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Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA
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| Zeitschriftentitel: | FEBS Letters |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | FEBS Letters, 591, 2017, 12, S. 1770-1784 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Stelling, Allison L. Xu, Yu Zhou, Huiqing Choi, Seung H. Clay, Mary C. Merriman, Dawn K. Al‐Hashimi, Hashim M. Stelling, Allison L. Xu, Yu Zhou, Huiqing Choi, Seung H. Clay, Mary C. Merriman, Dawn K. Al‐Hashimi, Hashim M. |
|---|---|
| author |
Stelling, Allison L. Xu, Yu Zhou, Huiqing Choi, Seung H. Clay, Mary C. Merriman, Dawn K. Al‐Hashimi, Hashim M. |
| spellingShingle |
Stelling, Allison L. Xu, Yu Zhou, Huiqing Choi, Seung H. Clay, Mary C. Merriman, Dawn K. Al‐Hashimi, Hashim M. FEBS Letters Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
| author_sort |
stelling, allison l. |
| spelling |
Stelling, Allison L. Xu, Yu Zhou, Huiqing Choi, Seung H. Clay, Mary C. Merriman, Dawn K. Al‐Hashimi, Hashim M. 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1002/1873-3468.12681 <jats:p>Noncanonical G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs can form in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> and play roles in recognition, damage repair, and replication. Identifying Hoogsteen base pairs in <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplexes remains challenging due to difficulties in resolving <jats:italic>syn</jats:italic> versus antipurine bases with X‐ray crystallography; and size limitations and line broadening can make them difficult to characterize by <jats:styled-content style="fixed-case">NMR</jats:styled-content> spectroscopy. Here, we show how infrared (<jats:styled-content style="fixed-case">IR</jats:styled-content>) spectroscopy can identify G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> across a range of different structural contexts. The utility of <jats:styled-content style="fixed-case">IR</jats:styled-content>‐based detection of Hoogsteen base pairs is demonstrated by characterizing the first example of adjacent A‐T and G‐C<jats:sup>+</jats:sup> Hoogsteen base pairs in a <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplex where severe broadening complicates detection with <jats:styled-content style="fixed-case">NMR</jats:styled-content>.</jats:p> Robust <scp>IR</scp>‐based detection of stable and fractionally populated G‐C<sup>+</sup> and A‐T Hoogsteen base pairs in duplex <scp>DNA</scp> FEBS Letters |
| doi_str_mv |
10.1002/1873-3468.12681 |
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Biologie Chemie und Pharmazie Physik |
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Wiley, 2017 |
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Wiley, 2017 |
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0014-5793 1873-3468 |
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2017 |
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FEBS Letters |
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49 |
| title |
Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_unstemmed |
Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_full |
Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_fullStr |
Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_full_unstemmed |
Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_short |
Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_sort |
robust <scp>ir</scp>‐based detection of stable and fractionally populated g‐c<sup>+</sup> and a‐t hoogsteen base pairs in duplex <scp>dna</scp> |
| topic |
Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics |
| url |
http://dx.doi.org/10.1002/1873-3468.12681 |
| publishDate |
2017 |
| physical |
1770-1784 |
| description |
<jats:p>Noncanonical G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs can form in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> and play roles in recognition, damage repair, and replication. Identifying Hoogsteen base pairs in <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplexes remains challenging due to difficulties in resolving <jats:italic>syn</jats:italic> versus antipurine bases with X‐ray crystallography; and size limitations and line broadening can make them difficult to characterize by <jats:styled-content style="fixed-case">NMR</jats:styled-content> spectroscopy. Here, we show how infrared (<jats:styled-content style="fixed-case">IR</jats:styled-content>) spectroscopy can identify G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> across a range of different structural contexts. The utility of <jats:styled-content style="fixed-case">IR</jats:styled-content>‐based detection of Hoogsteen base pairs is demonstrated by characterizing the first example of adjacent A‐T and G‐C<jats:sup>+</jats:sup> Hoogsteen base pairs in a <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplex where severe broadening complicates detection with <jats:styled-content style="fixed-case">NMR</jats:styled-content>.</jats:p> |
