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Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection
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| Zeitschriftentitel: | Journal of General Virology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Journal of General Virology, 90, 2009, 7, S. 1692-1701 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Microbiology Society
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| Schlagwörter: |
| author_facet |
Billerbeck, Eva Nakamoto, Nobuhiro Seigel, Bianca Blum, Hubert E. Chang, Kyong-Mi Thimme, Robert Billerbeck, Eva Nakamoto, Nobuhiro Seigel, Bianca Blum, Hubert E. Chang, Kyong-Mi Thimme, Robert |
|---|---|
| author |
Billerbeck, Eva Nakamoto, Nobuhiro Seigel, Bianca Blum, Hubert E. Chang, Kyong-Mi Thimme, Robert |
| spellingShingle |
Billerbeck, Eva Nakamoto, Nobuhiro Seigel, Bianca Blum, Hubert E. Chang, Kyong-Mi Thimme, Robert Journal of General Virology Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection Virology |
| author_sort |
billerbeck, eva |
| spelling |
Billerbeck, Eva Nakamoto, Nobuhiro Seigel, Bianca Blum, Hubert E. Chang, Kyong-Mi Thimme, Robert 0022-1317 1465-2099 Microbiology Society Virology http://dx.doi.org/10.1099/vir.0.009837-0 <jats:p>It has been shown previously that suppressive virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells can be expanded from human peripheral blood mononuclear cells after <jats:italic>in vitro</jats:italic> antigen-specific stimulation. This study extended this finding by analysing the mechanisms of virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T-cell generation during peptide-specific expansion <jats:italic>in vitro</jats:italic>. It was shown that hepatitis C virus (HCV)-, influenza virus (FLU)-, Epstein–Barr virus (EBV)- and cytomegalovirus (HCMV)-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells could be expanded differentially from the blood of chronically HCV-infected patients following <jats:italic>in vitro</jats:italic> peptide-specific stimulation. The different ability of virus-specific CD8<jats:sup>+</jats:sup> T-cell populations to express FoxP3 after continuous antigen stimulation <jats:italic>in vitro</jats:italic> correlated significantly with the <jats:italic>ex vivo</jats:italic> differentiation status. Indeed, CD27<jats:sup>+</jats:sup> CD28<jats:sup>+</jats:sup> CD57<jats:sup>−</jats:sup> HCV-, FLU- and EBV-specific CD8<jats:sup>+</jats:sup> T cells displayed a significantly higher ability to give rise to FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells compared with CD27<jats:sup>−</jats:sup> CD28<jats:sup>−</jats:sup> CD57<jats:sup>+</jats:sup> HCMV-specific CD8<jats:sup>+</jats:sup> T cells. Similar T-cell receptor expression patterns of FoxP3<jats:sup>+</jats:sup> versus FoxP3<jats:sup>−</jats:sup> CD8<jats:sup>+</jats:sup> T cells of the same antigen specificity indicated that both cell populations were probably expanded from the same virus-specific CD8<jats:sup>+</jats:sup> T-cell precursor. In addition, no specific antigen-presenting cell populations were required for the generation of FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells, as CD8<jats:sup>+</jats:sup>-selected virus-specific FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells could be expanded by peptide presentation in the absence of antigen-presenting cells. Taken together, these results suggest that the ability to expand FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells from virus-specific CD8<jats:sup>+</jats:sup> T cells differs among distinct virus-specific CD8<jats:sup>+</jats:sup> T-cell populations depending on the differentiation status.</jats:p> Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection Journal of General Virology |
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10.1099/vir.0.009837-0 |
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Microbiology Society, 2009 |
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Microbiology Society, 2009 |
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billerbeck2009determinantsofinvitroexpansionofdifferenthumanvirusspecificfoxp3regulatorycd8tcellsinchronichepatitiscvirusinfection |
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2009 |
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Microbiology Society |
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ai |
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ai |
| series |
Journal of General Virology |
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49 |
| title |
Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_unstemmed |
Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_full |
Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_fullStr |
Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_full_unstemmed |
Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_short |
Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_sort |
determinants of in vitro expansion of different human virus-specific foxp3+ regulatory cd8+ t cells in chronic hepatitis c virus infection |
| topic |
Virology |
| url |
http://dx.doi.org/10.1099/vir.0.009837-0 |
| publishDate |
2009 |
| physical |
1692-1701 |
| description |
<jats:p>It has been shown previously that suppressive virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells can be expanded from human peripheral blood mononuclear cells after <jats:italic>in vitro</jats:italic> antigen-specific stimulation. This study extended this finding by analysing the mechanisms of virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T-cell generation during peptide-specific expansion <jats:italic>in vitro</jats:italic>. It was shown that hepatitis C virus (HCV)-, influenza virus (FLU)-, Epstein–Barr virus (EBV)- and cytomegalovirus (HCMV)-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells could be expanded differentially from the blood of chronically HCV-infected patients following <jats:italic>in vitro</jats:italic> peptide-specific stimulation. The different ability of virus-specific CD8<jats:sup>+</jats:sup> T-cell populations to express FoxP3 after continuous antigen stimulation <jats:italic>in vitro</jats:italic> correlated significantly with the <jats:italic>ex vivo</jats:italic> differentiation status. Indeed, CD27<jats:sup>+</jats:sup> CD28<jats:sup>+</jats:sup> CD57<jats:sup>−</jats:sup> HCV-, FLU- and EBV-specific CD8<jats:sup>+</jats:sup> T cells displayed a significantly higher ability to give rise to FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells compared with CD27<jats:sup>−</jats:sup> CD28<jats:sup>−</jats:sup> CD57<jats:sup>+</jats:sup> HCMV-specific CD8<jats:sup>+</jats:sup> T cells. Similar T-cell receptor expression patterns of FoxP3<jats:sup>+</jats:sup> versus FoxP3<jats:sup>−</jats:sup> CD8<jats:sup>+</jats:sup> T cells of the same antigen specificity indicated that both cell populations were probably expanded from the same virus-specific CD8<jats:sup>+</jats:sup> T-cell precursor. In addition, no specific antigen-presenting cell populations were required for the generation of FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells, as CD8<jats:sup>+</jats:sup>-selected virus-specific FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells could be expanded by peptide presentation in the absence of antigen-presenting cells. Taken together, these results suggest that the ability to expand FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells from virus-specific CD8<jats:sup>+</jats:sup> T cells differs among distinct virus-specific CD8<jats:sup>+</jats:sup> T-cell populations depending on the differentiation status.</jats:p> |
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| author | Billerbeck, Eva, Nakamoto, Nobuhiro, Seigel, Bianca, Blum, Hubert E., Chang, Kyong-Mi, Thimme, Robert |
| author_facet | Billerbeck, Eva, Nakamoto, Nobuhiro, Seigel, Bianca, Blum, Hubert E., Chang, Kyong-Mi, Thimme, Robert, Billerbeck, Eva, Nakamoto, Nobuhiro, Seigel, Bianca, Blum, Hubert E., Chang, Kyong-Mi, Thimme, Robert |
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| description | <jats:p>It has been shown previously that suppressive virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells can be expanded from human peripheral blood mononuclear cells after <jats:italic>in vitro</jats:italic> antigen-specific stimulation. This study extended this finding by analysing the mechanisms of virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T-cell generation during peptide-specific expansion <jats:italic>in vitro</jats:italic>. It was shown that hepatitis C virus (HCV)-, influenza virus (FLU)-, Epstein–Barr virus (EBV)- and cytomegalovirus (HCMV)-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells could be expanded differentially from the blood of chronically HCV-infected patients following <jats:italic>in vitro</jats:italic> peptide-specific stimulation. The different ability of virus-specific CD8<jats:sup>+</jats:sup> T-cell populations to express FoxP3 after continuous antigen stimulation <jats:italic>in vitro</jats:italic> correlated significantly with the <jats:italic>ex vivo</jats:italic> differentiation status. Indeed, CD27<jats:sup>+</jats:sup> CD28<jats:sup>+</jats:sup> CD57<jats:sup>−</jats:sup> HCV-, FLU- and EBV-specific CD8<jats:sup>+</jats:sup> T cells displayed a significantly higher ability to give rise to FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells compared with CD27<jats:sup>−</jats:sup> CD28<jats:sup>−</jats:sup> CD57<jats:sup>+</jats:sup> HCMV-specific