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Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit.
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| Zeitschriftentitel: | Cell Regulation |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Cell Regulation, 1, 1989, 1, S. 125-134 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
American Society for Cell Biology (ASCB)
|
| Schlagwörter: |
| author_facet |
Schmidt, C J Garen-Fazio, S Chow, Y K Neer, E J Schmidt, C J Garen-Fazio, S Chow, Y K Neer, E J |
|---|---|
| author |
Schmidt, C J Garen-Fazio, S Chow, Y K Neer, E J |
| spellingShingle |
Schmidt, C J Garen-Fazio, S Chow, Y K Neer, E J Cell Regulation Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. General Medicine |
| author_sort |
schmidt, c j |
| spelling |
Schmidt, C J Garen-Fazio, S Chow, Y K Neer, E J 1044-2030 American Society for Cell Biology (ASCB) General Medicine http://dx.doi.org/10.1091/mbc.1.1.125 <jats:p> Guanine nucleotide-binding proteins (G proteins) mediate signals between activated cell-surface receptors and cellular effectors. A bovine G-protein alpha-subunit cDNA has been used to isolate similar sequences from Drosophila genomic and cDNA libraries. One class, which we call DG alpha 0, hybridized to position 47A on the second chromosome of Drosophila. The nucleotide sequence of the protein coding region of one cDNA has been determined, revealing an alpha subunit that is 81% identical with rat alpha 0. The cDNA hybridizes strongly to a 3.8 kb mRNA and weakly with a 5.3 kb message. Antibodies raised against a trp-E-DG alpha 0 fusion protein recognized a 39,000 Da protein in Drosophila extracts. In situ hybridization to adult Drosophila sections combined with immunohistochemical studies revealed expression throughout the optic lobes and central brain and in the thoracic and abdominal ganglia. DG alpha 0 message and protein were also detected in the antennae, oocytes, and ovarian nurse cells. The neuronal expression of this gene is similar to mammalian alpha 0, which is most abundantly expressed in the brain. </jats:p> Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. Cell Regulation |
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American Society for Cell Biology (ASCB), 1989 |
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| title |
Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_unstemmed |
Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_full |
Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_fullStr |
Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_full_unstemmed |
Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_short |
Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_sort |
neuronal expression of a newly identified drosophila melanogaster g protein alpha 0 subunit. |
| topic |
General Medicine |
| url |
http://dx.doi.org/10.1091/mbc.1.1.125 |
| publishDate |
1989 |
| physical |
125-134 |
| description |
<jats:p> Guanine nucleotide-binding proteins (G proteins) mediate signals between activated cell-surface receptors and cellular effectors. A bovine G-protein alpha-subunit cDNA has been used to isolate similar sequences from Drosophila genomic and cDNA libraries. One class, which we call DG alpha 0, hybridized to position 47A on the second chromosome of Drosophila. The nucleotide sequence of the protein coding region of one cDNA has been determined, revealing an alpha subunit that is 81% identical with rat alpha 0. The cDNA hybridizes strongly to a 3.8 kb mRNA and weakly with a 5.3 kb message. Antibodies raised against a trp-E-DG alpha 0 fusion protein recognized a 39,000 Da protein in Drosophila extracts. In situ hybridization to adult Drosophila sections combined with immunohistochemical studies revealed expression throughout the optic lobes and central brain and in the thoracic and abdominal ganglia. DG alpha 0 message and protein were also detected in the antennae, oocytes, and ovarian nurse cells. The neuronal expression of this gene is similar to mammalian alpha 0, which is most abundantly expressed in the brain. </jats:p> |
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| author | Schmidt, C J, Garen-Fazio, S, Chow, Y K, Neer, E J |
| author_facet | Schmidt, C J, Garen-Fazio, S, Chow, Y K, Neer, E J, Schmidt, C J, Garen-Fazio, S, Chow, Y K, Neer, E J |
| author_sort | schmidt, c j |
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| description | <jats:p> Guanine nucleotide-binding proteins (G proteins) mediate signals between activated cell-surface receptors and cellular effectors. A bovine G-protein alpha-subunit cDNA has been used to isolate similar sequences from Drosophila genomic and cDNA libraries. One class, which we call DG alpha 0, hybridized to position 47A on the second chromosome of Drosophila. The nucleotide sequence of the protein coding region of one cDNA has been determined, revealing an alpha subunit that is 81% identical with rat alpha 0. The cDNA hybridizes strongly to a 3.8 kb mRNA and weakly with a 5.3 kb message. Antibodies raised against a trp-E-DG alpha 0 fusion protein recognized a 39,000 Da protein in Drosophila extracts. In situ hybridization to adult Drosophila sections combined with immunohistochemical studies revealed expression throughout the optic lobes and central brain and in the thoracic and abdominal ganglia. DG alpha 0 message and protein were also detected in the antennae, oocytes, and ovarian nurse cells. The neuronal expression of this gene is similar to mammalian alpha 0, which is most abundantly expressed in the brain. </jats:p> |
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| imprint | American Society for Cell Biology (ASCB), 1989 |
| imprint_str_mv | American Society for Cell Biology (ASCB), 1989 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
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| physical | 125-134 |
| publishDate | 1989 |
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| series | Cell Regulation |
| source_id | 49 |
| spelling | Schmidt, C J Garen-Fazio, S Chow, Y K Neer, E J 1044-2030 American Society for Cell Biology (ASCB) General Medicine http://dx.doi.org/10.1091/mbc.1.1.125 <jats:p> Guanine nucleotide-binding proteins (G proteins) mediate signals between activated cell-surface receptors and cellular effectors. A bovine G-protein alpha-subunit cDNA has been used to isolate similar sequences from Drosophila genomic and cDNA libraries. One class, which we call DG alpha 0, hybridized to position 47A on the second chromosome of Drosophila. The nucleotide sequence of the protein coding region of one cDNA has been determined, revealing an alpha subunit that is 81% identical with rat alpha 0. The cDNA hybridizes strongly to a 3.8 kb mRNA and weakly with a 5.3 kb message. Antibodies raised against a trp-E-DG alpha 0 fusion protein recognized a 39,000 Da protein in Drosophila extracts. In situ hybridization to adult Drosophila sections combined with immunohistochemical studies revealed expression throughout the optic lobes and central brain and in the thoracic and abdominal ganglia. DG alpha 0 message and protein were also detected in the antennae, oocytes, and ovarian nurse cells. The neuronal expression of this gene is similar to mammalian alpha 0, which is most abundantly expressed in the brain. </jats:p> Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. Cell Regulation |
| spellingShingle | Schmidt, C J, Garen-Fazio, S, Chow, Y K, Neer, E J, Cell Regulation, Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit., General Medicine |
| title | Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_full | Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_fullStr | Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_full_unstemmed | Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_short | Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| title_sort | neuronal expression of a newly identified drosophila melanogaster g protein alpha 0 subunit. |
| title_unstemmed | Neuronal expression of a newly identified Drosophila melanogaster G protein alpha 0 subunit. |
| topic | General Medicine |
| url | http://dx.doi.org/10.1091/mbc.1.1.125 |