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High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma
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| Zeitschriftentitel: | Journal of General Virology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , , , , |
| In: | Journal of General Virology, 92, 2011, 6, S. 1343-1351 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Microbiology Society
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| Schlagwörter: |
| author_facet |
Dalagiorgou, G. Vassilaki, N. Foka, P. Boumlic, A. Kakkanas, A. Kochlios, E. Khalili, S. Aslanoglou, E. Veletza, S. Orfanoudakis, G. Vassilopoulos, D. Hadziyannis, S. J. Koskinas, J. Mavromara, P. Dalagiorgou, G. Vassilaki, N. Foka, P. Boumlic, A. Kakkanas, A. Kochlios, E. Khalili, S. Aslanoglou, E. Veletza, S. Orfanoudakis, G. Vassilopoulos, D. Hadziyannis, S. J. Koskinas, J. Mavromara, P. |
|---|---|
| author |
Dalagiorgou, G. Vassilaki, N. Foka, P. Boumlic, A. Kakkanas, A. Kochlios, E. Khalili, S. Aslanoglou, E. Veletza, S. Orfanoudakis, G. Vassilopoulos, D. Hadziyannis, S. J. Koskinas, J. Mavromara, P. |
| spellingShingle |
Dalagiorgou, G. Vassilaki, N. Foka, P. Boumlic, A. Kakkanas, A. Kochlios, E. Khalili, S. Aslanoglou, E. Veletza, S. Orfanoudakis, G. Vassilopoulos, D. Hadziyannis, S. J. Koskinas, J. Mavromara, P. Journal of General Virology High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma Virology |
| author_sort |
dalagiorgou, g. |
| spelling |
Dalagiorgou, G. Vassilaki, N. Foka, P. Boumlic, A. Kakkanas, A. Kochlios, E. Khalili, S. Aslanoglou, E. Veletza, S. Orfanoudakis, G. Vassilopoulos, D. Hadziyannis, S. J. Koskinas, J. Mavromara, P. 0022-1317 1465-2099 Microbiology Society Virology http://dx.doi.org/10.1099/vir.0.023010-0 <jats:p>The core region of the hepatitis C virus (HCV) genome possesses an overlapping ORF that has been shown to encode a protein, known as the alternate reading frame protein (ARFP), F or core+1. The biological role of this protein remains elusive, as it appears to be non-essential for virus replication. However, a number of independent studies have shown that the ARFP/F/core+1 protein elicits humoral and cellular immune responses in HCV-infected individuals and interacts with important cellular proteins. To assess the significance of the core+1 humoral response in HCV-infected patients, we examined the prevalence of anti-core+1 antibodies in sera from patients with hepatocellular carcinoma (HCC) in comparison with chronically HCV-infected individuals without HCC. We produced two HCV core+1 histidine-tagged recombinant proteins for genotypes 1a (aa 11–160) and 1b (aa 11–144), as well as a non-tagged highly purified recombinant core+1/S protein (aa 85–144) of HCV-1b. Using an in-house ELISA, we tested the prevalence of core+1 antibodies in 45 patients with HCC in comparison with 47 chronically HCV-infected patients without HCC and 77 negative-control sera. More than 50 % of the serum samples from HCC patients reacted with all core+1 antigens, whereas <26 % of the sera from the non-HCC HCV-infected individuals tested positive. No core+1-specific reactivity was detected in any of the control samples. In conclusion, the high occurrence of anti-core+1 antibodies in the serum of HCC patients suggests a role for the ARFP/F/core+1 protein in the pathogenesis of HCC.</jats:p> High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma Journal of General Virology |
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| title |
High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_unstemmed |
High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_full |
High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_fullStr |
High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_full_unstemmed |
High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_short |
High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_sort |
high levels of hcv core+1 antibodies in hcv patients with hepatocellular carcinoma |
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Virology |
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http://dx.doi.org/10.1099/vir.0.023010-0 |
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2011 |
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1343-1351 |
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<jats:p>The core region of the hepatitis C virus (HCV) genome possesses an overlapping ORF that has been shown to encode a protein, known as the alternate reading frame protein (ARFP), F or core+1. The biological role of this protein remains elusive, as it appears to be non-essential for virus replication. However, a number of independent studies have shown that the ARFP/F/core+1 protein elicits humoral and cellular immune responses in HCV-infected individuals and interacts with important cellular proteins. To assess the significance of the core+1 humoral response in HCV-infected patients, we examined the prevalence of anti-core+1 antibodies in sera from patients with hepatocellular carcinoma (HCC) in comparison with chronically HCV-infected individuals without HCC. We produced two HCV core+1 histidine-tagged recombinant proteins for genotypes 1a (aa 11–160) and 1b (aa 11–144), as well as a non-tagged highly purified recombinant core+1/S protein (aa 85–144) of HCV-1b. Using an in-house ELISA, we tested the prevalence of core+1 antibodies in 45 patients with HCC in comparison with 47 chronically HCV-infected patients without HCC and 77 negative-control sera. More than 50 % of the serum samples from HCC patients reacted with all core+1 antigens, whereas <26 % of the sera from the non-HCC HCV-infected individuals tested positive. No core+1-specific reactivity was detected in any of the control samples. In conclusion, the high occurrence of anti-core+1 antibodies in the serum of HCC patients suggests a role for the ARFP/F/core+1 protein in the pathogenesis of HCC.</jats:p> |
