Eintrag weiter verarbeiten
RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin
Gespeichert in:
| Zeitschriftentitel: | Molecular Biology of the Cell |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Molecular Biology of the Cell, 20, 2009, 1, S. 410-418 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
|
| Schlagwörter: |
| author_facet |
Klein, Ulf R. Haindl, Markus Nigg, Erich A. Muller, Stefan Klein, Ulf R. Haindl, Markus Nigg, Erich A. Muller, Stefan |
|---|---|
| author |
Klein, Ulf R. Haindl, Markus Nigg, Erich A. Muller, Stefan |
| spellingShingle |
Klein, Ulf R. Haindl, Markus Nigg, Erich A. Muller, Stefan Molecular Biology of the Cell RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin Cell Biology Molecular Biology |
| author_sort |
klein, ulf r. |
| spelling |
Klein, Ulf R. Haindl, Markus Nigg, Erich A. Muller, Stefan 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e08-05-0511 <jats:p>The ubiquitin-like SUMO system controls cellular key functions, and several lines of evidence point to a critical role of SUMO for mitotic progression. However, in mammalian cells mitotic substrates of sumoylation and the regulatory components involved are not well defined. Here, we identify Borealin, a component of the chromosomal passenger complex (CPC), as a mitotic target of SUMO. The CPC, which additionally comprises INCENP, Survivin, and Aurora B, regulates key mitotic events, including chromosome congression, the spindle assembly checkpoint, and cytokinesis. We show that Borealin is preferentially modified by SUMO2/3 and demonstrate that the modification is dynamically regulated during mitotic progression, peaking in early mitosis. Intriguingly, the SUMO ligase RanBP2 interacts with the CPC, stimulates SUMO modification of Borealin in vitro, and is required for its modification in vivo. Moreover, the SUMO isopeptidase SENP3 is a specific interaction partner of Borealin and catalyzes the removal of SUMO2/3 from Borealin. These data thus delineate a mitotic SUMO2/3 conjugation–deconjugation cycle of Borealin and further assign a regulatory function of RanBP2 and SENP3 in the mitotic SUMO pathway.</jats:p> RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin Molecular Biology of the Cell |
| doi_str_mv |
10.1091/mbc.e08-05-0511 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5MS9tYmMuZTA4LTA1LTA1MTE |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5MS9tYmMuZTA4LTA1LTA1MTE |
| institution |
DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Zi4 DE-Gla1 DE-15 DE-Pl11 DE-Rs1 DE-14 DE-105 |
| imprint |
American Society for Cell Biology (ASCB), 2009 |
| imprint_str_mv |
American Society for Cell Biology (ASCB), 2009 |
| issn |
1939-4586 1059-1524 |
| issn_str_mv |
1939-4586 1059-1524 |
| language |
English |
| mega_collection |
American Society for Cell Biology (ASCB) (CrossRef) |
| match_str |
klein2009ranbp2andsenp3functioninamitoticsumo23conjugationdeconjugationcycleonborealin |
| publishDateSort |
2009 |
| publisher |
American Society for Cell Biology (ASCB) |
| recordtype |
ai |
| record_format |
ai |
| series |
Molecular Biology of the Cell |
| source_id |
49 |
| title |
RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_unstemmed |
RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_full |
RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_fullStr |
RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_full_unstemmed |
RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_short |
RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_sort |
ranbp2 and senp3 function in a mitotic sumo2/3 conjugation-deconjugation cycle on borealin |
| topic |
Cell Biology Molecular Biology |
| url |
http://dx.doi.org/10.1091/mbc.e08-05-0511 |
| publishDate |
2009 |
| physical |
410-418 |
| description |
<jats:p>The ubiquitin-like SUMO system controls cellular key functions, and several lines of evidence point to a critical role of SUMO for mitotic progression. However, in mammalian cells mitotic substrates of sumoylation and the regulatory components involved are not well defined. Here, we identify Borealin, a component of the chromosomal passenger complex (CPC), as a mitotic target of SUMO. The CPC, which additionally comprises INCENP, Survivin, and Aurora B, regulates key mitotic events, including chromosome congression, the spindle assembly checkpoint, and cytokinesis. We show that Borealin is preferentially modified by SUMO2/3 and demonstrate that the modification is dynamically regulated during mitotic progression, peaking in early mitosis. Intriguingly, the SUMO ligase RanBP2 interacts with the CPC, stimulates SUMO modification of Borealin in vitro, and is required for its modification in vivo. Moreover, the SUMO isopeptidase SENP3 is a specific interaction partner of Borealin and catalyzes the removal of SUMO2/3 from Borealin. These data thus delineate a mitotic SUMO2/3 conjugation–deconjugation cycle of Borealin and further assign a regulatory function of RanBP2 and SENP3 in the mitotic SUMO pathway.</jats:p> |
| container_issue |
1 |
| container_start_page |
410 |
| container_title |
Molecular Biology of the Cell |
| container_volume |
