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Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases
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| Zeitschriftentitel: | International Journal of Cancer |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , |
| In: | International Journal of Cancer, 118, 2006, 1, S. 74-89 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Bandapalli, Obul Reddy Geheeb, Martina Kobelt, Dennis Kuehnle, Katrin Elezkurtaj, Sefer Herrmann, Jens Gressner, Axel M. Weiskirchen, Ralf Beule, Dieter Blüthgen, Nils Herzel, Hanspeter Franke, Claudia Brand, Karsten Bandapalli, Obul Reddy Geheeb, Martina Kobelt, Dennis Kuehnle, Katrin Elezkurtaj, Sefer Herrmann, Jens Gressner, Axel M. Weiskirchen, Ralf Beule, Dieter Blüthgen, Nils Herzel, Hanspeter Franke, Claudia Brand, Karsten |
|---|---|
| author |
Bandapalli, Obul Reddy Geheeb, Martina Kobelt, Dennis Kuehnle, Katrin Elezkurtaj, Sefer Herrmann, Jens Gressner, Axel M. Weiskirchen, Ralf Beule, Dieter Blüthgen, Nils Herzel, Hanspeter Franke, Claudia Brand, Karsten |
| spellingShingle |
Bandapalli, Obul Reddy Geheeb, Martina Kobelt, Dennis Kuehnle, Katrin Elezkurtaj, Sefer Herrmann, Jens Gressner, Axel M. Weiskirchen, Ralf Beule, Dieter Blüthgen, Nils Herzel, Hanspeter Franke, Claudia Brand, Karsten International Journal of Cancer Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases Cancer Research Oncology |
| author_sort |
bandapalli, obul reddy |
| spelling |
Bandapalli, Obul Reddy Geheeb, Martina Kobelt, Dennis Kuehnle, Katrin Elezkurtaj, Sefer Herrmann, Jens Gressner, Axel M. Weiskirchen, Ralf Beule, Dieter Blüthgen, Nils Herzel, Hanspeter Franke, Claudia Brand, Karsten 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.21307 <jats:title>Abstract</jats:title><jats:p>Host cell reactions are a crucial determinant for tumor invasion. We analyzed on a genomewide scale gene expression differences between microdissected tissues taken from unaffected liver tissue of a human colorectal tumor (LS174) growing in the livers of nude mice and tissue from the host part of the invasive front. Due to the low degree of interspecies cross‐hybridization of 15% as determined on Affymetrix microarrays, our xenograft model allowed for the distinction of genes of murine <jats:italic>versus</jats:italic> human origin even if the respective tissues could not be isolated separately. Using the gene ontology (GO) classification, we were able to determine patterns of up‐ and downregulated genes in the liver part of the invasive front. We observed a pronounced overrepresentation, <jats:italic>e.g.</jats:italic>, of the GO terms “extracellular matrix,” “cell communication,” “response to biotic stimulus,” “structural molecule activity” and “cell growth,” indicating a very pronounced host cell response to tumor invasion. On the single gene level, hepatic stellate cell (HSC) activation markers were overrepresented in the liver part of the invasion front. Immunohistochemistry and qPCR confirmed an activation of HSC as well as an increased number of HSC in the invasive front as compared to the noninvaded liver tissue. In summary, our data demonstrate the feasibility of an interspecies differential gene expression approach on a genomewide scale. © 2005 Wiley‐Liss, Inc.</jats:p> Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases International Journal of Cancer |
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| title |
Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_unstemmed |
Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_full |
Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_fullStr |
Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_full_unstemmed |
Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_short |
Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_sort |
global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| topic |
Cancer Research Oncology |
| url |
http://dx.doi.org/10.1002/ijc.21307 |
| publishDate |
2006 |
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74-89 |
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<jats:title>Abstract</jats:title><jats:p>Host cell reactions are a crucial determinant for tumor invasion. We analyzed on a genomewide scale gene expression differences between microdissected tissues taken from unaffected liver tissue of a human colorectal tumor (LS174) growing in the livers of nude mice and tissue from the host part of the invasive front. Due to the low degree of interspecies cross‐hybridization of 15% as determined on Affymetrix microarrays, our xenograft model allowed for the distinction of genes of murine <jats:italic>versus</jats:italic> human origin even if the respective tissues could not be isolated separately. Using the gene ontology (GO) classification, we were able to determine patterns of up‐ and downregulated genes in the liver part of the invasive front. We observed a pronounced overrepresentation, <jats:italic>e.g.</jats:italic>, of the GO terms “extracellular matrix,” “cell communication,” “response to biotic stimulus,” “structural molecule activity” and “cell growth,” indicating a very pronounced host cell response to tumor invasion. On the single gene level, hepatic stellate cell (HSC) activation markers were overrepresented in the liver part of the invasion front. Immunohistochemistry and qPCR confirmed an activation of HSC as well as an increased number of HSC in the invasive front as compared to the noninvaded liver tissue. In summary, our data demonstrate the feasibility of an interspecies differential gene expression approach on a genomewide scale. © 2005 Wiley‐Liss, Inc.</jats:p> |