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| author | Dalagiorgou, G., Vassilaki, N., Foka, P., Boumlic, A., Kakkanas, A., Kochlios, E., Khalili, S., Aslanoglou, E., Veletza, S., Orfanoudakis, G., Vassilopoulos, D., Hadziyannis, S. J., Koskinas, J., Mavromara, P. |
| author_facet | Dalagiorgou, G., Vassilaki, N., Foka, P., Boumlic, A., Kakkanas, A., Kochlios, E., Khalili, S., Aslanoglou, E., Veletza, S., Orfanoudakis, G., Vassilopoulos, D., Hadziyannis, S. J., Koskinas, J., Mavromara, P., Dalagiorgou, G., Vassilaki, N., Foka, P., Boumlic, A., Kakkanas, A., Kochlios, E., Khalili, S., Aslanoglou, E., Veletza, S., Orfanoudakis, G., Vassilopoulos, D., Hadziyannis, S. J., Koskinas, J., Mavromara, P. |
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| description | <jats:p>The core region of the hepatitis C virus (HCV) genome possesses an overlapping ORF that has been shown to encode a protein, known as the alternate reading frame protein (ARFP), F or core+1. The biological role of this protein remains elusive, as it appears to be non-essential for virus replication. However, a number of independent studies have shown that the ARFP/F/core+1 protein elicits humoral and cellular immune responses in HCV-infected individuals and interacts with important cellular proteins. To assess the significance of the core+1 humoral response in HCV-infected patients, we examined the prevalence of anti-core+1 antibodies in sera from patients with hepatocellular carcinoma (HCC) in comparison with chronically HCV-infected individuals without HCC. We produced two HCV core+1 histidine-tagged recombinant proteins for genotypes 1a (aa 11–160) and 1b (aa 11–144), as well as a non-tagged highly purified recombinant core+1/S protein (aa 85–144) of HCV-1b. Using an in-house ELISA, we tested the prevalence of core+1 antibodies in 45 patients with HCC in comparison with 47 chronically HCV-infected patients without HCC and 77 negative-control sera. More than 50 % of the serum samples from HCC patients reacted with all core+1 antigens, whereas <26 % of the sera from the non-HCC HCV-infected individuals tested positive. No core+1-specific reactivity was detected in any of the control samples. In conclusion, the high occurrence of anti-core+1 antibodies in the serum of HCC patients suggests a role for the ARFP/F/core+1 protein in the pathogenesis of HCC.</jats:p> |
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| spelling | Dalagiorgou, G. Vassilaki, N. Foka, P. Boumlic, A. Kakkanas, A. Kochlios, E. Khalili, S. Aslanoglou, E. Veletza, S. Orfanoudakis, G. Vassilopoulos, D. Hadziyannis, S. J. Koskinas, J. Mavromara, P. 0022-1317 1465-2099 Microbiology Society Virology http://dx.doi.org/10.1099/vir.0.023010-0 <jats:p>The core region of the hepatitis C virus (HCV) genome possesses an overlapping ORF that has been shown to encode a protein, known as the alternate reading frame protein (ARFP), F or core+1. The biological role of this protein remains elusive, as it appears to be non-essential for virus replication. However, a number of independent studies have shown that the ARFP/F/core+1 protein elicits humoral and cellular immune responses in HCV-infected individuals and interacts with important cellular proteins. To assess the significance of the core+1 humoral response in HCV-infected patients, we examined the prevalence of anti-core+1 antibodies in sera from patients with hepatocellular carcinoma (HCC) in comparison with chronically HCV-infected individuals without HCC. We produced two HCV core+1 histidine-tagged recombinant proteins for genotypes 1a (aa 11–160) and 1b (aa 11–144), as well as a non-tagged highly purified recombinant core+1/S protein (aa 85–144) of HCV-1b. Using an in-house ELISA, we tested the prevalence of core+1 antibodies in 45 patients with HCC in comparison with 47 chronically HCV-infected patients without HCC and 77 negative-control sera. More than 50 % of the serum samples from HCC patients reacted with all core+1 antigens, whereas <26 % of the sera from the non-HCC HCV-infected individuals tested positive. No core+1-specific reactivity was detected in any of the control samples. In conclusion, the high occurrence of anti-core+1 antibodies in the serum of HCC patients suggests a role for the ARFP/F/core+1 protein in the pathogenesis of HCC.</jats:p> High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma Journal of General Virology |
| spellingShingle | Dalagiorgou, G., Vassilaki, N., Foka, P., Boumlic, A., Kakkanas, A., Kochlios, E., Khalili, S., Aslanoglou, E., Veletza, S., Orfanoudakis, G., Vassilopoulos, D., Hadziyannis, S. J., Koskinas, J., Mavromara, P., Journal of General Virology, High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma, Virology |
| title | High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_full | High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_fullStr | High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_full_unstemmed | High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_short | High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| title_sort | high levels of hcv core+1 antibodies in hcv patients with hepatocellular carcinoma |
| title_unstemmed | High levels of HCV core+1 antibodies in HCV patients with hepatocellular carcinoma |
| topic | Virology |
| url | http://dx.doi.org/10.1099/vir.0.023010-0 |