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In vivo imaging of experimental arthritis with near‐infrared fluorescence
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| Zeitschriftentitel: | Arthritis & Rheumatism |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Arthritis & Rheumatism, 50, 2004, 3, S. 961-967 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Hansch, Andreas Frey, Oliver Sauner, Dieter Hilger, Ingrid Haas, Michael Malich, Ansgar Bräuer, Rolf Kaiser, Werner A. Hansch, Andreas Frey, Oliver Sauner, Dieter Hilger, Ingrid Haas, Michael Malich, Ansgar Bräuer, Rolf Kaiser, Werner A. |
|---|---|
| author |
Hansch, Andreas Frey, Oliver Sauner, Dieter Hilger, Ingrid Haas, Michael Malich, Ansgar Bräuer, Rolf Kaiser, Werner A. |
| spellingShingle |
Hansch, Andreas Frey, Oliver Sauner, Dieter Hilger, Ingrid Haas, Michael Malich, Ansgar Bräuer, Rolf Kaiser, Werner A. Arthritis & Rheumatism In vivo imaging of experimental arthritis with near‐infrared fluorescence Pharmacology (medical) Immunology Rheumatology Immunology and Allergy |
| author_sort |
hansch, andreas |
| spelling |
Hansch, Andreas Frey, Oliver Sauner, Dieter Hilger, Ingrid Haas, Michael Malich, Ansgar Bräuer, Rolf Kaiser, Werner A. 0004-3591 1529-0131 Wiley Pharmacology (medical) Immunology Rheumatology Immunology and Allergy http://dx.doi.org/10.1002/art.20112 <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>To visualize early experimental arthritis with near‐infrared fluorescence (NIRF) imaging in a murine model of antigen‐induced arthritis (AIA).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>The target of NIRF was the F4/80 antigen present on the surface of macrophages infiltrating the inflamed synovial membrane. Imaging was performed using anti‐F4/80 monoclonal antibodies (mAb) labeled with Cy5.5 fluorochrome. On day 7 of AIA, 6 mice received an intravenous (IV) injection of labeled mAb; control AIA mice (n = 6) received an IV injection of Cy5.5‐labeled isotype control antibody. NIRF imaging was performed before injection (baseline) and until 72 hours thereafter. Histologic evaluation of arthritis severity and immunohistochemical assessment of F4/80 antigen density were also performed on day 7.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>NIRF imaging showed an accumulation of fluorochrome probes in the inflamed knee joints and, to a lesser extent, in the contralateral (nonarthritic) knee joints. The signal induced by mAb F4/80 was clearly higher than that generated by the isotype control. Accumulation of fluorochrome probes in the joints was confirmed histologically by confocal laser scanning microscopy.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>The use of fluorochromes allows imaging of arthritis in the near‐infrared range. Accumulation in the contralateral, nonarthritic knee joints can be explained by the presence of sentinel macrophages in normal synovium or by a mild contralateral response due to systemic activation or neurogenic mechanisms.</jats:p></jats:sec> In vivo imaging of experimental arthritis with near‐infrared fluorescence Arthritis & Rheumatism |
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10.1002/art.20112 |
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Wiley, 2004 |
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Arthritis & Rheumatism |
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| title |
In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_unstemmed |
In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_full |
In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_fullStr |
In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_full_unstemmed |
In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_short |
In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_sort |
in vivo imaging of experimental arthritis with near‐infrared fluorescence |
| topic |
Pharmacology (medical) Immunology Rheumatology Immunology and Allergy |
| url |
http://dx.doi.org/10.1002/art.20112 |
| publishDate |
2004 |
| physical |
961-967 |
| description |
<jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>To visualize early experimental arthritis with near‐infrared fluorescence (NIRF) imaging in a murine model of antigen‐induced arthritis (AIA).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>The target of NIRF was the F4/80 antigen present on the surface of macrophages infiltrating the inflamed synovial membrane. Imaging was performed using anti‐F4/80 monoclonal antibodies (mAb) labeled with Cy5.5 fluorochrome. On day 7 of AIA, 6 mice received an intravenous (IV) injection of labeled mAb; control AIA mice (n = 6) received an IV injection of Cy5.5‐labeled isotype control antibody. NIRF imaging was performed before injection (baseline) and until 72 hours thereafter. Histologic evaluation of arthritis severity and immunohistochemical assessment of F4/80 antigen density were also performed on day 7.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>NIRF imaging showed an accumulation of fluorochrome probes in the inflamed knee joints and, to a lesser extent, in the contralateral (nonarthritic) knee joints. The signal induced by mAb F4/80 was clearly higher than that generated by the isotype control. Accumulation of fluorochrome probes in the joints was confirmed histologically by confocal laser scanning microscopy.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>The use of fluorochromes allows imaging of arthritis in the near‐infrared range. Accumulation in the contralateral, nonarthritic knee joints can be explained by the presence of sentinel macrophages in normal synovium or by a mild contralateral response due to systemic activation or neurogenic mechanisms.</jats:p></jats:sec> |