CD8<jats:sup>+</jats:sup> T cells. Similar T-cell receptor expression patterns of FoxP3<jats:sup>+</jats:sup> versus FoxP3<jats:sup>−</jats:sup> CD8<jats:sup>+</jats:sup> T cells of the same antigen specificity indicated that both cell populations were probably expanded from the same virus-specific CD8<jats:sup>+</jats:sup> T-cell precursor. In addition, no specific antigen-presenting cell populations were required for the generation of FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells, as CD8<jats:sup>+</jats:sup>-selected virus-specific FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells could be expanded by peptide presentation in the absence of antigen-presenting cells. Taken together, these results suggest that the ability to expand FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells from virus-specific CD8<jats:sup>+</jats:sup> T cells differs among distinct virus-specific CD8<jats:sup>+</jats:sup> T-cell populations depending on the differentiation status.</jats:p> |
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| spelling | Billerbeck, Eva Nakamoto, Nobuhiro Seigel, Bianca Blum, Hubert E. Chang, Kyong-Mi Thimme, Robert 0022-1317 1465-2099 Microbiology Society Virology http://dx.doi.org/10.1099/vir.0.009837-0 <jats:p>It has been shown previously that suppressive virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells can be expanded from human peripheral blood mononuclear cells after <jats:italic>in vitro</jats:italic> antigen-specific stimulation. This study extended this finding by analysing the mechanisms of virus-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T-cell generation during peptide-specific expansion <jats:italic>in vitro</jats:italic>. It was shown that hepatitis C virus (HCV)-, influenza virus (FLU)-, Epstein–Barr virus (EBV)- and cytomegalovirus (HCMV)-specific FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells could be expanded differentially from the blood of chronically HCV-infected patients following <jats:italic>in vitro</jats:italic> peptide-specific stimulation. The different ability of virus-specific CD8<jats:sup>+</jats:sup> T-cell populations to express FoxP3 after continuous antigen stimulation <jats:italic>in vitro</jats:italic> correlated significantly with the <jats:italic>ex vivo</jats:italic> differentiation status. Indeed, CD27<jats:sup>+</jats:sup> CD28<jats:sup>+</jats:sup> CD57<jats:sup>−</jats:sup> HCV-, FLU- and EBV-specific CD8<jats:sup>+</jats:sup> T cells displayed a significantly higher ability to give rise to FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells compared with CD27<jats:sup>−</jats:sup> CD28<jats:sup>−</jats:sup> CD57<jats:sup>+</jats:sup> HCMV-specific CD8<jats:sup>+</jats:sup> T cells. Similar T-cell receptor expression patterns of FoxP3<jats:sup>+</jats:sup> versus FoxP3<jats:sup>−</jats:sup> CD8<jats:sup>+</jats:sup> T cells of the same antigen specificity indicated that both cell populations were probably expanded from the same virus-specific CD8<jats:sup>+</jats:sup> T-cell precursor. In addition, no specific antigen-presenting cell populations were required for the generation of FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells, as CD8<jats:sup>+</jats:sup>-selected virus-specific FoxP3<jats:sup>+</jats:sup> CD8<jats:sup>+</jats:sup> T cells could be expanded by peptide presentation in the absence of antigen-presenting cells. Taken together, these results suggest that the ability to expand FoxP3<jats:sup>+</jats:sup> regulatory CD8<jats:sup>+</jats:sup> T cells from virus-specific CD8<jats:sup>+</jats:sup> T cells differs among distinct virus-specific CD8<jats:sup>+</jats:sup> T-cell populations depending on the differentiation status.</jats:p> Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection Journal of General Virology |
| spellingShingle | Billerbeck, Eva, Nakamoto, Nobuhiro, Seigel, Bianca, Blum, Hubert E., Chang, Kyong-Mi, Thimme, Robert, Journal of General Virology, Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection, Virology |
| title | Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_full | Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_fullStr | Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_full_unstemmed | Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_short | Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| title_sort | determinants of in vitro expansion of different human virus-specific foxp3+ regulatory cd8+ t cells in chronic hepatitis c virus infection |
| title_unstemmed | Determinants of in vitro expansion of different human virus-specific FoxP3+ regulatory CD8+ T cells in chronic hepatitis C virus infection |
| topic | Virology |
| url | http://dx.doi.org/10.1099/vir.0.009837-0 |