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| author | Bandapalli, Obul Reddy, Geheeb, Martina, Kobelt, Dennis, Kuehnle, Katrin, Elezkurtaj, Sefer, Herrmann, Jens, Gressner, Axel M., Weiskirchen, Ralf, Beule, Dieter, Blüthgen, Nils, Herzel, Hanspeter, Franke, Claudia, Brand, Karsten |
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| description | <jats:title>Abstract</jats:title><jats:p>Host cell reactions are a crucial determinant for tumor invasion. We analyzed on a genomewide scale gene expression differences between microdissected tissues taken from unaffected liver tissue of a human colorectal tumor (LS174) growing in the livers of nude mice and tissue from the host part of the invasive front. Due to the low degree of interspecies cross‐hybridization of 15% as determined on Affymetrix microarrays, our xenograft model allowed for the distinction of genes of murine <jats:italic>versus</jats:italic> human origin even if the respective tissues could not be isolated separately. Using the gene ontology (GO) classification, we were able to determine patterns of up‐ and downregulated genes in the liver part of the invasive front. We observed a pronounced overrepresentation, <jats:italic>e.g.</jats:italic>, of the GO terms “extracellular matrix,” “cell communication,” “response to biotic stimulus,” “structural molecule activity” and “cell growth,” indicating a very pronounced host cell response to tumor invasion. On the single gene level, hepatic stellate cell (HSC) activation markers were overrepresented in the liver part of the invasion front. Immunohistochemistry and qPCR confirmed an activation of HSC as well as an increased number of HSC in the invasive front as compared to the noninvaded liver tissue. In summary, our data demonstrate the feasibility of an interspecies differential gene expression approach on a genomewide scale. © 2005 Wiley‐Liss, Inc.</jats:p> |
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| spelling | Bandapalli, Obul Reddy Geheeb, Martina Kobelt, Dennis Kuehnle, Katrin Elezkurtaj, Sefer Herrmann, Jens Gressner, Axel M. Weiskirchen, Ralf Beule, Dieter Blüthgen, Nils Herzel, Hanspeter Franke, Claudia Brand, Karsten 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.21307 <jats:title>Abstract</jats:title><jats:p>Host cell reactions are a crucial determinant for tumor invasion. We analyzed on a genomewide scale gene expression differences between microdissected tissues taken from unaffected liver tissue of a human colorectal tumor (LS174) growing in the livers of nude mice and tissue from the host part of the invasive front. Due to the low degree of interspecies cross‐hybridization of 15% as determined on Affymetrix microarrays, our xenograft model allowed for the distinction of genes of murine <jats:italic>versus</jats:italic> human origin even if the respective tissues could not be isolated separately. Using the gene ontology (GO) classification, we were able to determine patterns of up‐ and downregulated genes in the liver part of the invasive front. We observed a pronounced overrepresentation, <jats:italic>e.g.</jats:italic>, of the GO terms “extracellular matrix,” “cell communication,” “response to biotic stimulus,” “structural molecule activity” and “cell growth,” indicating a very pronounced host cell response to tumor invasion. On the single gene level, hepatic stellate cell (HSC) activation markers were overrepresented in the liver part of the invasion front. Immunohistochemistry and qPCR confirmed an activation of HSC as well as an increased number of HSC in the invasive front as compared to the noninvaded liver tissue. In summary, our data demonstrate the feasibility of an interspecies differential gene expression approach on a genomewide scale. © 2005 Wiley‐Liss, Inc.</jats:p> Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases International Journal of Cancer |
| spellingShingle | Bandapalli, Obul Reddy, Geheeb, Martina, Kobelt, Dennis, Kuehnle, Katrin, Elezkurtaj, Sefer, Herrmann, Jens, Gressner, Axel M., Weiskirchen, Ralf, Beule, Dieter, Blüthgen, Nils, Herzel, Hanspeter, Franke, Claudia, Brand, Karsten, International Journal of Cancer, Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases, Cancer Research, Oncology |
| title | Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_full | Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_fullStr | Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_full_unstemmed | Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_short | Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_sort | global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| title_unstemmed | Global analysis of host tissue gene expression in the invasive front of colorectal liver metastases |
| topic | Cancer Research, Oncology |
| url | http://dx.doi.org/10.1002/ijc.21307 |