Eintrag weiter verarbeiten
Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma
Gespeichert in:
| Zeitschriftentitel: | International Journal of Cancer |
|---|---|
| Personen und Körperschaften: | , , |
| In: | International Journal of Cancer, 104, 2003, 6, S. 735-744 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Huang, Ying de la Chapelle, Albert Pellegata, Natalia S. Huang, Ying de la Chapelle, Albert Pellegata, Natalia S. |
|---|---|
| author |
Huang, Ying de la Chapelle, Albert Pellegata, Natalia S. |
| spellingShingle |
Huang, Ying de la Chapelle, Albert Pellegata, Natalia S. International Journal of Cancer Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma Cancer Research Oncology |
| author_sort |
huang, ying |
| spelling |
Huang, Ying de la Chapelle, Albert Pellegata, Natalia S. 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.11006 <jats:title>Abstract</jats:title><jats:p>We previously obtained gene expression profiles of 8 matched papillary thyroid carcinoma (PTC) and normal tissues using DNA microarrays. To identify novel tumor suppressor genes involved in thyroid carcinogenesis, we here analyze genes showing lower expression in PTC tumors than in normal thyroid tissues. A search for loss of heterozygosity (LOH) in 49 regions that harbor consistently down‐regulated genes revealed LOH in only 4 regions and in just a very small number of tumors. To determine whether the underexpression might be due to promoter methylation, we used combined bisulfite restriction analysis and bisulfite sequencing to study 7 underexpressed genes. Loss of expression of <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> is accompanied by hypermethylation in the 5′ regions of these genes, but methylation was not seen in other genes tested. Combined treatment with the DNA methyltransferase inhibitor 5‐aza‐2′‐deoxycytidine (5‐Aza‐dC) and the histone deacetylase inhibitor trichostatin A (TSA) resulted in demethylation and re‐expression of the <jats:italic>MT1G</jats:italic> gene in the cell line K2. Treatment with 5‐Aza‐dC alone restored <jats:italic>CRABP1</jats:italic> expression in a colorectal cancer cell line, SW48. In conclusion, LOH is a remarkably rare mechanism of loss of gene function in PTC. In contrast, hypermethylation of promoter CpG islands seems to occur at higher frequency. <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> are novel genes that are likely involved in the pathogenesis of sporadic PTC. © 2003 Wiley‐Liss, Inc.</jats:p> Hypermethylation, but not LOH, is associated with the low expression of <i>MT1G</i> and <i>CRABP1</i> in papillary thyroid carcinoma International Journal of Cancer |
| doi_str_mv |
10.1002/ijc.11006 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9pamMuMTEwMDY |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9pamMuMTEwMDY |
| institution |
DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 |
| imprint |
Wiley, 2003 |
| imprint_str_mv |
Wiley, 2003 |
| issn |
1097-0215 0020-7136 |
| issn_str_mv |
1097-0215 0020-7136 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
huang2003hypermethylationbutnotlohisassociatedwiththelowexpressionofmt1gandcrabp1inpapillarythyroidcarcinoma |
| publishDateSort |
2003 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
International Journal of Cancer |
| source_id |
49 |
| title |
Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_unstemmed |
Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_full |
Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_fullStr |
Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_full_unstemmed |
Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_short |
Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_sort |
hypermethylation, but not loh, is associated with the low expression of <i>mt1g</i> and <i>crabp1</i> in papillary thyroid carcinoma |
| topic |
Cancer Research Oncology |
| url |
http://dx.doi.org/10.1002/ijc.11006 |
| publishDate |
2003 |
| physical |
735-744 |
| description |
<jats:title>Abstract</jats:title><jats:p>We previously obtained gene expression profiles of 8 matched papillary thyroid carcinoma (PTC) and normal tissues using DNA microarrays. To identify novel tumor suppressor genes involved in thyroid carcinogenesis, we here analyze genes showing lower expression in PTC tumors than in normal thyroid tissues. A search for loss of heterozygosity (LOH) in 49 regions that harbor consistently down‐regulated genes revealed LOH in only 4 regions and in just a very small number of tumors. To determine whether the underexpression might be due to promoter methylation, we used combined bisulfite restriction analysis and bisulfite sequencing to study 7 underexpressed genes. Loss of expression of <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> is accompanied by hypermethylation in the 5′ regions of these genes, but methylation was not seen in other genes tested. Combined treatment with the DNA methyltransferase inhibitor 5‐aza‐2′‐deoxycytidine (5‐Aza‐dC) and the histone deacetylase inhibitor trichostatin A (TSA) resulted in demethylation and re‐expression of the <jats:italic>MT1G</jats:italic> gene in the cell line K2. Treatment with 5‐Aza‐dC alone restored <jats:italic>CRABP1</jats:italic> expression in a colorectal cancer cell line, SW48. In conclusion, LOH is a remarkably rare mechanism of loss of gene function in PTC. In contrast, hypermethylation of promoter CpG islands seems to occur at higher frequency. <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> are novel genes that are likely involved in the pathogenesis of sporadic PTC. © 2003 Wiley‐Liss, Inc.</jats:p> |
| container_issue |
6 |
| container_start_page |
735 |
| container_title |
International Journal of Cancer |
| container_volume |
