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Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells
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| Zeitschriftentitel: | Cytometry Part B: Clinical Cytometry |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , |
| In: | Cytometry Part B: Clinical Cytometry, 74B, 2008, 3, S. 150-155 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Gaipa, Giuseppe Basso, Giuseppe Aliprandi, Sara Migliavacca, Maddalena Vallinoto, Cristina Maglia, Oscar Faini, Andrea Veltroni, Marinella Husak, Dzvenyslava Schumich, Angela Ratei, Richard Biondi, Andrea Dworzak, Michael N. Gaipa, Giuseppe Basso, Giuseppe Aliprandi, Sara Migliavacca, Maddalena Vallinoto, Cristina Maglia, Oscar Faini, Andrea Veltroni, Marinella Husak, Dzvenyslava Schumich, Angela Ratei, Richard Biondi, Andrea Dworzak, Michael N. |
|---|---|
| author |
Gaipa, Giuseppe Basso, Giuseppe Aliprandi, Sara Migliavacca, Maddalena Vallinoto, Cristina Maglia, Oscar Faini, Andrea Veltroni, Marinella Husak, Dzvenyslava Schumich, Angela Ratei, Richard Biondi, Andrea Dworzak, Michael N. |
| spellingShingle |
Gaipa, Giuseppe Basso, Giuseppe Aliprandi, Sara Migliavacca, Maddalena Vallinoto, Cristina Maglia, Oscar Faini, Andrea Veltroni, Marinella Husak, Dzvenyslava Schumich, Angela Ratei, Richard Biondi, Andrea Dworzak, Michael N. Cytometry Part B: Clinical Cytometry Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells Cell Biology Histology Pathology and Forensic Medicine |
| author_sort |
gaipa, giuseppe |
| spelling |
Gaipa, Giuseppe Basso, Giuseppe Aliprandi, Sara Migliavacca, Maddalena Vallinoto, Cristina Maglia, Oscar Faini, Andrea Veltroni, Marinella Husak, Dzvenyslava Schumich, Angela Ratei, Richard Biondi, Andrea Dworzak, Michael N. 1552-4949 1552-4957 Wiley Cell Biology Histology Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.b.20408 <jats:title>Abstract</jats:title><jats:sec><jats:title>Background:</jats:title><jats:p>Immunophenotypic modulation is induced by steroids in pediatric B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) patients during remission induction therapy.</jats:p></jats:sec><jats:sec><jats:title>Methods:</jats:title><jats:p>We cultured BCP‐ALL blasts from diagnostic bone marrow (BM) samples (<jats:italic>n</jats:italic> = 20) in the presence of prednisone on stroma layer obtained from BM‐derived mesenchymal cells to maintain viability. Antigen expression was assessed by multiparametric flow cytometry.</jats:p></jats:sec><jats:sec><jats:title>Results:</jats:title><jats:p>Leukemia samples that sustained the treatment in vitro with prednisone, showed significative reduction of CD10 and CD34 expression compared with control, and it was comparable with that observed in residual leukemic cells of the same patients in BM at day 15 of treatment. Modulated cells were viable as determined by Annexin V staining and preserved light scattering properties. Of note, the extent of antigen modulation in vitro correlated with response to prednisone in vivo.</jats:p></jats:sec><jats:sec><jats:title>Conclusions:</jats:title><jats:p>The prednisone‐induced immunophenotypic modulation can be reproduced in vitro and this phenomenon may reflect sensitivity to chemotherapy. © 2008 Clinical Cytometry Society</jats:p></jats:sec> Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells Cytometry Part B: Clinical Cytometry |
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10.1002/cyto.b.20408 |
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Wiley, 2008 |
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Cytometry Part B: Clinical Cytometry |
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| title |
Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_unstemmed |
Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_full |
Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_fullStr |
Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_full_unstemmed |
Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_short |
Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_sort |
prednisone induces immunophenotypic modulation of cd10 and cd34 in nonapoptotic b‐cell precursor acute lymphoblastic leukemia cells |
| topic |
Cell Biology Histology Pathology and Forensic Medicine |
| url |
http://dx.doi.org/10.1002/cyto.b.20408 |
| publishDate |
2008 |
| physical |
150-155 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background:</jats:title><jats:p>Immunophenotypic modulation is induced by steroids in pediatric B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) patients during remission induction therapy.</jats:p></jats:sec><jats:sec><jats:title>Methods:</jats:title><jats:p>We cultured BCP‐ALL blasts from diagnostic bone marrow (BM) samples (<jats:italic>n</jats:italic> = 20) in the presence of prednisone on stroma layer obtained from BM‐derived mesenchymal cells to maintain viability. Antigen expression was assessed by multiparametric flow cytometry.</jats:p></jats:sec><jats:sec><jats:title>Results:</jats:title><jats:p>Leukemia samples that sustained the treatment in vitro with prednisone, showed significative reduction of CD10 and CD34 expression compared with control, and it was comparable with that observed in residual leukemic cells of the same patients in BM at day 15 of treatment. Modulated cells were viable as determined by Annexin V staining and preserved light scattering properties. Of note, the extent of antigen modulation in vitro correlated with response to prednisone in vivo.</jats:p></jats:sec><jats:sec><jats:title>Conclusions:</jats:title><jats:p>The prednisone‐induced immunophenotypic modulation can be reproduced in vitro and this phenomenon may reflect sensitivity to chemotherapy. © 2008 Clinical Cytometry Society</jats:p></jats:sec> |
