Eintrag weiter verarbeiten
Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells
Gespeichert in:
Zeitschriftentitel: | Stem Cells |
---|---|
Personen und Körperschaften: | , , , , , , , |
In: | Stem Cells, 28, 2010, 10, S. 1848-1855 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Oxford University Press (OUP)
|
Schlagwörter: |
author_facet |
Chung, Tung-Liang Brena, Romulo M. Kolle, Gabriel Grimmond, Sean M. Berman, Benjamin P. Laird, Peter W. Pera, Martin F. Wolvetang, Ernst Jurgen Chung, Tung-Liang Brena, Romulo M. Kolle, Gabriel Grimmond, Sean M. Berman, Benjamin P. Laird, Peter W. Pera, Martin F. Wolvetang, Ernst Jurgen |
---|---|
author |
Chung, Tung-Liang Brena, Romulo M. Kolle, Gabriel Grimmond, Sean M. Berman, Benjamin P. Laird, Peter W. Pera, Martin F. Wolvetang, Ernst Jurgen |
spellingShingle |
Chung, Tung-Liang Brena, Romulo M. Kolle, Gabriel Grimmond, Sean M. Berman, Benjamin P. Laird, Peter W. Pera, Martin F. Wolvetang, Ernst Jurgen Stem Cells Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells Cell Biology Developmental Biology Molecular Medicine |
author_sort |
chung, tung-liang |
spelling |
Chung, Tung-Liang Brena, Romulo M. Kolle, Gabriel Grimmond, Sean M. Berman, Benjamin P. Laird, Peter W. Pera, Martin F. Wolvetang, Ernst Jurgen 1066-5099 1549-4918 Oxford University Press (OUP) Cell Biology Developmental Biology Molecular Medicine http://dx.doi.org/10.1002/stem.493 <jats:title>Abstract</jats:title> <jats:p>Vitamin C (ascorbate) is a widely used medium supplement in embryonic stem cell culture. Here, we show that ascorbate causes widespread, consistent, and remarkably specific DNA demethylation of 1,847 genes in human embryonic stem cells (hESCs), including important stem cell genes, with a clear bias toward demethylation at CpG island boundaries. We show that a subset of these DNA demethylated genes displays concomitant gene expression changes and that the position of the demethylated CpGs relative to the transcription start site is correlated to such changes. We further show that the ascorbate-demethylated gene set not only overlaps with gene sets that have bivalent marks, but also with the gene sets that are demethylated during differentiation of hESCs and during reprogramming of fibroblasts to induced pluritotent stem cells (iPSCs). Our data thus identify a novel link between ascorbate-mediated signaling and specific epigenetic changes in hESCs that might impact on pluripotency and reprogramming pathways.</jats:p> Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells Stem Cells |
doi_str_mv |
10.1002/stem.493 |
facet_avail |
Online Free |
finc_class_facet |
Biologie Medizin |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9zdGVtLjQ5Mw |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9zdGVtLjQ5Mw |
institution |
DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 |
imprint |
Oxford University Press (OUP), 2010 |
imprint_str_mv |
Oxford University Press (OUP), 2010 |
issn |
1066-5099 1549-4918 |
issn_str_mv |
1066-5099 1549-4918 |
language |
English |
mega_collection |
Oxford University Press (OUP) (CrossRef) |
match_str |
chung2010vitamincpromoteswidespreadyetspecificdnademethylationoftheepigenomeinhumanembryonicstemcells |
publishDateSort |
2010 |
publisher |
Oxford University Press (OUP) |
recordtype |
ai |
record_format |
ai |
series |
Stem Cells |
source_id |
49 |
title |
Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_unstemmed |
Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_full |
Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_fullStr |
Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_full_unstemmed |
Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_short |
Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_sort |
vitamin c promotes widespread yet specific dna demethylation of the epigenome in human embryonic stem cells |
topic |
Cell Biology Developmental Biology Molecular Medicine |
url |
http://dx.doi.org/10.1002/stem.493 |
publishDate |
2010 |
physical |
1848-1855 |
description |
<jats:title>Abstract</jats:title>
<jats:p>Vitamin C (ascorbate) is a widely used medium supplement in embryonic stem cell culture. Here, we show that ascorbate causes widespread, consistent, and remarkably specific DNA demethylation of 1,847 genes in human embryonic stem cells (hESCs), including important stem cell genes, with a clear bias toward demethylation at CpG island boundaries. We show that a subset of these DNA demethylated genes displays concomitant gene expression changes and that the position of the demethylated CpGs relative to the transcription start site is correlated to such changes. We further show that the ascorbate-demethylated gene set not only overlaps with gene sets that have bivalent marks, but also with the gene sets that are demethylated during differentiation of hESCs and during reprogramming of fibroblasts to induced pluritotent stem cells (iPSCs). Our data thus identify a novel link between ascorbate-mediated signaling and specific epigenetic changes in hESCs that might impact on pluripotency and reprogramming pathways.</jats:p> |
container_issue |
10 |
container_start_page |
1848 |
container_title |
Stem Cells |
container_volume |
