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F-actin aggregates in transformed cells.

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Zeitschriftentitel: The Journal of cell biology
Personen und Körperschaften: Carley, W W, Barak, L S, Webb, W W
In: The Journal of cell biology, 90, 1981, 3, S. 797-802
Format: E-Article
Sprache: Englisch
veröffentlicht:
Rockefeller University Press
Schlagwörter:
Cell Biology
author_facet Carley, W W
Barak, L S
Webb, W W
Carley, W W
Barak, L S
Webb, W W
author Carley, W W
Barak, L S
Webb, W W
spellingShingle Carley, W W
Barak, L S
Webb, W W
The Journal of cell biology
F-actin aggregates in transformed cells.
Cell Biology
author_sort carley, w w
spelling Carley, W W Barak, L S Webb, W W 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.90.3.797 <jats:p>Polymerized actin has been found aggregated into distinctive patches inside transformed cells in culture. The F-actin-specific fluorescent probe, nitrobenzoxadiazole-phallacidin, labels these F-actin aggregates near the ventral cell surface of cells transformed by RNA or DNA tumor viruses, or by chemical mutagens, or spontaneously. Their appearance in all eight transformed cell types studied suggests their ubiquity and involvement in transformation morphology. Actin patches developed in normal rat kidney (NRK) cells transformed by a temperature-sensitive mutant of Rous sarcoma virus (LA23-NRK) within 30 min after a shift from the nonpermissive (39 degrees C) to the permissive temperature (32 degrees C). Patch appearance paralleling viral src gene expression tends to implicate pp60src kinase activity in destabilizing the cytoskeleton. However, appearance of the actin aggregates in cells not transformed by retrovirus calls for alternative mechanisms, perhaps involving an endogenous kinase, for this apparently common trait.</jats:p> F-actin aggregates in transformed cells. The Journal of cell biology
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series The Journal of cell biology
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title F-actin aggregates in transformed cells.
title_unstemmed F-actin aggregates in transformed cells.
title_full F-actin aggregates in transformed cells.
title_fullStr F-actin aggregates in transformed cells.
title_full_unstemmed F-actin aggregates in transformed cells.
title_short F-actin aggregates in transformed cells.
title_sort f-actin aggregates in transformed cells.
topic Cell Biology
url http://dx.doi.org/10.1083/jcb.90.3.797
publishDate 1981
physical 797-802
description <jats:p>Polymerized actin has been found aggregated into distinctive patches inside transformed cells in culture. The F-actin-specific fluorescent probe, nitrobenzoxadiazole-phallacidin, labels these F-actin aggregates near the ventral cell surface of cells transformed by RNA or DNA tumor viruses, or by chemical mutagens, or spontaneously. Their appearance in all eight transformed cell types studied suggests their ubiquity and involvement in transformation morphology. Actin patches developed in normal rat kidney (NRK) cells transformed by a temperature-sensitive mutant of Rous sarcoma virus (LA23-NRK) within 30 min after a shift from the nonpermissive (39 degrees C) to the permissive temperature (32 degrees C). Patch appearance paralleling viral src gene expression tends to implicate pp60src kinase activity in destabilizing the cytoskeleton. However, appearance of the actin aggregates in cells not transformed by retrovirus calls for alternative mechanisms, perhaps involving an endogenous kinase, for this apparently common trait.</jats:p>
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author Carley, W W, Barak, L S, Webb, W W
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description <jats:p>Polymerized actin has been found aggregated into distinctive patches inside transformed cells in culture. The F-actin-specific fluorescent probe, nitrobenzoxadiazole-phallacidin, labels these F-actin aggregates near the ventral cell surface of cells transformed by RNA or DNA tumor viruses, or by chemical mutagens, or spontaneously. Their appearance in all eight transformed cell types studied suggests their ubiquity and involvement in transformation morphology. Actin patches developed in normal rat kidney (NRK) cells transformed by a temperature-sensitive mutant of Rous sarcoma virus (LA23-NRK) within 30 min after a shift from the nonpermissive (39 degrees C) to the permissive temperature (32 degrees C). Patch appearance paralleling viral src gene expression tends to implicate pp60src kinase activity in destabilizing the cytoskeleton. However, appearance of the actin aggregates in cells not transformed by retrovirus calls for alternative mechanisms, perhaps involving an endogenous kinase, for this apparently common trait.</jats:p>
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imprint Rockefeller University Press, 1981
imprint_str_mv Rockefeller University Press, 1981
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spelling Carley, W W Barak, L S Webb, W W 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.90.3.797 <jats:p>Polymerized actin has been found aggregated into distinctive patches inside transformed cells in culture. The F-actin-specific fluorescent probe, nitrobenzoxadiazole-phallacidin, labels these F-actin aggregates near the ventral cell surface of cells transformed by RNA or DNA tumor viruses, or by chemical mutagens, or spontaneously. Their appearance in all eight transformed cell types studied suggests their ubiquity and involvement in transformation morphology. Actin patches developed in normal rat kidney (NRK) cells transformed by a temperature-sensitive mutant of Rous sarcoma virus (LA23-NRK) within 30 min after a shift from the nonpermissive (39 degrees C) to the permissive temperature (32 degrees C). Patch appearance paralleling viral src gene expression tends to implicate pp60src kinase activity in destabilizing the cytoskeleton. However, appearance of the actin aggregates in cells not transformed by retrovirus calls for alternative mechanisms, perhaps involving an endogenous kinase, for this apparently common trait.</jats:p> F-actin aggregates in transformed cells. The Journal of cell biology
spellingShingle Carley, W W, Barak, L S, Webb, W W, The Journal of cell biology, F-actin aggregates in transformed cells., Cell Biology
title F-actin aggregates in transformed cells.
title_full F-actin aggregates in transformed cells.
title_fullStr F-actin aggregates in transformed cells.
title_full_unstemmed F-actin aggregates in transformed cells.
title_short F-actin aggregates in transformed cells.
title_sort f-actin aggregates in transformed cells.
title_unstemmed F-actin aggregates in transformed cells.
topic Cell Biology
url http://dx.doi.org/10.1083/jcb.90.3.797