Eintrag weiter verarbeiten
Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients
Gespeichert in:
| Zeitschriftentitel: | Molecular Oncology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , , , , , , , , , , |
| In: | Molecular Oncology, 14, 2020, 5, S. 1001-1015 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Schneegans, Svenja Lück, Lelia Besler, Katharina Bluhm, Leonie Stadler, Julia‐Christina Staub, Janina Greinert, Rüdiger Volkmer, Beate Kubista, Mikael Gebhardt, Christoffer Sartori, Alexander Irwin, Darryl Serkkola, Elina af Hällström, Taija Lianidou, Evi Sprenger‐Haussels, Markus Hussong, Melanie Mohr, Peter Schneider, Stefan W. Shaffer, Jonathan Pantel, Klaus Wikman, Harriet Schneegans, Svenja Lück, Lelia Besler, Katharina Bluhm, Leonie Stadler, Julia‐Christina Staub, Janina Greinert, Rüdiger Volkmer, Beate Kubista, Mikael Gebhardt, Christoffer Sartori, Alexander Irwin, Darryl Serkkola, Elina af Hällström, Taija Lianidou, Evi Sprenger‐Haussels, Markus Hussong, Melanie Mohr, Peter Schneider, Stefan W. Shaffer, Jonathan Pantel, Klaus Wikman, Harriet |
|---|---|
| author |
Schneegans, Svenja Lück, Lelia Besler, Katharina Bluhm, Leonie Stadler, Julia‐Christina Staub, Janina Greinert, Rüdiger Volkmer, Beate Kubista, Mikael Gebhardt, Christoffer Sartori, Alexander Irwin, Darryl Serkkola, Elina af Hällström, Taija Lianidou, Evi Sprenger‐Haussels, Markus Hussong, Melanie Mohr, Peter Schneider, Stefan W. Shaffer, Jonathan Pantel, Klaus Wikman, Harriet |
| spellingShingle |
Schneegans, Svenja Lück, Lelia Besler, Katharina Bluhm, Leonie Stadler, Julia‐Christina Staub, Janina Greinert, Rüdiger Volkmer, Beate Kubista, Mikael Gebhardt, Christoffer Sartori, Alexander Irwin, Darryl Serkkola, Elina af Hällström, Taija Lianidou, Evi Sprenger‐Haussels, Markus Hussong, Melanie Mohr, Peter Schneider, Stefan W. Shaffer, Jonathan Pantel, Klaus Wikman, Harriet Molecular Oncology Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients Cancer Research Genetics Molecular Medicine General Medicine Oncology |
| author_sort |
schneegans, svenja |
| spelling |
Schneegans, Svenja Lück, Lelia Besler, Katharina Bluhm, Leonie Stadler, Julia‐Christina Staub, Janina Greinert, Rüdiger Volkmer, Beate Kubista, Mikael Gebhardt, Christoffer Sartori, Alexander Irwin, Darryl Serkkola, Elina af Hällström, Taija Lianidou, Evi Sprenger‐Haussels, Markus Hussong, Melanie Mohr, Peter Schneider, Stefan W. Shaffer, Jonathan Pantel, Klaus Wikman, Harriet 1574-7891 1878-0261 Wiley Cancer Research Genetics Molecular Medicine General Medicine Oncology http://dx.doi.org/10.1002/1878-0261.12669 <jats:p>The combination of liquid biomarkers from a single blood tube can provide more comprehensive information on tumor development and progression in cancer patients compared to single analysis. Here, we evaluated whether a combined analysis of circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and circulating cell‐free microRNA (miRNA) in total plasma and extracellular vesicles (EV) from the same blood sample is feasible and how the results are influenced by the choice of different blood tubes. Peripheral blood from 20 stage IV melanoma patients and five healthy donors (HD) was collected in EDTA, Streck, and Transfix tubes. Peripheral blood mononuclear cell fraction was used for CTC analysis, whereas plasma and EV fractions were used for ctDNA mutation and miRNA analysis. Mutations in cell‐free circulating DNA were detected in 67% of patients, with no significant difference between the tubes. CTC was detected in only EDTA blood and only in 15% of patients. miRNA NGS (next‐generation sequencing) results were highly influenced by the collection tubes and could only be performed from EDTA and Streck tubes due to hemolysis in Transfix tubes. No overlap of significantly differentially expressed miRNA (patients versus HD) could be found between the tubes in total plasma, whereas eight miRNA were commonly differentially regulated in the EV fraction. In summary, high‐quality CTCs, ctDNA, and miRNA data from a single blood tube can be obtained. However, the choice of blood collection tubes is a critical pre‐analytical variable.</jats:p> Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients Molecular Oncology |
| doi_str_mv |
10.1002/1878-0261.12669 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi8xODc4LTAyNjEuMTI2Njk |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi8xODc4LTAyNjEuMTI2Njk |
| institution |
DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 |
| imprint |
Wiley, 2020 |
| imprint_str_mv |
Wiley, 2020 |
| issn |
1574-7891 1878-0261 |
| issn_str_mv |
1574-7891 1878-0261 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
schneegans2020preanalyticalfactorsaffectingtheestablishmentofasingletubeassayformultiparameterliquidbiopsydetectioninmelanomapatients |
| publishDateSort |
2020 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
Molecular Oncology |
| source_id |
49 |
| title |
Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_unstemmed |
Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_full |
Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_fullStr |
Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_full_unstemmed |
Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_short |
Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_sort |
pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| topic |
Cancer Research Genetics Molecular Medicine General Medicine Oncology |
| url |
http://dx.doi.org/10.1002/1878-0261.12669 |
| publishDate |
2020 |
| physical |
1001-1015 |
| description |
<jats:p>The combination of liquid biomarkers from a single blood tube can provide more comprehensive information on tumor development and progression in cancer patients compared to single analysis. Here, we evaluated whether a combined analysis of circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and circulating cell‐free microRNA (miRNA) in total plasma and extracellular vesicles (EV) from the same blood sample is feasible and how the results are influenced by the choice of different blood tubes. Peripheral blood from 20 stage IV melanoma patients and five healthy donors (HD) was collected in EDTA, Streck, and Transfix tubes. Peripheral blood mononuclear cell fraction was used for CTC analysis, whereas plasma and EV fractions were used for ctDNA mutation and miRNA analysis. Mutations in cell‐free circulating DNA were detected in 67% of patients, with no significant difference between the tubes. CTC was detected in only EDTA blood and only in 15% of patients. miRNA NGS (next‐generation sequencing) results were highly influenced by the collection tubes and could only be performed from EDTA and Streck tubes due to hemolysis in Transfix tubes. No overlap of significantly differentially expressed miRNA (patients versus HD) could be found between the tubes in total plasma, whereas eight miRNA were commonly differentially regulated in the EV fraction. In summary, high‐quality CTCs, ctDNA, and miRNA data from a single blood tube can be obtained. However, the choice of blood collection tubes is a critical pre‐analytical variable.</jats:p> |
| container_issue |
5 |
| container_start_page |
1001 |
| container_title |
Molecular Oncology |
| container_volume |
14 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792345545848651778 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:24:27.441Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Pre%E2%80%90analytical+factors+affecting+the+establishment+of+a+single+tube+assay+for+multiparameter+liquid+biopsy+detection+in+melanoma+patients&rft.date=2020-05-01&genre=article&issn=1878-0261&volume=14&issue=5&spage=1001&epage=1015&pages=1001-1015&jtitle=Molecular+Oncology&atitle=Pre%E2%80%90analytical+factors+affecting+the+establishment+of+a+single+tube+assay+for+multiparameter+liquid+biopsy+detection+in+melanoma+patients&aulast=Wikman&aufirst=Harriet&rft_id=info%3Adoi%2F10.1002%2F1878-0261.12669&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792345545848651778 |
| author | Schneegans, Svenja, Lück, Lelia, Besler, Katharina, Bluhm, Leonie, Stadler, Julia‐Christina, Staub, Janina, Greinert, Rüdiger, Volkmer, Beate, Kubista, Mikael, Gebhardt, Christoffer, Sartori, Alexander, Irwin, Darryl, Serkkola, Elina, af Hällström, Taija, Lianidou, Evi, Sprenger‐Haussels, Markus, Hussong, Melanie, Mohr, Peter, Schneider, Stefan W., Shaffer, Jonathan, Pantel, Klaus, Wikman, Harriet |
| author_facet | Schneegans, Svenja, Lück, Lelia, Besler, Katharina, Bluhm, Leonie, Stadler, Julia‐Christina, Staub, Janina, Greinert, Rüdiger, Volkmer, Beate, Kubista, Mikael, Gebhardt, Christoffer, Sartori, Alexander, Irwin, Darryl, Serkkola, Elina, af Hällström, Taija, Lianidou, Evi, Sprenger‐Haussels, Markus, Hussong, Melanie, Mohr, Peter, Schneider, Stefan W., Shaffer, Jonathan, Pantel, Klaus, Wikman, Harriet, Schneegans, Svenja, Lück, Lelia, Besler, Katharina, Bluhm, Leonie, Stadler, Julia‐Christina, Staub, Janina, Greinert, Rüdiger, Volkmer, Beate, Kubista, Mikael, Gebhardt, Christoffer, Sartori, Alexander, Irwin, Darryl, Serkkola, Elina, af Hällström, Taija, Lianidou, Evi, Sprenger‐Haussels, Markus, Hussong, Melanie, Mohr, Peter, Schneider, Stefan W., Shaffer, Jonathan, Pantel, Klaus, Wikman, Harriet |
| author_sort | schneegans, svenja |
| container_issue | 5 |
| container_start_page | 1001 |
| container_title | Molecular Oncology |
| container_volume | 14 |
