Eintrag weiter verarbeiten
Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test
Gespeichert in:
Zeitschriftentitel: | Open Forum Infectious Diseases |
---|---|
Personen und Körperschaften: | , , , , , |
In: | Open Forum Infectious Diseases, 2, 2015, 2 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Oxford University Press (OUP)
|
Schlagwörter: |
author_facet |
Kokuto, Hiroyuki Sasaki, Yuka Yoshimatsu, Shoji Mizuno, Kazue Yi, Lina Mitarai, Satoshi Kokuto, Hiroyuki Sasaki, Yuka Yoshimatsu, Shoji Mizuno, Kazue Yi, Lina Mitarai, Satoshi |
---|---|
author |
Kokuto, Hiroyuki Sasaki, Yuka Yoshimatsu, Shoji Mizuno, Kazue Yi, Lina Mitarai, Satoshi |
spellingShingle |
Kokuto, Hiroyuki Sasaki, Yuka Yoshimatsu, Shoji Mizuno, Kazue Yi, Lina Mitarai, Satoshi Open Forum Infectious Diseases Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test Infectious Diseases Oncology |
author_sort |
kokuto, hiroyuki |
spelling |
Kokuto, Hiroyuki Sasaki, Yuka Yoshimatsu, Shoji Mizuno, Kazue Yi, Lina Mitarai, Satoshi 2328-8957 Oxford University Press (OUP) Infectious Diseases Oncology http://dx.doi.org/10.1093/ofid/ofv074 <jats:title>Abstract</jats:title> <jats:p>Background. The Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) is a fully automated diagnostic test that allows for the detection of MTB including its RIF resistance. Although the test is used for the diagnosis of tuberculosis (TB) in sputum samples worldwide, studies using fecal specimens are scarce. We therefore evaluated the efficacy of the Xpert MTB/RIF test for detection of MTB in fecal specimens obtained from adult pulmonary TB patients, confirmed by culture and/or molecular diagnostic methods.</jats:p> <jats:p>Methods. We conducted a retrospective case-control study to provide proof-of-concept regarding the efficacy of the Xpert MTB/RIF test using fecal samples for diagnosing pulmonary TB via detection of MTB in adult patients (≥20 years) at the Fukujuji Hospital in Tokyo, Japan.</jats:p> <jats:p>Results. Fecal specimens were obtained from 56 active pulmonary TB patients (including 48 sputum smear-positive and 8 sputum smear-negative patients), 10 non-TB patients (including 4 Myocobacterium avium complex infections), and 27 healthy individuals who were exposed to active pulmonary TB patients. The sensitivity of the fecal Xpert MTB/RIF was 100% (81.7%–100%) for detection of MTB in specimens from sputum smear-positive (1+ to 3+) patients, 81.0% (58.1%–94.6%) in specimens from sputum smear scanty positive patients, and 50.0% (15.7%–84.3%) in specimens from sputum smear-negative patients. Meanwhile, each of the fecal specimens from the non-TB group was negative for MTB (specificity 100%; 95% confidence interval, 86.2–100).</jats:p> <jats:p>Conclusions. The fecal Xpert MTB/RIF test could detect MTB in a large proportion of smear-positive pulmonary TB patients, without frequent false-positive results at a TB referral hospital in Japan.</jats:p> Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test Open Forum Infectious Diseases |
doi_str_mv |
10.1093/ofid/ofv074 |
facet_avail |
Online Free |
finc_class_facet |
Medizin |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9vZmlkL29mdjA3NA |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9vZmlkL29mdjA3NA |
institution |
DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 |
imprint |
Oxford University Press (OUP), 2015 |
imprint_str_mv |
Oxford University Press (OUP), 2015 |
issn |
2328-8957 |
issn_str_mv |
2328-8957 |
language |
English |
mega_collection |
Oxford University Press (OUP) (CrossRef) |
match_str |
kokuto2015detectionofmycobacteriumtuberculosismtbinfecalspecimensfromadultsdiagnosedwithpulmonarytuberculosisusingthexpertmtbrifampicintest |
publishDateSort |
2015 |
publisher |
Oxford University Press (OUP) |
recordtype |
ai |
record_format |
ai |
series |
Open Forum Infectious Diseases |
source_id |
49 |
title |
Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_unstemmed |
Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_full |
Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_fullStr |
Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_full_unstemmed |
Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_short |
Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_sort |
detection of mycobacterium tuberculosis (mtb) in fecal specimens from adults diagnosed with pulmonary tuberculosis using the xpert mtb/rifampicin test |
topic |
Infectious Diseases Oncology |
url |
http://dx.doi.org/10.1093/ofid/ofv074 |