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| author | Stelling, Allison L., Xu, Yu, Zhou, Huiqing, Choi, Seung H., Clay, Mary C., Merriman, Dawn K., Al‐Hashimi, Hashim M. |
| author_facet | Stelling, Allison L., Xu, Yu, Zhou, Huiqing, Choi, Seung H., Clay, Mary C., Merriman, Dawn K., Al‐Hashimi, Hashim M., Stelling, Allison L., Xu, Yu, Zhou, Huiqing, Choi, Seung H., Clay, Mary C., Merriman, Dawn K., Al‐Hashimi, Hashim M. |
| author_sort | stelling, allison l. |
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| description | <jats:p>Noncanonical G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs can form in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> and play roles in recognition, damage repair, and replication. Identifying Hoogsteen base pairs in <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplexes remains challenging due to difficulties in resolving <jats:italic>syn</jats:italic> versus antipurine bases with X‐ray crystallography; and size limitations and line broadening can make them difficult to characterize by <jats:styled-content style="fixed-case">NMR</jats:styled-content> spectroscopy. Here, we show how infrared (<jats:styled-content style="fixed-case">IR</jats:styled-content>) spectroscopy can identify G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> across a range of different structural contexts. The utility of <jats:styled-content style="fixed-case">IR</jats:styled-content>‐based detection of Hoogsteen base pairs is demonstrated by characterizing the first example of adjacent A‐T and G‐C<jats:sup>+</jats:sup> Hoogsteen base pairs in a <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplex where severe broadening complicates detection with <jats:styled-content style="fixed-case">NMR</jats:styled-content>.</jats:p> |
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| imprint | Wiley, 2017 |
| imprint_str_mv | Wiley, 2017 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105 |
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| mega_collection | Wiley (CrossRef) |
| physical | 1770-1784 |
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| publishDateSort | 2017 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | FEBS Letters |
| source_id | 49 |
| spelling | Stelling, Allison L. Xu, Yu Zhou, Huiqing Choi, Seung H. Clay, Mary C. Merriman, Dawn K. Al‐Hashimi, Hashim M. 0014-5793 1873-3468 Wiley Cell Biology Genetics Molecular Biology Biochemistry Structural Biology Biophysics http://dx.doi.org/10.1002/1873-3468.12681 <jats:p>Noncanonical G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs can form in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> and play roles in recognition, damage repair, and replication. Identifying Hoogsteen base pairs in <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplexes remains challenging due to difficulties in resolving <jats:italic>syn</jats:italic> versus antipurine bases with X‐ray crystallography; and size limitations and line broadening can make them difficult to characterize by <jats:styled-content style="fixed-case">NMR</jats:styled-content> spectroscopy. Here, we show how infrared (<jats:styled-content style="fixed-case">IR</jats:styled-content>) spectroscopy can identify G‐C<jats:sup>+</jats:sup> and A‐T Hoogsteen base pairs in duplex <jats:styled-content style="fixed-case">DNA</jats:styled-content> across a range of different structural contexts. The utility of <jats:styled-content style="fixed-case">IR</jats:styled-content>‐based detection of Hoogsteen base pairs is demonstrated by characterizing the first example of adjacent A‐T and G‐C<jats:sup>+</jats:sup> Hoogsteen base pairs in a <jats:styled-content style="fixed-case">DNA</jats:styled-content> duplex where severe broadening complicates detection with <jats:styled-content style="fixed-case">NMR</jats:styled-content>.</jats:p> Robust <scp>IR</scp>‐based detection of stable and fractionally populated G‐C<sup>+</sup> and A‐T Hoogsteen base pairs in duplex <scp>DNA</scp> FEBS Letters |
| spellingShingle | Stelling, Allison L., Xu, Yu, Zhou, Huiqing, Choi, Seung H., Clay, Mary C., Merriman, Dawn K., Al‐Hashimi, Hashim M., FEBS Letters, Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA, Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| title | Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_full | Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_fullStr | Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_full_unstemmed | Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_short | Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| title_sort | robust <scp>ir</scp>‐based detection of stable and fractionally populated g‐c<sup>+</sup> and a‐t hoogsteen base pairs in duplex <scp>dna</scp> |
| title_unstemmed | Robust IR‐based detection of stable and fractionally populated G‐C+ and A‐T Hoogsteen base pairs in duplex DNA |
| topic | Cell Biology, Genetics, Molecular Biology, Biochemistry, Structural Biology, Biophysics |
| url | http://dx.doi.org/10.1002/1873-3468.12681 |