| description | <jats:p>The combination of liquid biomarkers from a single blood tube can provide more comprehensive information on tumor development and progression in cancer patients compared to single analysis. Here, we evaluated whether a combined analysis of circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and circulating cell‐free microRNA (miRNA) in total plasma and extracellular vesicles (EV) from the same blood sample is feasible and how the results are influenced by the choice of different blood tubes. Peripheral blood from 20 stage IV melanoma patients and five healthy donors (HD) was collected in EDTA, Streck, and Transfix tubes. Peripheral blood mononuclear cell fraction was used for CTC analysis, whereas plasma and EV fractions were used for ctDNA mutation and miRNA analysis. Mutations in cell‐free circulating DNA were detected in 67% of patients, with no significant difference between the tubes. CTC was detected in only EDTA blood and only in 15% of patients. miRNA NGS (next‐generation sequencing) results were highly influenced by the collection tubes and could only be performed from EDTA and Streck tubes due to hemolysis in Transfix tubes. No overlap of significantly differentially expressed miRNA (patients versus HD) could be found between the tubes in total plasma, whereas eight miRNA were commonly differentially regulated in the EV fraction. In summary, high‐quality CTCs, ctDNA, and miRNA data from a single blood tube can be obtained. However, the choice of blood collection tubes is a critical pre‐analytical variable.</jats:p> |
| doi_str_mv | 10.1002/1878-0261.12669 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin, Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi8xODc4LTAyNjEuMTI2Njk |
| imprint | Wiley, 2020 |
| imprint_str_mv | Wiley, 2020 |
| institution | DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3 |
| issn | 1574-7891, 1878-0261 |
| issn_str_mv | 1574-7891, 1878-0261 |
| language | English |
| last_indexed | 2024-03-01T17:24:27.441Z |
| match_str | schneegans2020preanalyticalfactorsaffectingtheestablishmentofasingletubeassayformultiparameterliquidbiopsydetectioninmelanomapatients |
| mega_collection | Wiley (CrossRef) |
| physical | 1001-1015 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | Molecular Oncology |
| source_id | 49 |
| spelling | Schneegans, Svenja Lück, Lelia Besler, Katharina Bluhm, Leonie Stadler, Julia‐Christina Staub, Janina Greinert, Rüdiger Volkmer, Beate Kubista, Mikael Gebhardt, Christoffer Sartori, Alexander Irwin, Darryl Serkkola, Elina af Hällström, Taija Lianidou, Evi Sprenger‐Haussels, Markus Hussong, Melanie Mohr, Peter Schneider, Stefan W. Shaffer, Jonathan Pantel, Klaus Wikman, Harriet 1574-7891 1878-0261 Wiley Cancer Research Genetics Molecular Medicine General Medicine Oncology http://dx.doi.org/10.1002/1878-0261.12669 <jats:p>The combination of liquid biomarkers from a single blood tube can provide more comprehensive information on tumor development and progression in cancer patients compared to single analysis. Here, we evaluated whether a combined analysis of circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and circulating cell‐free microRNA (miRNA) in total plasma and extracellular vesicles (EV) from the same blood sample is feasible and how the results are influenced by the choice of different blood tubes. Peripheral blood from 20 stage IV melanoma patients and five healthy donors (HD) was collected in EDTA, Streck, and Transfix tubes. Peripheral blood mononuclear cell fraction was used for CTC analysis, whereas plasma and EV fractions were used for ctDNA mutation and miRNA analysis. Mutations in cell‐free circulating DNA were detected in 67% of patients, with no significant difference between the tubes. CTC was detected in only EDTA blood and only in 15% of patients. miRNA NGS (next‐generation sequencing) results were highly influenced by the collection tubes and could only be performed from EDTA and Streck tubes due to hemolysis in Transfix tubes. No overlap of significantly differentially expressed miRNA (patients versus HD) could be found between the tubes in total plasma, whereas eight miRNA were commonly differentially regulated in the EV fraction. In summary, high‐quality CTCs, ctDNA, and miRNA data from a single blood tube can be obtained. However, the choice of blood collection tubes is a critical pre‐analytical variable.</jats:p> Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients Molecular Oncology |
| spellingShingle | Schneegans, Svenja, Lück, Lelia, Besler, Katharina, Bluhm, Leonie, Stadler, Julia‐Christina, Staub, Janina, Greinert, Rüdiger, Volkmer, Beate, Kubista, Mikael, Gebhardt, Christoffer, Sartori, Alexander, Irwin, Darryl, Serkkola, Elina, af Hällström, Taija, Lianidou, Evi, Sprenger‐Haussels, Markus, Hussong, Melanie, Mohr, Peter, Schneider, Stefan W., Shaffer, Jonathan, Pantel, Klaus, Wikman, Harriet, Molecular Oncology, Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients, Cancer Research, Genetics, Molecular Medicine, General Medicine, Oncology |
| title | Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_full | Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_fullStr | Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_full_unstemmed | Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_short | Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_sort | pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| title_unstemmed | Pre‐analytical factors affecting the establishment of a single tube assay for multiparameter liquid biopsy detection in melanoma patients |
| topic | Cancer Research, Genetics, Molecular Medicine, General Medicine, Oncology |
| url | http://dx.doi.org/10.1002/1878-0261.12669 |