20 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792341190075482113 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T16:15:57.805Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=RanBP2+and+SENP3+Function+in+a+Mitotic+SUMO2%2F3+Conjugation-Deconjugation+Cycle+on+Borealin&rft.date=2009-01-01&genre=article&issn=1939-4586&volume=20&issue=1&spage=410&epage=418&pages=410-418&jtitle=Molecular+Biology+of+the+Cell&atitle=RanBP2+and+SENP3+Function+in+a+Mitotic+SUMO2%2F3+Conjugation-Deconjugation+Cycle+on+Borealin&aulast=Muller&aufirst=Stefan&rft_id=info%3Adoi%2F10.1091%2Fmbc.e08-05-0511&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792341190075482113 |
| author | Klein, Ulf R., Haindl, Markus, Nigg, Erich A., Muller, Stefan |
| author_facet | Klein, Ulf R., Haindl, Markus, Nigg, Erich A., Muller, Stefan, Klein, Ulf R., Haindl, Markus, Nigg, Erich A., Muller, Stefan |
| author_sort | klein, ulf r. |
| container_issue | 1 |
| container_start_page | 410 |
| container_title | Molecular Biology of the Cell |
| container_volume | 20 |
| description | <jats:p>The ubiquitin-like SUMO system controls cellular key functions, and several lines of evidence point to a critical role of SUMO for mitotic progression. However, in mammalian cells mitotic substrates of sumoylation and the regulatory components involved are not well defined. Here, we identify Borealin, a component of the chromosomal passenger complex (CPC), as a mitotic target of SUMO. The CPC, which additionally comprises INCENP, Survivin, and Aurora B, regulates key mitotic events, including chromosome congression, the spindle assembly checkpoint, and cytokinesis. We show that Borealin is preferentially modified by SUMO2/3 and demonstrate that the modification is dynamically regulated during mitotic progression, peaking in early mitosis. Intriguingly, the SUMO ligase RanBP2 interacts with the CPC, stimulates SUMO modification of Borealin in vitro, and is required for its modification in vivo. Moreover, the SUMO isopeptidase SENP3 is a specific interaction partner of Borealin and catalyzes the removal of SUMO2/3 from Borealin. These data thus delineate a mitotic SUMO2/3 conjugation–deconjugation cycle of Borealin and further assign a regulatory function of RanBP2 and SENP3 in the mitotic SUMO pathway.</jats:p> |
| doi_str_mv | 10.1091/mbc.e08-05-0511 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA5MS9tYmMuZTA4LTA1LTA1MTE |
| imprint | American Society for Cell Biology (ASCB), 2009 |
| imprint_str_mv | American Society for Cell Biology (ASCB), 2009 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105 |
| issn | 1939-4586, 1059-1524 |
| issn_str_mv | 1939-4586, 1059-1524 |
| language | English |
| last_indexed | 2024-03-01T16:15:57.805Z |
| match_str | klein2009ranbp2andsenp3functioninamitoticsumo23conjugationdeconjugationcycleonborealin |
| mega_collection | American Society for Cell Biology (ASCB) (CrossRef) |
| physical | 410-418 |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | American Society for Cell Biology (ASCB) |
| record_format | ai |
| recordtype | ai |
| series | Molecular Biology of the Cell |
| source_id | 49 |
| spelling | Klein, Ulf R. Haindl, Markus Nigg, Erich A. Muller, Stefan 1059-1524 1939-4586 American Society for Cell Biology (ASCB) Cell Biology Molecular Biology http://dx.doi.org/10.1091/mbc.e08-05-0511 <jats:p>The ubiquitin-like SUMO system controls cellular key functions, and several lines of evidence point to a critical role of SUMO for mitotic progression. However, in mammalian cells mitotic substrates of sumoylation and the regulatory components involved are not well defined. Here, we identify Borealin, a component of the chromosomal passenger complex (CPC), as a mitotic target of SUMO. The CPC, which additionally comprises INCENP, Survivin, and Aurora B, regulates key mitotic events, including chromosome congression, the spindle assembly checkpoint, and cytokinesis. We show that Borealin is preferentially modified by SUMO2/3 and demonstrate that the modification is dynamically regulated during mitotic progression, peaking in early mitosis. Intriguingly, the SUMO ligase RanBP2 interacts with the CPC, stimulates SUMO modification of Borealin in vitro, and is required for its modification in vivo. Moreover, the SUMO isopeptidase SENP3 is a specific interaction partner of Borealin and catalyzes the removal of SUMO2/3 from Borealin. These data thus delineate a mitotic SUMO2/3 conjugation–deconjugation cycle of Borealin and further assign a regulatory function of RanBP2 and SENP3 in the mitotic SUMO pathway.</jats:p> RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin Molecular Biology of the Cell |
| spellingShingle | Klein, Ulf R., Haindl, Markus, Nigg, Erich A., Muller, Stefan, Molecular Biology of the Cell, RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin, Cell Biology, Molecular Biology |
| title | RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_full | RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_fullStr | RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_full_unstemmed | RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_short | RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| title_sort | ranbp2 and senp3 function in a mitotic sumo2/3 conjugation-deconjugation cycle on borealin |
| title_unstemmed | RanBP2 and SENP3 Function in a Mitotic SUMO2/3 Conjugation-Deconjugation Cycle on Borealin |
| topic | Cell Biology, Molecular Biology |
| url | http://dx.doi.org/10.1091/mbc.e08-05-0511 |