and mast cell degranulation. FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK involved Src kinase, phosphatidylinositol 3‐kinase, PKC and calcium. Direct inhibition of M<jats:sub>2</jats:sub>PK potentiated FcɛRI‐mediated degranulation and prevention of the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK attenuated mast cell degranulation. Transfection of RBL‐2H3 cells with M<jats:sub>1</jats:sub>PK which prevents FcɛRI‐induced inhibition of M<jats:sub>2</jats:sub>PK, markedly reduced their degranulation and exogenous M<jats:sub>1</jats:sub>PK (i.p.) inhibited ovalbumin‐induced airway hyper‐responsiveness <jats:italic>in vivo</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions and implications:</jats:title><jats:p>We have identified a new control point and a novel biochemical pathway in the process of mast cell degranulation. Our study suggests that the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK is a crucial step in responses to allergens. Moreover, the manipulation of glycolytic processes and intermediates could provide novel strategies for the treatment of allergic diseases.</jats:p><jats:p><jats:italic>British Journal of Pharmacology</jats:italic> (2008) <jats:bold>154</jats:bold>, 1035–1046; doi:<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" xlink:href="10.1038/bjp.2008.148">10.1038/bjp.2008.148</jats:ext-link>; published online 21 April 2008</jats:p></jats:sec> |
doi_str_mv | 10.1038/bjp.2008.148 |
facet_avail | Online, Free |
finc_class_facet | Chemie und Pharmazie |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAzOC9ianAuMjAwOC4xNDg |
imprint | Wiley, 2008 |
imprint_str_mv | Wiley, 2008 |
institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
issn | 0007-1188, 1476-5381 |
issn_str_mv | 0007-1188, 1476-5381 |
language | English |
last_indexed | 2024-03-01T15:39:59.525Z |
match_str | ryu2008regulationofm2typepyruvatekinasemediatedbythehighaffinityigereceptorsisrequiredformastcelldegranulation |
mega_collection | Wiley (CrossRef) |
physical | 1035-1046 |
publishDate | 2008 |
publishDateSort | 2008 |
publisher | Wiley |
record_format | ai |
recordtype | ai |
series | British Journal of Pharmacology |
source_id | 49 |
spelling | Ryu, H Walker, J K L Kim, S Koo, N Barak, L S Noguchi, T Kang, B Y Kim, K‐M 0007-1188 1476-5381 Wiley Pharmacology http://dx.doi.org/10.1038/bjp.2008.148 <jats:sec><jats:title>Background and purpose:</jats:title><jats:p>M<jats:sub>2</jats:sub>‐type pyruvate kinase (M<jats:sub>2</jats:sub>PK) was found to interact directly with the ‘ITAM’ region of the γ chain of the high‐affinity IgE receptor (FcɛRI). Our hypothesis was that mast cell degranulation might require the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK activity.</jats:p></jats:sec><jats:sec><jats:title>Experimental approach:</jats:title><jats:p>In rat basophilic leukaemia (RBL‐2H3) cells, the effects of directly inhibiting M<jats:sub>2</jats:sub>PK or preventing the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK (disinhibition) on degranulation was measured by hexosaminidase release. Effects of blocking the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK was also assessed <jats:italic>in vivo</jats:italic> in a mouse model of allergen‐induced airway hyper‐responsiveness.</jats:p></jats:sec><jats:sec><jats:title>Key results:</jats:title><jats:p>Activation of FcɛRI in RBL‐2H3 cells caused the rapid phosphorylation of tyrosine residues in M<jats:sub>2</jats:sub>PK, associated with a decrease in M<jats:sub>2</jats:sub>PK enzymatic activity. There was an inverse correlation between M<jats:sub>2</jats:sub>PK activity and mast cell degranulation. FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK involved Src kinase, phosphatidylinositol 3‐kinase, PKC and calcium. Direct inhibition of M<jats:sub>2</jats:sub>PK potentiated FcɛRI‐mediated degranulation and prevention of the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK attenuated mast cell degranulation. Transfection of RBL‐2H3 cells with M<jats:sub>1</jats:sub>PK which prevents FcɛRI‐induced inhibition of M<jats:sub>2</jats:sub>PK, markedly reduced their degranulation and exogenous M<jats:sub>1</jats:sub>PK (i.p.) inhibited ovalbumin‐induced airway hyper‐responsiveness <jats:italic>in vivo</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions and implications:</jats:title><jats:p>We have identified a new control point and a novel biochemical pathway in the process of mast cell degranulation. Our study suggests that the FcɛRI‐mediated inhibition of M<jats:sub>2</jats:sub>PK is a crucial step in responses to allergens. Moreover, the manipulation of glycolytic processes and intermediates could provide novel strategies for the treatment of allergic diseases.</jats:p><jats:p><jats:italic>British Journal of Pharmacology</jats:italic> (2008) <jats:bold>154</jats:bold>, 1035–1046; doi:<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" xlink:href="10.1038/bjp.2008.148">10.1038/bjp.2008.148</jats:ext-link>; published online 21 April 2008</jats:p></jats:sec> Regulation of M<sub>2</sub>‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation British Journal of Pharmacology |
spellingShingle | Ryu, H, Walker, J K L, Kim, S, Koo, N, Barak, L S, Noguchi, T, Kang, B Y, Kim, K‐M, British Journal of Pharmacology, Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation, Pharmacology |
title | Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_full | Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_fullStr | Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_full_unstemmed | Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_short | Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
title_sort | regulation of m<sub>2</sub>‐type pyruvate kinase mediated by the high‐affinity ige receptors is required for mast cell degranulation |
title_unstemmed | Regulation of M2‐type pyruvate kinase mediated by the high‐affinity IgE receptors is required for mast cell degranulation |
topic | Pharmacology |
url | http://dx.doi.org/10.1038/bjp.2008.148 |