publishDate |
2015 |
physical |
|
description |
<jats:title>Abstract</jats:title>
<jats:p>Background. The Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) is a fully automated diagnostic test that allows for the detection of MTB including its RIF resistance. Although the test is used for the diagnosis of tuberculosis (TB) in sputum samples worldwide, studies using fecal specimens are scarce. We therefore evaluated the efficacy of the Xpert MTB/RIF test for detection of MTB in fecal specimens obtained from adult pulmonary TB patients, confirmed by culture and/or molecular diagnostic methods.</jats:p>
<jats:p>Methods. We conducted a retrospective case-control study to provide proof-of-concept regarding the efficacy of the Xpert MTB/RIF test using fecal samples for diagnosing pulmonary TB via detection of MTB in adult patients (≥20 years) at the Fukujuji Hospital in Tokyo, Japan.</jats:p>
<jats:p>Results. Fecal specimens were obtained from 56 active pulmonary TB patients (including 48 sputum smear-positive and 8 sputum smear-negative patients), 10 non-TB patients (including 4 Myocobacterium avium complex infections), and 27 healthy individuals who were exposed to active pulmonary TB patients. The sensitivity of the fecal Xpert MTB/RIF was 100% (81.7%–100%) for detection of MTB in specimens from sputum smear-positive (1+ to 3+) patients, 81.0% (58.1%–94.6%) in specimens from sputum smear scanty positive patients, and 50.0% (15.7%–84.3%) in specimens from sputum smear-negative patients. Meanwhile, each of the fecal specimens from the non-TB group was negative for MTB (specificity 100%; 95% confidence interval, 86.2–100).</jats:p>
<jats:p>Conclusions. The fecal Xpert MTB/RIF test could detect MTB in a large proportion of smear-positive pulmonary TB patients, without frequent false-positive results at a TB referral hospital in Japan.</jats:p> |
container_issue |
2 |
container_start_page |
0 |
container_title |
Open Forum Infectious Diseases |
container_volume |
2 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792344896637501450 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T17:14:49.954Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Detection+of+Mycobacterium+tuberculosis+%28MTB%29+in+Fecal+Specimens+From+Adults+Diagnosed+With+Pulmonary+Tuberculosis+Using+the+Xpert+MTB%2FRifampicin+Test&rft.date=2015-04-01&genre=article&issn=2328-8957&volume=2&issue=2&jtitle=Open+Forum+Infectious+Diseases&atitle=Detection+of+Mycobacterium+tuberculosis+%28MTB%29+in+Fecal+Specimens+From+Adults+Diagnosed+With+Pulmonary+Tuberculosis+Using+the+Xpert+MTB%2FRifampicin+Test&aulast=Mitarai&aufirst=Satoshi&rft_id=info%3Adoi%2F10.1093%2Fofid%2Fofv074&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792344896637501450 |
author | Kokuto, Hiroyuki, Sasaki, Yuka, Yoshimatsu, Shoji, Mizuno, Kazue, Yi, Lina, Mitarai, Satoshi |
author_facet | Kokuto, Hiroyuki, Sasaki, Yuka, Yoshimatsu, Shoji, Mizuno, Kazue, Yi, Lina, Mitarai, Satoshi, Kokuto, Hiroyuki, Sasaki, Yuka, Yoshimatsu, Shoji, Mizuno, Kazue, Yi, Lina, Mitarai, Satoshi |
author_sort | kokuto, hiroyuki |
container_issue | 2 |
container_start_page | 0 |
container_title | Open Forum Infectious Diseases |
container_volume | 2 |
description | <jats:title>Abstract</jats:title> <jats:p>Background. The Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) is a fully automated diagnostic test that allows for the detection of MTB including its RIF resistance. Although the test is used for the diagnosis of tuberculosis (TB) in sputum samples worldwide, studies using fecal specimens are scarce. We therefore evaluated the efficacy of the Xpert MTB/RIF test for detection of MTB in fecal specimens obtained from adult pulmonary TB patients, confirmed by culture and/or molecular diagnostic methods.</jats:p> <jats:p>Methods. We conducted a retrospective case-control study to provide proof-of-concept regarding the efficacy of the Xpert MTB/RIF test using fecal samples for diagnosing pulmonary TB via detection of MTB in adult patients (≥20 years) at the Fukujuji Hospital in Tokyo, Japan.</jats:p> <jats:p>Results. Fecal specimens were obtained from 56 active pulmonary TB patients (including 48 sputum smear-positive and 8 sputum smear-negative patients), 10 non-TB patients (including 4 Myocobacterium avium complex infections), and 27 healthy individuals who were exposed to active pulmonary TB patients. The sensitivity of the fecal Xpert MTB/RIF was 100% (81.7%–100%) for detection of MTB in specimens from sputum smear-positive (1+ to 3+) patients, 81.0% (58.1%–94.6%) in specimens from sputum smear scanty positive patients, and 50.0% (15.7%–84.3%) in specimens from sputum smear-negative patients. Meanwhile, each of the fecal specimens from the non-TB group was negative for MTB (specificity 100%; 95% confidence interval, 86.2–100).</jats:p> <jats:p>Conclusions. The fecal Xpert MTB/RIF test could detect MTB in a large proportion of smear-positive pulmonary TB patients, without frequent false-positive results at a TB referral hospital in Japan.</jats:p> |
