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l-Serine, d- and l-proline and alanine as respiratory substrates of Helicobacter pylori: correlation between in vitro and in vivo amino acid levels
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| Zeitschriftentitel: | Microbiology |
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| Personen und Körperschaften: | , , , , , |
| In: | Microbiology, 149, 2003, 8, S. 2023-2030 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Microbiology Society
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| Schlagwörter: |
| Zusammenfassung: | <jats:p><jats:italic>Helicobacter pylori</jats:italic>whole cells showed high rates of oxygen uptake with<jats:sc>l</jats:sc>-serine and<jats:sc>l</jats:sc>-proline as respiratory substrates, and somewhat lower rates with<jats:sc>d</jats:sc>-alanine and<jats:sc>d</jats:sc>-proline. These respiratory activities were inhibited by rotenone and antimycin A at low concentrations. Since pyruvate was produced from<jats:sc>l</jats:sc>-serine and<jats:sc>d</jats:sc>- and<jats:sc>l</jats:sc>-alanine in whole cells, the respiratory activities with these amino acids as substrates occurred via pyruvate. Whole cells showed 2,6-dichlorophenolindophenol (DCIP)-reducing activities with<jats:sc>d</jats:sc>- and<jats:sc>l</jats:sc>-proline and<jats:sc>d</jats:sc>-alanine as substrates, suggesting that hydrogen removed from these amino acids also participated in oxygen uptake by the whole cells. High amounts of<jats:sc>l</jats:sc>-proline,<jats:sc>d</jats:sc>- and<jats:sc>l</jats:sc>-alanine, and<jats:sc>l</jats:sc>-serine were present in<jats:italic>H. pylori</jats:italic>cells, and these amino acids also predominated in samples of human gastric juice.<jats:italic>H. pylori</jats:italic>seems to utilize<jats:sc>d</jats:sc>- and<jats:sc>l</jats:sc>-proline,<jats:sc>d</jats:sc>-alanine and<jats:sc>l</jats:sc>-serine as important energy sources in its habitat of the mucous layer of the stomach.</jats:p> |
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| Umfang: | 2023-2030 |
| ISSN: |
1350-0872
1465-2080 |
| DOI: | 10.1099/mic.0.26203-0 |