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Global Changes in Gene Expression in Sinorhizobium meliloti 1021 under Microoxic and Symbiotic Conditions
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| Zeitschriftentitel: | Molecular Plant-Microbe Interactions® |
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| Personen und Körperschaften: | , , , , , , , , , , , , , , , , , |
| In: | Molecular Plant-Microbe Interactions®, 17, 2004, 3, S. 292-303 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Scientific Societies
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| Zusammenfassung: | <jats:p> Sinorhizobium meliloti is an α-proteobacterium that alternates between a free-living phase in bulk soil or in the rhizosphere of plants and a symbiotic phase within the host plant cells, where the bacteria ultimately differentiate into nitrogen-fixing organelle-like cells, called bacteroids. As a step toward understanding the physiology of S. meliloti in its free-living and symbiotic forms and the transition between the two, gene expression profiles were determined under two sets of biological conditions: growth under oxic versus microoxic conditions, and in free-living versus symbiotic state. Data acquisition was based on both macro- and microarrays. Transcriptome profiles highlighted a profound modification of gene expression during bacteroid differentiation, with 16% of genes being altered. The data are consistent with an overall slow down of bacteroid metabolism during adaptation to symbiotic life and acquisition of nitrogen fixation capability. A large number of genes of unknown function, including potential regulators, that may play a role in symbiosis were identified. Transcriptome profiling in response to oxygen limitation indicated that up to 5% of the genes were oxygen regulated. However, the microoxic and bacteroid transcriptomes only partially overlap, implying that oxygen contributes to a limited extent to the control of symbiotic gene expression. </jats:p> |
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| Umfang: | 292-303 |
| ISSN: |
0894-0282
1943-7706 |
| DOI: | 10.1094/mpmi.2004.17.3.292 |