doi_str_mv | 10.1093/ofid/ofv074 |
facet_avail | Online, Free |
finc_class_facet | Medizin |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5My9vZmlkL29mdjA3NA |
imprint | Oxford University Press (OUP), 2015 |
imprint_str_mv | Oxford University Press (OUP), 2015 |
institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
issn | 2328-8957 |
issn_str_mv | 2328-8957 |
language | English |
last_indexed | 2024-03-01T17:14:49.954Z |
match_str | kokuto2015detectionofmycobacteriumtuberculosismtbinfecalspecimensfromadultsdiagnosedwithpulmonarytuberculosisusingthexpertmtbrifampicintest |
mega_collection | Oxford University Press (OUP) (CrossRef) |
physical | |
publishDate | 2015 |
publishDateSort | 2015 |
publisher | Oxford University Press (OUP) |
record_format | ai |
recordtype | ai |
series | Open Forum Infectious Diseases |
source_id | 49 |
spelling | Kokuto, Hiroyuki Sasaki, Yuka Yoshimatsu, Shoji Mizuno, Kazue Yi, Lina Mitarai, Satoshi 2328-8957 Oxford University Press (OUP) Infectious Diseases Oncology http://dx.doi.org/10.1093/ofid/ofv074 <jats:title>Abstract</jats:title> <jats:p>Background. The Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) is a fully automated diagnostic test that allows for the detection of MTB including its RIF resistance. Although the test is used for the diagnosis of tuberculosis (TB) in sputum samples worldwide, studies using fecal specimens are scarce. We therefore evaluated the efficacy of the Xpert MTB/RIF test for detection of MTB in fecal specimens obtained from adult pulmonary TB patients, confirmed by culture and/or molecular diagnostic methods.</jats:p> <jats:p>Methods. We conducted a retrospective case-control study to provide proof-of-concept regarding the efficacy of the Xpert MTB/RIF test using fecal samples for diagnosing pulmonary TB via detection of MTB in adult patients (≥20 years) at the Fukujuji Hospital in Tokyo, Japan.</jats:p> <jats:p>Results. Fecal specimens were obtained from 56 active pulmonary TB patients (including 48 sputum smear-positive and 8 sputum smear-negative patients), 10 non-TB patients (including 4 Myocobacterium avium complex infections), and 27 healthy individuals who were exposed to active pulmonary TB patients. The sensitivity of the fecal Xpert MTB/RIF was 100% (81.7%–100%) for detection of MTB in specimens from sputum smear-positive (1+ to 3+) patients, 81.0% (58.1%–94.6%) in specimens from sputum smear scanty positive patients, and 50.0% (15.7%–84.3%) in specimens from sputum smear-negative patients. Meanwhile, each of the fecal specimens from the non-TB group was negative for MTB (specificity 100%; 95% confidence interval, 86.2–100).</jats:p> <jats:p>Conclusions. The fecal Xpert MTB/RIF test could detect MTB in a large proportion of smear-positive pulmonary TB patients, without frequent false-positive results at a TB referral hospital in Japan.</jats:p> Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test Open Forum Infectious Diseases |
spellingShingle | Kokuto, Hiroyuki, Sasaki, Yuka, Yoshimatsu, Shoji, Mizuno, Kazue, Yi, Lina, Mitarai, Satoshi, Open Forum Infectious Diseases, Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test, Infectious Diseases, Oncology |
title | Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_full | Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_fullStr | Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_full_unstemmed | Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_short | Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
title_sort | detection of mycobacterium tuberculosis (mtb) in fecal specimens from adults diagnosed with pulmonary tuberculosis using the xpert mtb/rifampicin test |
title_unstemmed | Detection of Mycobacterium tuberculosis (MTB) in Fecal Specimens From Adults Diagnosed With Pulmonary Tuberculosis Using the Xpert MTB/Rifampicin Test |
topic | Infectious Diseases, Oncology |
url | http://dx.doi.org/10.1093/ofid/ofv074 |