104 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792347961861079052 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T18:03:35.97Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Hypermethylation%2C+but+not+LOH%2C+is+associated+with+the+low+expression+of+MT1G+and+CRABP1+in+papillary+thyroid+carcinoma&rft.date=2003-05-10&genre=article&issn=1097-0215&volume=104&issue=6&spage=735&epage=744&pages=735-744&jtitle=International+Journal+of+Cancer&atitle=Hypermethylation%2C+but+not+LOH%2C+is+associated+with+the+low+expression+of+%3Ci%3EMT1G%3C%2Fi%3E+and+%3Ci%3ECRABP1%3C%2Fi%3E+in+papillary+thyroid+carcinoma&aulast=Pellegata&aufirst=Natalia+S.&rft_id=info%3Adoi%2F10.1002%2Fijc.11006&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792347961861079052 |
| author | Huang, Ying, de la Chapelle, Albert, Pellegata, Natalia S. |
| author_facet | Huang, Ying, de la Chapelle, Albert, Pellegata, Natalia S., Huang, Ying, de la Chapelle, Albert, Pellegata, Natalia S. |
| author_sort | huang, ying |
| container_issue | 6 |
| container_start_page | 735 |
| container_title | International Journal of Cancer |
| container_volume | 104 |
| description | <jats:title>Abstract</jats:title><jats:p>We previously obtained gene expression profiles of 8 matched papillary thyroid carcinoma (PTC) and normal tissues using DNA microarrays. To identify novel tumor suppressor genes involved in thyroid carcinogenesis, we here analyze genes showing lower expression in PTC tumors than in normal thyroid tissues. A search for loss of heterozygosity (LOH) in 49 regions that harbor consistently down‐regulated genes revealed LOH in only 4 regions and in just a very small number of tumors. To determine whether the underexpression might be due to promoter methylation, we used combined bisulfite restriction analysis and bisulfite sequencing to study 7 underexpressed genes. Loss of expression of <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> is accompanied by hypermethylation in the 5′ regions of these genes, but methylation was not seen in other genes tested. Combined treatment with the DNA methyltransferase inhibitor 5‐aza‐2′‐deoxycytidine (5‐Aza‐dC) and the histone deacetylase inhibitor trichostatin A (TSA) resulted in demethylation and re‐expression of the <jats:italic>MT1G</jats:italic> gene in the cell line K2. Treatment with 5‐Aza‐dC alone restored <jats:italic>CRABP1</jats:italic> expression in a colorectal cancer cell line, SW48. In conclusion, LOH is a remarkably rare mechanism of loss of gene function in PTC. In contrast, hypermethylation of promoter CpG islands seems to occur at higher frequency. <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> are novel genes that are likely involved in the pathogenesis of sporadic PTC. © 2003 Wiley‐Liss, Inc.</jats:p> |
| doi_str_mv | 10.1002/ijc.11006 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9pamMuMTEwMDY |
| imprint | Wiley, 2003 |
| imprint_str_mv | Wiley, 2003 |
| institution | DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1 |
| issn | 1097-0215, 0020-7136 |
| issn_str_mv | 1097-0215, 0020-7136 |
| language | English |
| last_indexed | 2024-03-01T18:03:35.97Z |
| match_str | huang2003hypermethylationbutnotlohisassociatedwiththelowexpressionofmt1gandcrabp1inpapillarythyroidcarcinoma |
| mega_collection | Wiley (CrossRef) |
| physical | 735-744 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | International Journal of Cancer |
| source_id | 49 |
| spelling | Huang, Ying de la Chapelle, Albert Pellegata, Natalia S. 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.11006 <jats:title>Abstract</jats:title><jats:p>We previously obtained gene expression profiles of 8 matched papillary thyroid carcinoma (PTC) and normal tissues using DNA microarrays. To identify novel tumor suppressor genes involved in thyroid carcinogenesis, we here analyze genes showing lower expression in PTC tumors than in normal thyroid tissues. A search for loss of heterozygosity (LOH) in 49 regions that harbor consistently down‐regulated genes revealed LOH in only 4 regions and in just a very small number of tumors. To determine whether the underexpression might be due to promoter methylation, we used combined bisulfite restriction analysis and bisulfite sequencing to study 7 underexpressed genes. Loss of expression of <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> is accompanied by hypermethylation in the 5′ regions of these genes, but methylation was not seen in other genes tested. Combined treatment with the DNA methyltransferase inhibitor 5‐aza‐2′‐deoxycytidine (5‐Aza‐dC) and the histone deacetylase inhibitor trichostatin A (TSA) resulted in demethylation and re‐expression of the <jats:italic>MT1G</jats:italic> gene in the cell line K2. Treatment with 5‐Aza‐dC alone restored <jats:italic>CRABP1</jats:italic> expression in a colorectal cancer cell line, SW48. In conclusion, LOH is a remarkably rare mechanism of loss of gene function in PTC. In contrast, hypermethylation of promoter CpG islands seems to occur at higher frequency. <jats:italic>MT1G</jats:italic> and <jats:italic>CRABP1</jats:italic> are novel genes that are likely involved in the pathogenesis of sporadic PTC. © 2003 Wiley‐Liss, Inc.</jats:p> Hypermethylation, but not LOH, is associated with the low expression of <i>MT1G</i> and <i>CRABP1</i> in papillary thyroid carcinoma International Journal of Cancer |
| spellingShingle | Huang, Ying, de la Chapelle, Albert, Pellegata, Natalia S., International Journal of Cancer, Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma, Cancer Research, Oncology |
| title | Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_full | Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_fullStr | Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_full_unstemmed | Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_short | Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| title_sort | hypermethylation, but not loh, is associated with the low expression of <i>mt1g</i> and <i>crabp1</i> in papillary thyroid carcinoma |
| title_unstemmed | Hypermethylation, but not LOH, is associated with the low expression of MT1G and CRABP1 in papillary thyroid carcinoma |
| topic | Cancer Research, Oncology |
| url | http://dx.doi.org/10.1002/ijc.11006 |