28 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792342657233584131 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T16:39:13.815Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Vitamin+C+Promotes+Widespread+Yet+Specific+DNA+Demethylation+of+the+Epigenome+in+Human+Embryonic+Stem+Cells&rft.date=2010-10-01&genre=article&issn=1549-4918&volume=28&issue=10&spage=1848&epage=1855&pages=1848-1855&jtitle=Stem+Cells&atitle=Vitamin+C+Promotes+Widespread+Yet+Specific+DNA+Demethylation+of+the+Epigenome+in+Human+Embryonic+Stem+Cells&aulast=Wolvetang&aufirst=Ernst+Jurgen&rft_id=info%3Adoi%2F10.1002%2Fstem.493&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792342657233584131 |
author | Chung, Tung-Liang, Brena, Romulo M., Kolle, Gabriel, Grimmond, Sean M., Berman, Benjamin P., Laird, Peter W., Pera, Martin F., Wolvetang, Ernst Jurgen |
author_facet | Chung, Tung-Liang, Brena, Romulo M., Kolle, Gabriel, Grimmond, Sean M., Berman, Benjamin P., Laird, Peter W., Pera, Martin F., Wolvetang, Ernst Jurgen, Chung, Tung-Liang, Brena, Romulo M., Kolle, Gabriel, Grimmond, Sean M., Berman, Benjamin P., Laird, Peter W., Pera, Martin F., Wolvetang, Ernst Jurgen |
author_sort | chung, tung-liang |
container_issue | 10 |
container_start_page | 1848 |
container_title | Stem Cells |
container_volume | 28 |
description | <jats:title>Abstract</jats:title> <jats:p>Vitamin C (ascorbate) is a widely used medium supplement in embryonic stem cell culture. Here, we show that ascorbate causes widespread, consistent, and remarkably specific DNA demethylation of 1,847 genes in human embryonic stem cells (hESCs), including important stem cell genes, with a clear bias toward demethylation at CpG island boundaries. We show that a subset of these DNA demethylated genes displays concomitant gene expression changes and that the position of the demethylated CpGs relative to the transcription start site is correlated to such changes. We further show that the ascorbate-demethylated gene set not only overlaps with gene sets that have bivalent marks, but also with the gene sets that are demethylated during differentiation of hESCs and during reprogramming of fibroblasts to induced pluritotent stem cells (iPSCs). Our data thus identify a novel link between ascorbate-mediated signaling and specific epigenetic changes in hESCs that might impact on pluripotency and reprogramming pathways.</jats:p> |
doi_str_mv | 10.1002/stem.493 |
facet_avail | Online, Free |
finc_class_facet | Biologie, Medizin |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9zdGVtLjQ5Mw |
imprint | Oxford University Press (OUP), 2010 |
imprint_str_mv | Oxford University Press (OUP), 2010 |
institution | DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15 |
issn | 1066-5099, 1549-4918 |
issn_str_mv | 1066-5099, 1549-4918 |
language | English |
last_indexed | 2024-03-01T16:39:13.815Z |
match_str | chung2010vitamincpromoteswidespreadyetspecificdnademethylationoftheepigenomeinhumanembryonicstemcells |
mega_collection | Oxford University Press (OUP) (CrossRef) |
physical | 1848-1855 |
publishDate | 2010 |
publishDateSort | 2010 |
publisher | Oxford University Press (OUP) |
record_format | ai |
recordtype | ai |
series | Stem Cells |
source_id | 49 |
spelling | Chung, Tung-Liang Brena, Romulo M. Kolle, Gabriel Grimmond, Sean M. Berman, Benjamin P. Laird, Peter W. Pera, Martin F. Wolvetang, Ernst Jurgen 1066-5099 1549-4918 Oxford University Press (OUP) Cell Biology Developmental Biology Molecular Medicine http://dx.doi.org/10.1002/stem.493 <jats:title>Abstract</jats:title> <jats:p>Vitamin C (ascorbate) is a widely used medium supplement in embryonic stem cell culture. Here, we show that ascorbate causes widespread, consistent, and remarkably specific DNA demethylation of 1,847 genes in human embryonic stem cells (hESCs), including important stem cell genes, with a clear bias toward demethylation at CpG island boundaries. We show that a subset of these DNA demethylated genes displays concomitant gene expression changes and that the position of the demethylated CpGs relative to the transcription start site is correlated to such changes. We further show that the ascorbate-demethylated gene set not only overlaps with gene sets that have bivalent marks, but also with the gene sets that are demethylated during differentiation of hESCs and during reprogramming of fibroblasts to induced pluritotent stem cells (iPSCs). Our data thus identify a novel link between ascorbate-mediated signaling and specific epigenetic changes in hESCs that might impact on pluripotency and reprogramming pathways.</jats:p> Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells Stem Cells |
spellingShingle | Chung, Tung-Liang, Brena, Romulo M., Kolle, Gabriel, Grimmond, Sean M., Berman, Benjamin P., Laird, Peter W., Pera, Martin F., Wolvetang, Ernst Jurgen, Stem Cells, Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells, Cell Biology, Developmental Biology, Molecular Medicine |
title | Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_full | Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_fullStr | Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_full_unstemmed | Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_short | Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
title_sort | vitamin c promotes widespread yet specific dna demethylation of the epigenome in human embryonic stem cells |
title_unstemmed | Vitamin C Promotes Widespread Yet Specific DNA Demethylation of the Epigenome in Human Embryonic Stem Cells |
topic | Cell Biology, Developmental Biology, Molecular Medicine |
url | http://dx.doi.org/10.1002/stem.493 |