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| author | Hansch, Andreas, Frey, Oliver, Sauner, Dieter, Hilger, Ingrid, Haas, Michael, Malich, Ansgar, Bräuer, Rolf, Kaiser, Werner A. |
| author_facet | Hansch, Andreas, Frey, Oliver, Sauner, Dieter, Hilger, Ingrid, Haas, Michael, Malich, Ansgar, Bräuer, Rolf, Kaiser, Werner A., Hansch, Andreas, Frey, Oliver, Sauner, Dieter, Hilger, Ingrid, Haas, Michael, Malich, Ansgar, Bräuer, Rolf, Kaiser, Werner A. |
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| description | <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>To visualize early experimental arthritis with near‐infrared fluorescence (NIRF) imaging in a murine model of antigen‐induced arthritis (AIA).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>The target of NIRF was the F4/80 antigen present on the surface of macrophages infiltrating the inflamed synovial membrane. Imaging was performed using anti‐F4/80 monoclonal antibodies (mAb) labeled with Cy5.5 fluorochrome. On day 7 of AIA, 6 mice received an intravenous (IV) injection of labeled mAb; control AIA mice (n = 6) received an IV injection of Cy5.5‐labeled isotype control antibody. NIRF imaging was performed before injection (baseline) and until 72 hours thereafter. Histologic evaluation of arthritis severity and immunohistochemical assessment of F4/80 antigen density were also performed on day 7.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>NIRF imaging showed an accumulation of fluorochrome probes in the inflamed knee joints and, to a lesser extent, in the contralateral (nonarthritic) knee joints. The signal induced by mAb F4/80 was clearly higher than that generated by the isotype control. Accumulation of fluorochrome probes in the joints was confirmed histologically by confocal laser scanning microscopy.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>The use of fluorochromes allows imaging of arthritis in the near‐infrared range. Accumulation in the contralateral, nonarthritic knee joints can be explained by the presence of sentinel macrophages in normal synovium or by a mild contralateral response due to systemic activation or neurogenic mechanisms.</jats:p></jats:sec> |
| doi_str_mv | 10.1002/art.20112 |
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| imprint | Wiley, 2004 |
| imprint_str_mv | Wiley, 2004 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2 |
| issn | 0004-3591, 1529-0131 |
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| spelling | Hansch, Andreas Frey, Oliver Sauner, Dieter Hilger, Ingrid Haas, Michael Malich, Ansgar Bräuer, Rolf Kaiser, Werner A. 0004-3591 1529-0131 Wiley Pharmacology (medical) Immunology Rheumatology Immunology and Allergy http://dx.doi.org/10.1002/art.20112 <jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>To visualize early experimental arthritis with near‐infrared fluorescence (NIRF) imaging in a murine model of antigen‐induced arthritis (AIA).</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>The target of NIRF was the F4/80 antigen present on the surface of macrophages infiltrating the inflamed synovial membrane. Imaging was performed using anti‐F4/80 monoclonal antibodies (mAb) labeled with Cy5.5 fluorochrome. On day 7 of AIA, 6 mice received an intravenous (IV) injection of labeled mAb; control AIA mice (n = 6) received an IV injection of Cy5.5‐labeled isotype control antibody. NIRF imaging was performed before injection (baseline) and until 72 hours thereafter. Histologic evaluation of arthritis severity and immunohistochemical assessment of F4/80 antigen density were also performed on day 7.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>NIRF imaging showed an accumulation of fluorochrome probes in the inflamed knee joints and, to a lesser extent, in the contralateral (nonarthritic) knee joints. The signal induced by mAb F4/80 was clearly higher than that generated by the isotype control. Accumulation of fluorochrome probes in the joints was confirmed histologically by confocal laser scanning microscopy.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>The use of fluorochromes allows imaging of arthritis in the near‐infrared range. Accumulation in the contralateral, nonarthritic knee joints can be explained by the presence of sentinel macrophages in normal synovium or by a mild contralateral response due to systemic activation or neurogenic mechanisms.</jats:p></jats:sec> In vivo imaging of experimental arthritis with near‐infrared fluorescence Arthritis & Rheumatism |
| spellingShingle | Hansch, Andreas, Frey, Oliver, Sauner, Dieter, Hilger, Ingrid, Haas, Michael, Malich, Ansgar, Bräuer, Rolf, Kaiser, Werner A., Arthritis & Rheumatism, In vivo imaging of experimental arthritis with near‐infrared fluorescence, Pharmacology (medical), Immunology, Rheumatology, Immunology and Allergy |
| title | In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_full | In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_fullStr | In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_full_unstemmed | In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_short | In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_sort | in vivo imaging of experimental arthritis with near‐infrared fluorescence |
| title_unstemmed | In vivo imaging of experimental arthritis with near‐infrared fluorescence |
| topic | Pharmacology (medical), Immunology, Rheumatology, Immunology and Allergy |
| url | http://dx.doi.org/10.1002/art.20112 |