Eintrag weiter verarbeiten
Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus
Gespeichert in:
| Zeitschriftentitel: | Journal of General Virology |
|---|---|
| Personen und Körperschaften: | , , , , , , , , , , |
| In: | Journal of General Virology, 87, 2006, 6, S. 1691-1695 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Microbiology Society
|
| Schlagwörter: |
| author_facet |
Ren, Xiaofeng Glende, Jörg Al-Falah, Marwan de Vries, Victor Schwegmann-Wessels, Christel Qu, Xiuxia Tan, Lei Tschernig, Thomas Deng, Hongkui Naim, Hassan Y. Herrler, Georg Ren, Xiaofeng Glende, Jörg Al-Falah, Marwan de Vries, Victor Schwegmann-Wessels, Christel Qu, Xiuxia Tan, Lei Tschernig, Thomas Deng, Hongkui Naim, Hassan Y. Herrler, Georg |
|---|---|
| author |
Ren, Xiaofeng Glende, Jörg Al-Falah, Marwan de Vries, Victor Schwegmann-Wessels, Christel Qu, Xiuxia Tan, Lei Tschernig, Thomas Deng, Hongkui Naim, Hassan Y. Herrler, Georg |
| spellingShingle |
Ren, Xiaofeng Glende, Jörg Al-Falah, Marwan de Vries, Victor Schwegmann-Wessels, Christel Qu, Xiuxia Tan, Lei Tschernig, Thomas Deng, Hongkui Naim, Hassan Y. Herrler, Georg Journal of General Virology Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus Virology |
| author_sort |
ren, xiaofeng |
| spelling |
Ren, Xiaofeng Glende, Jörg Al-Falah, Marwan de Vries, Victor Schwegmann-Wessels, Christel Qu, Xiuxia Tan, Lei Tschernig, Thomas Deng, Hongkui Naim, Hassan Y. Herrler, Georg 0022-1317 1465-2099 Microbiology Society Virology http://dx.doi.org/10.1099/vir.0.81749-0 <jats:p>The primary target of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is epithelial cells in the respiratory and intestinal tract. The cellular receptor for SARS-CoV, angiotensin-converting enzyme 2 (ACE2), has been shown to be localized on the apical plasma membrane of polarized respiratory epithelial cells and to mediate infection from the apical side of these cells. Here, these results were confirmed and extended by including a colon carcinoma cell line (Caco-2), a lung carcinoma cell line (Calu-3) and Vero E6 cells in our analysis. All three cell types expressed human ACE2 on the apical membrane domain and were infected via this route, as determined with vesicular stomatitis virus pseudotypes containing the S protein of SARS-CoV. In a histological analysis of the respiratory tract, ACE2 was detected in the trachea, main bronchus and alveoli, and occasionally also in the small bronchi. These data will help us to understand the pathogenesis of SARS-CoV infection.</jats:p> Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus Journal of General Virology |
| doi_str_mv |
10.1099/vir.0.81749-0 |
| facet_avail |
Online Free |
| finc_class_facet |
Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5OS92aXIuMC44MTc0OS0w |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5OS92aXIuMC44MTc0OS0w |
| institution |
DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Rs1 DE-Pl11 DE-105 DE-14 DE-Ch1 DE-L229 |
| imprint |
Microbiology Society, 2006 |
| imprint_str_mv |
Microbiology Society, 2006 |
| issn |
0022-1317 1465-2099 |
| issn_str_mv |
0022-1317 1465-2099 |
| language |
English |
| mega_collection |
Microbiology Society (CrossRef) |
| match_str |
ren2006analysisoface2inpolarizedepithelialcellssurfaceexpressionandfunctionasreceptorforsevereacuterespiratorysyndromeassociatedcoronavirus |
| publishDateSort |
2006 |
| publisher |
Microbiology Society |
| recordtype |
ai |
| record_format |
ai |
| series |
Journal of General Virology |
| source_id |
49 |
| title |
Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_unstemmed |
Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_full |
Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_fullStr |
Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_full_unstemmed |
Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_short |
Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_sort |
analysis of ace2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| topic |
Virology |
| url |
http://dx.doi.org/10.1099/vir.0.81749-0 |
| publishDate |
2006 |
| physical |
1691-1695 |
| description |
<jats:p>The primary target of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is epithelial cells in the respiratory and intestinal tract. The cellular receptor for SARS-CoV, angiotensin-converting enzyme 2 (ACE2), has been shown to be localized on the apical plasma membrane of polarized respiratory epithelial cells and to mediate infection from the apical side of these cells. Here, these results were confirmed and extended by including a colon carcinoma cell line (Caco-2), a lung carcinoma cell line (Calu-3) and Vero E6 cells in our analysis. All three cell types expressed human ACE2 on the apical membrane domain and were infected via this route, as determined with vesicular stomatitis virus pseudotypes containing the S protein of SARS-CoV. In a histological analysis of the respiratory tract, ACE2 was detected in the trachea, main bronchus and alveoli, and occasionally also in the small bronchi. These data will help us to understand the pathogenesis of SARS-CoV infection.</jats:p> |
| container_issue |
6 |
| container_start_page |
1691 |
| container_title |
Journal of General Virology |
| container_volume |
