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Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice
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| Zeitschriftentitel: | Open Biology |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | Open Biology, 7, 2017, 1, S. 160303 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
The Royal Society
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| Schlagwörter: |
| author_facet |
Liu, Ting-Yuan Chen, Yu-Chia Jong, Yuh-Jyh Tsai, Huai-Jen Lee, Chien-Chin Chang, Ya-Sian Chang, Jan-Gowth Chang, Yung-Fu Liu, Ting-Yuan Chen, Yu-Chia Jong, Yuh-Jyh Tsai, Huai-Jen Lee, Chien-Chin Chang, Ya-Sian Chang, Jan-Gowth Chang, Yung-Fu |
|---|---|
| author |
Liu, Ting-Yuan Chen, Yu-Chia Jong, Yuh-Jyh Tsai, Huai-Jen Lee, Chien-Chin Chang, Ya-Sian Chang, Jan-Gowth Chang, Yung-Fu |
| spellingShingle |
Liu, Ting-Yuan Chen, Yu-Chia Jong, Yuh-Jyh Tsai, Huai-Jen Lee, Chien-Chin Chang, Ya-Sian Chang, Jan-Gowth Chang, Yung-Fu Open Biology Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice General Biochemistry, Genetics and Molecular Biology Immunology General Neuroscience |
| author_sort |
liu, ting-yuan |
| spelling |
Liu, Ting-Yuan Chen, Yu-Chia Jong, Yuh-Jyh Tsai, Huai-Jen Lee, Chien-Chin Chang, Ya-Sian Chang, Jan-Gowth Chang, Yung-Fu 2046-2441 The Royal Society General Biochemistry, Genetics and Molecular Biology Immunology General Neuroscience http://dx.doi.org/10.1098/rsob.160303 <jats:p> Heterogeneous ribonucleoprotein A1 (hnRNP A1) is crucial for regulating alternative splicing. Its integrated function within an organism has not, however, been identified. We generated hnRNP A1 knockout mice to study the role of hnRNP A1 <jats:italic>in vivo</jats:italic> . The knockout mice, <jats:italic>hnRNP A1</jats:italic> <jats:sup>−/−</jats:sup> , showed embryonic lethality because of muscle developmental defects. The blood pressure and heart rate of the heterozygous mice were higher than those of the wild-type mice, indicating heart function defects. We performed mouse exon arrays to study the muscle development mechanism. The processes regulated by hnRNP A1 included cell adhesion and muscle contraction. The expression levels of muscle development-related genes in <jats:italic>hnRNP A1</jats:italic> <jats:sup>+/−</jats:sup> mice were significantly different from those in wild-type mice, as detected using qRT-PCR. We further confirmed the alternative splicing patterns of muscle development-related genes including <jats:italic>mef2c</jats:italic> , <jats:italic>lrrfip1</jats:italic> , <jats:italic>usp28</jats:italic> and <jats:italic>abcc9</jats:italic> . Alternative mRNA isoforms of these genes were increased in <jats:italic>hnRNP A1</jats:italic> <jats:sup>+/−</jats:sup> mice compared with wild-type mice. Furthermore, we revealed that the functionally similar hnRNP A2/B1 did not compensate for the expression of hnRNP A1 in organisms. In summary, our study demonstrated that hnRNP A1 plays a critical and irreplaceable role in embryonic muscle development by regulating the expression and alternative splicing of muscle-related genes. </jats:p> Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice Open Biology |
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| title |
Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_unstemmed |
Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_full |
Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_fullStr |
Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_full_unstemmed |
Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_short |
Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_sort |
muscle developmental defects in heterogeneous nuclear ribonucleoprotein a1 knockout mice |
| topic |
General Biochemistry, Genetics and Molecular Biology Immunology General Neuroscience |
| url |
http://dx.doi.org/10.1098/rsob.160303 |
| publishDate |
2017 |
| physical |
160303 |
| description |
<jats:p>
Heterogeneous ribonucleoprotein A1 (hnRNP A1) is crucial for regulating alternative splicing. Its integrated function within an organism has not, however, been identified. We generated hnRNP A1 knockout mice to study the role of hnRNP A1
<jats:italic>in vivo</jats:italic>
. The knockout mice,
<jats:italic>hnRNP A1</jats:italic>
<jats:sup>−/−</jats:sup>
, showed embryonic lethality because of muscle developmental defects. The blood pressure and heart rate of the heterozygous mice were higher than those of the wild-type mice, indicating heart function defects. We performed mouse exon arrays to study the muscle development mechanism. The processes regulated by hnRNP A1 included cell adhesion and muscle contraction. The expression levels of muscle development-related genes in
<jats:italic>hnRNP A1</jats:italic>
<jats:sup>+/−</jats:sup>
mice were significantly different from those in wild-type mice, as detected using qRT-PCR. We further confirmed the alternative splicing patterns of muscle development-related genes including
<jats:italic>mef2c</jats:italic>
,
<jats:italic>lrrfip1</jats:italic>
,
<jats:italic>usp28</jats:italic>
and
<jats:italic>abcc9</jats:italic>
. Alternative mRNA isoforms of these genes were increased in
<jats:italic>hnRNP A1</jats:italic>
<jats:sup>+/−</jats:sup>
mice compared with wild-type mice. Furthermore, we revealed that the functionally similar hnRNP A2/B1 did not compensate for the expression of hnRNP A1 in organisms. In summary, our study demonstrated that hnRNP A1 plays a critical and irreplaceable role in embryonic muscle development by regulating the expression and alternative splicing of muscle-related genes.
