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Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin.
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Zeitschriftentitel: | The Journal of cell biology |
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Personen und Körperschaften: | , , |
In: | The Journal of cell biology, 85, 1980, 2, S. 273-282 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Rockefeller University Press
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Schlagwörter: |
author_facet |
Nagata, K Sagara, J Ichikawa, Y Nagata, K Sagara, J Ichikawa, Y |
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author |
Nagata, K Sagara, J Ichikawa, Y |
spellingShingle |
Nagata, K Sagara, J Ichikawa, Y The Journal of cell biology Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. Cell Biology |
author_sort |
nagata, k |
spelling |
Nagata, K Sagara, J Ichikawa, Y 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.85.2.273 <jats:p>Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d of incubation with CM, when the Ml cells differentiated to macrophages and lost their mitotic activity, the actin content, F-actin ratio in total actin, and the actin synthesis showed an increase. A greater difference before and after differentiation was found in the ability of G-actin to polymerize. Actin harvested from CM-treated cells showed a greater ability to polymerize, depending on the increased concentration of MgCl2 and/or KCl and proteins, as compared with the actin from untreated Ml cells. Actin harvested from the Mml cell line, a macrophage line, had a particularly high polymerizability with or without CM treatment. In contrast, the actin from the D- subline, which is insensitive to CM, showed almost no polymerization.</jats:p> Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. The Journal of cell biology |
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10.1083/jcb.85.2.273 |
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Rockefeller University Press, 1980 |
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Rockefeller University Press, 1980 |
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1980 |
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Rockefeller University Press |
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The Journal of cell biology |
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49 |
title |
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_unstemmed |
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_full |
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_fullStr |
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_full_unstemmed |
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_short |
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_sort |
changes in contractile proteins during differentiation of myeloid leukemia cells. i. polymerization of actin. |
topic |
Cell Biology |
url |
http://dx.doi.org/10.1083/jcb.85.2.273 |
publishDate |
1980 |
physical |
273-282 |
description |
<jats:p>Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d of incubation with CM, when the Ml cells differentiated to macrophages and lost their mitotic activity, the actin content, F-actin ratio in total actin, and the actin synthesis showed an increase. A greater difference before and after differentiation was found in the ability of G-actin to polymerize. Actin harvested from CM-treated cells showed a greater ability to polymerize, depending on the increased concentration of MgCl2 and/or KCl and proteins, as compared with the actin from untreated Ml cells. Actin harvested from the Mml cell line, a macrophage line, had a particularly high polymerizability with or without CM treatment. In contrast, the actin from the D- subline, which is insensitive to CM, showed almost no polymerization.</jats:p> |
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author | Nagata, K, Sagara, J, Ichikawa, Y |
author_facet | Nagata, K, Sagara, J, Ichikawa, Y, Nagata, K, Sagara, J, Ichikawa, Y |
author_sort | nagata, k |
container_issue | 2 |
container_start_page | 273 |
container_title | The Journal of cell biology |
container_volume | 85 |
description | <jats:p>Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d of incubation with CM, when the Ml cells differentiated to macrophages and lost their mitotic activity, the actin content, F-actin ratio in total actin, and the actin synthesis showed an increase. A greater difference before and after differentiation was found in the ability of G-actin to polymerize. Actin harvested from CM-treated cells showed a greater ability to polymerize, depending on the increased concentration of MgCl2 and/or KCl and proteins, as compared with the actin from untreated Ml cells. Actin harvested from the Mml cell line, a macrophage line, had a particularly high polymerizability with or without CM treatment. In contrast, the actin from the D- subline, which is insensitive to CM, showed almost no polymerization.</jats:p> |
doi_str_mv | 10.1083/jcb.85.2.273 |
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imprint | Rockefeller University Press, 1980 |
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institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
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publishDate | 1980 |
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publisher | Rockefeller University Press |
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recordtype | ai |
series | The Journal of cell biology |
source_id | 49 |
spelling | Nagata, K Sagara, J Ichikawa, Y 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.85.2.273 <jats:p>Quantitative and qualitative changes in cellular actin were followed during differentiation of a myeloid leukemia cell line, namely Ml, which was inducible with conditioned medium (CM). During 3 d of incubation with CM, when the Ml cells differentiated to macrophages and lost their mitotic activity, the actin content, F-actin ratio in total actin, and the actin synthesis showed an increase. A greater difference before and after differentiation was found in the ability of G-actin to polymerize. Actin harvested from CM-treated cells showed a greater ability to polymerize, depending on the increased concentration of MgCl2 and/or KCl and proteins, as compared with the actin from untreated Ml cells. Actin harvested from the Mml cell line, a macrophage line, had a particularly high polymerizability with or without CM treatment. In contrast, the actin from the D- subline, which is insensitive to CM, showed almost no polymerization.</jats:p> Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. The Journal of cell biology |
spellingShingle | Nagata, K, Sagara, J, Ichikawa, Y, The Journal of cell biology, Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin., Cell Biology |
title | Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_full | Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_fullStr | Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_full_unstemmed | Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_short | Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
title_sort | changes in contractile proteins during differentiation of myeloid leukemia cells. i. polymerization of actin. |
title_unstemmed | Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin. |
topic | Cell Biology |
url | http://dx.doi.org/10.1083/jcb.85.2.273 |