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| author | Gaipa, Giuseppe, Basso, Giuseppe, Aliprandi, Sara, Migliavacca, Maddalena, Vallinoto, Cristina, Maglia, Oscar, Faini, Andrea, Veltroni, Marinella, Husak, Dzvenyslava, Schumich, Angela, Ratei, Richard, Biondi, Andrea, Dworzak, Michael N. |
| author_facet | Gaipa, Giuseppe, Basso, Giuseppe, Aliprandi, Sara, Migliavacca, Maddalena, Vallinoto, Cristina, Maglia, Oscar, Faini, Andrea, Veltroni, Marinella, Husak, Dzvenyslava, Schumich, Angela, Ratei, Richard, Biondi, Andrea, Dworzak, Michael N., Gaipa, Giuseppe, Basso, Giuseppe, Aliprandi, Sara, Migliavacca, Maddalena, Vallinoto, Cristina, Maglia, Oscar, Faini, Andrea, Veltroni, Marinella, Husak, Dzvenyslava, Schumich, Angela, Ratei, Richard, Biondi, Andrea, Dworzak, Michael N. |
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| description | <jats:title>Abstract</jats:title><jats:sec><jats:title>Background:</jats:title><jats:p>Immunophenotypic modulation is induced by steroids in pediatric B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) patients during remission induction therapy.</jats:p></jats:sec><jats:sec><jats:title>Methods:</jats:title><jats:p>We cultured BCP‐ALL blasts from diagnostic bone marrow (BM) samples (<jats:italic>n</jats:italic> = 20) in the presence of prednisone on stroma layer obtained from BM‐derived mesenchymal cells to maintain viability. Antigen expression was assessed by multiparametric flow cytometry.</jats:p></jats:sec><jats:sec><jats:title>Results:</jats:title><jats:p>Leukemia samples that sustained the treatment in vitro with prednisone, showed significative reduction of CD10 and CD34 expression compared with control, and it was comparable with that observed in residual leukemic cells of the same patients in BM at day 15 of treatment. Modulated cells were viable as determined by Annexin V staining and preserved light scattering properties. Of note, the extent of antigen modulation in vitro correlated with response to prednisone in vivo.</jats:p></jats:sec><jats:sec><jats:title>Conclusions:</jats:title><jats:p>The prednisone‐induced immunophenotypic modulation can be reproduced in vitro and this phenomenon may reflect sensitivity to chemotherapy. © 2008 Clinical Cytometry Society</jats:p></jats:sec> |
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| imprint | Wiley, 2008 |
| imprint_str_mv | Wiley, 2008 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| spelling | Gaipa, Giuseppe Basso, Giuseppe Aliprandi, Sara Migliavacca, Maddalena Vallinoto, Cristina Maglia, Oscar Faini, Andrea Veltroni, Marinella Husak, Dzvenyslava Schumich, Angela Ratei, Richard Biondi, Andrea Dworzak, Michael N. 1552-4949 1552-4957 Wiley Cell Biology Histology Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.b.20408 <jats:title>Abstract</jats:title><jats:sec><jats:title>Background:</jats:title><jats:p>Immunophenotypic modulation is induced by steroids in pediatric B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) patients during remission induction therapy.</jats:p></jats:sec><jats:sec><jats:title>Methods:</jats:title><jats:p>We cultured BCP‐ALL blasts from diagnostic bone marrow (BM) samples (<jats:italic>n</jats:italic> = 20) in the presence of prednisone on stroma layer obtained from BM‐derived mesenchymal cells to maintain viability. Antigen expression was assessed by multiparametric flow cytometry.</jats:p></jats:sec><jats:sec><jats:title>Results:</jats:title><jats:p>Leukemia samples that sustained the treatment in vitro with prednisone, showed significative reduction of CD10 and CD34 expression compared with control, and it was comparable with that observed in residual leukemic cells of the same patients in BM at day 15 of treatment. Modulated cells were viable as determined by Annexin V staining and preserved light scattering properties. Of note, the extent of antigen modulation in vitro correlated with response to prednisone in vivo.</jats:p></jats:sec><jats:sec><jats:title>Conclusions:</jats:title><jats:p>The prednisone‐induced immunophenotypic modulation can be reproduced in vitro and this phenomenon may reflect sensitivity to chemotherapy. © 2008 Clinical Cytometry Society</jats:p></jats:sec> Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells Cytometry Part B: Clinical Cytometry |
| spellingShingle | Gaipa, Giuseppe, Basso, Giuseppe, Aliprandi, Sara, Migliavacca, Maddalena, Vallinoto, Cristina, Maglia, Oscar, Faini, Andrea, Veltroni, Marinella, Husak, Dzvenyslava, Schumich, Angela, Ratei, Richard, Biondi, Andrea, Dworzak, Michael N., Cytometry Part B: Clinical Cytometry, Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells, Cell Biology, Histology, Pathology and Forensic Medicine |
| title | Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_full | Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_fullStr | Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_full_unstemmed | Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_short | Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| title_sort | prednisone induces immunophenotypic modulation of cd10 and cd34 in nonapoptotic b‐cell precursor acute lymphoblastic leukemia cells |
| title_unstemmed | Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B‐cell precursor acute lymphoblastic leukemia cells |
| topic | Cell Biology, Histology, Pathology and Forensic Medicine |
| url | http://dx.doi.org/10.1002/cyto.b.20408 |