</jats:p> |
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| author | Liu, Ting-Yuan, Chen, Yu-Chia, Jong, Yuh-Jyh, Tsai, Huai-Jen, Lee, Chien-Chin, Chang, Ya-Sian, Chang, Jan-Gowth, Chang, Yung-Fu |
| author_facet | Liu, Ting-Yuan, Chen, Yu-Chia, Jong, Yuh-Jyh, Tsai, Huai-Jen, Lee, Chien-Chin, Chang, Ya-Sian, Chang, Jan-Gowth, Chang, Yung-Fu, Liu, Ting-Yuan, Chen, Yu-Chia, Jong, Yuh-Jyh, Tsai, Huai-Jen, Lee, Chien-Chin, Chang, Ya-Sian, Chang, Jan-Gowth, Chang, Yung-Fu |
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| description | <jats:p> Heterogeneous ribonucleoprotein A1 (hnRNP A1) is crucial for regulating alternative splicing. Its integrated function within an organism has not, however, been identified. We generated hnRNP A1 knockout mice to study the role of hnRNP A1 <jats:italic>in vivo</jats:italic> . The knockout mice, <jats:italic>hnRNP A1</jats:italic> <jats:sup>−/−</jats:sup> , showed embryonic lethality because of muscle developmental defects. The blood pressure and heart rate of the heterozygous mice were higher than those of the wild-type mice, indicating heart function defects. We performed mouse exon arrays to study the muscle development mechanism. The processes regulated by hnRNP A1 included cell adhesion and muscle contraction. The expression levels of muscle development-related genes in <jats:italic>hnRNP A1</jats:italic> <jats:sup>+/−</jats:sup> mice were significantly different from those in wild-type mice, as detected using qRT-PCR. We further confirmed the alternative splicing patterns of muscle development-related genes including <jats:italic>mef2c</jats:italic> , <jats:italic>lrrfip1</jats:italic> , <jats:italic>usp28</jats:italic> and <jats:italic>abcc9</jats:italic> . Alternative mRNA isoforms of these genes were increased in <jats:italic>hnRNP A1</jats:italic> <jats:sup>+/−</jats:sup> mice compared with wild-type mice. Furthermore, we revealed that the functionally similar hnRNP A2/B1 did not compensate for the expression of hnRNP A1 in organisms. In summary, our study demonstrated that hnRNP A1 plays a critical and irreplaceable role in embryonic muscle development by regulating the expression and alternative splicing of muscle-related genes. </jats:p> |
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| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| spelling | Liu, Ting-Yuan Chen, Yu-Chia Jong, Yuh-Jyh Tsai, Huai-Jen Lee, Chien-Chin Chang, Ya-Sian Chang, Jan-Gowth Chang, Yung-Fu 2046-2441 The Royal Society General Biochemistry, Genetics and Molecular Biology Immunology General Neuroscience http://dx.doi.org/10.1098/rsob.160303 <jats:p> Heterogeneous ribonucleoprotein A1 (hnRNP A1) is crucial for regulating alternative splicing. Its integrated function within an organism has not, however, been identified. We generated hnRNP A1 knockout mice to study the role of hnRNP A1 <jats:italic>in vivo</jats:italic> . The knockout mice, <jats:italic>hnRNP A1</jats:italic> <jats:sup>−/−</jats:sup> , showed embryonic lethality because of muscle developmental defects. The blood pressure and heart rate of the heterozygous mice were higher than those of the wild-type mice, indicating heart function defects. We performed mouse exon arrays to study the muscle development mechanism. The processes regulated by hnRNP A1 included cell adhesion and muscle contraction. The expression levels of muscle development-related genes in <jats:italic>hnRNP A1</jats:italic> <jats:sup>+/−</jats:sup> mice were significantly different from those in wild-type mice, as detected using qRT-PCR. We further confirmed the alternative splicing patterns of muscle development-related genes including <jats:italic>mef2c</jats:italic> , <jats:italic>lrrfip1</jats:italic> , <jats:italic>usp28</jats:italic> and <jats:italic>abcc9</jats:italic> . Alternative mRNA isoforms of these genes were increased in <jats:italic>hnRNP A1</jats:italic> <jats:sup>+/−</jats:sup> mice compared with wild-type mice. Furthermore, we revealed that the functionally similar hnRNP A2/B1 did not compensate for the expression of hnRNP A1 in organisms. In summary, our study demonstrated that hnRNP A1 plays a critical and irreplaceable role in embryonic muscle development by regulating the expression and alternative splicing of muscle-related genes. </jats:p> Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice Open Biology |
| spellingShingle | Liu, Ting-Yuan, Chen, Yu-Chia, Jong, Yuh-Jyh, Tsai, Huai-Jen, Lee, Chien-Chin, Chang, Ya-Sian, Chang, Jan-Gowth, Chang, Yung-Fu, Open Biology, Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice, General Biochemistry, Genetics and Molecular Biology, Immunology, General Neuroscience |
| title | Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_full | Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_fullStr | Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_full_unstemmed | Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_short | Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| title_sort | muscle developmental defects in heterogeneous nuclear ribonucleoprotein a1 knockout mice |
| title_unstemmed | Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice |
| topic | General Biochemistry, Genetics and Molecular Biology, Immunology, General Neuroscience |
| url | http://dx.doi.org/10.1098/rsob.160303 |