87 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792343125796061185 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:46:44.681Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Analysis+of+ACE2+in+polarized+epithelial+cells%3A+surface+expression+and+function+as+receptor+for+severe+acute+respiratory+syndrome-associated+coronavirus&rft.date=2006-06-01&genre=article&issn=1465-2099&volume=87&issue=6&spage=1691&epage=1695&pages=1691-1695&jtitle=Journal+of+General+Virology&atitle=Analysis+of+ACE2+in+polarized+epithelial+cells%3A+surface+expression+and+function+as+receptor+for+severe+acute+respiratory+syndrome-associated+coronavirus&aulast=Herrler&aufirst=Georg&rft_id=info%3Adoi%2F10.1099%2Fvir.0.81749-0&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792343125796061185 |
| author | Ren, Xiaofeng, Glende, Jörg, Al-Falah, Marwan, de Vries, Victor, Schwegmann-Wessels, Christel, Qu, Xiuxia, Tan, Lei, Tschernig, Thomas, Deng, Hongkui, Naim, Hassan Y., Herrler, Georg |
| author_facet | Ren, Xiaofeng, Glende, Jörg, Al-Falah, Marwan, de Vries, Victor, Schwegmann-Wessels, Christel, Qu, Xiuxia, Tan, Lei, Tschernig, Thomas, Deng, Hongkui, Naim, Hassan Y., Herrler, Georg, Ren, Xiaofeng, Glende, Jörg, Al-Falah, Marwan, de Vries, Victor, Schwegmann-Wessels, Christel, Qu, Xiuxia, Tan, Lei, Tschernig, Thomas, Deng, Hongkui, Naim, Hassan Y., Herrler, Georg |
| author_sort | ren, xiaofeng |
| container_issue | 6 |
| container_start_page | 1691 |
| container_title | Journal of General Virology |
| container_volume | 87 |
| description | <jats:p>The primary target of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is epithelial cells in the respiratory and intestinal tract. The cellular receptor for SARS-CoV, angiotensin-converting enzyme 2 (ACE2), has been shown to be localized on the apical plasma membrane of polarized respiratory epithelial cells and to mediate infection from the apical side of these cells. Here, these results were confirmed and extended by including a colon carcinoma cell line (Caco-2), a lung carcinoma cell line (Calu-3) and Vero E6 cells in our analysis. All three cell types expressed human ACE2 on the apical membrane domain and were infected via this route, as determined with vesicular stomatitis virus pseudotypes containing the S protein of SARS-CoV. In a histological analysis of the respiratory tract, ACE2 was detected in the trachea, main bronchus and alveoli, and occasionally also in the small bronchi. These data will help us to understand the pathogenesis of SARS-CoV infection.</jats:p> |
| doi_str_mv | 10.1099/vir.0.81749-0 |
| facet_avail | Online, Free |
| finc_class_facet | Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5OS92aXIuMC44MTc0OS0w |
| imprint | Microbiology Society, 2006 |
| imprint_str_mv | Microbiology Society, 2006 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
| issn | 0022-1317, 1465-2099 |
| issn_str_mv | 0022-1317, 1465-2099 |
| language | English |
| last_indexed | 2024-03-01T16:46:44.681Z |
| match_str | ren2006analysisoface2inpolarizedepithelialcellssurfaceexpressionandfunctionasreceptorforsevereacuterespiratorysyndromeassociatedcoronavirus |
| mega_collection | Microbiology Society (CrossRef) |
| physical | 1691-1695 |
| publishDate | 2006 |
| publishDateSort | 2006 |
| publisher | Microbiology Society |
| record_format | ai |
| recordtype | ai |
| series | Journal of General Virology |
| source_id | 49 |
| spelling | Ren, Xiaofeng Glende, Jörg Al-Falah, Marwan de Vries, Victor Schwegmann-Wessels, Christel Qu, Xiuxia Tan, Lei Tschernig, Thomas Deng, Hongkui Naim, Hassan Y. Herrler, Georg 0022-1317 1465-2099 Microbiology Society Virology http://dx.doi.org/10.1099/vir.0.81749-0 <jats:p>The primary target of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is epithelial cells in the respiratory and intestinal tract. The cellular receptor for SARS-CoV, angiotensin-converting enzyme 2 (ACE2), has been shown to be localized on the apical plasma membrane of polarized respiratory epithelial cells and to mediate infection from the apical side of these cells. Here, these results were confirmed and extended by including a colon carcinoma cell line (Caco-2), a lung carcinoma cell line (Calu-3) and Vero E6 cells in our analysis. All three cell types expressed human ACE2 on the apical membrane domain and were infected via this route, as determined with vesicular stomatitis virus pseudotypes containing the S protein of SARS-CoV. In a histological analysis of the respiratory tract, ACE2 was detected in the trachea, main bronchus and alveoli, and occasionally also in the small bronchi. These data will help us to understand the pathogenesis of SARS-CoV infection.</jats:p> Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus Journal of General Virology |
| spellingShingle | Ren, Xiaofeng, Glende, Jörg, Al-Falah, Marwan, de Vries, Victor, Schwegmann-Wessels, Christel, Qu, Xiuxia, Tan, Lei, Tschernig, Thomas, Deng, Hongkui, Naim, Hassan Y., Herrler, Georg, Journal of General Virology, Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus, Virology |
| title | Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_full | Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_fullStr | Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_full_unstemmed | Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_short | Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_sort | analysis of ace2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| title_unstemmed | Analysis of ACE2 in polarized epithelial cells: surface expression and function as receptor for severe acute respiratory syndrome-associated coronavirus |
| topic | Virology |
| url | http://dx.doi.org/10.1099/vir.0.81749-0 |