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ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage
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| Zeitschriftentitel: | Journal of Cell Biology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Journal of Cell Biology, 192, 2011, 2, S. 219-227 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Göhler, Thomas Sabbioneda, Simone Green, Catherine M. Lehmann, Alan R. Göhler, Thomas Sabbioneda, Simone Green, Catherine M. Lehmann, Alan R. |
|---|---|
| author |
Göhler, Thomas Sabbioneda, Simone Green, Catherine M. Lehmann, Alan R. |
| spellingShingle |
Göhler, Thomas Sabbioneda, Simone Green, Catherine M. Lehmann, Alan R. Journal of Cell Biology ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage Cell Biology |
| author_sort |
göhler, thomas |
| spelling |
Göhler, Thomas Sabbioneda, Simone Green, Catherine M. Lehmann, Alan R. 1540-8140 0021-9525 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.201008076 <jats:p>DNA polymerase η (polη) belongs to the Y-family of DNA polymerases and facilitates translesion synthesis past UV damage. We show that, after UV irradiation, polη becomes phosphorylated at Ser601 by the ataxia-telangiectasia mutated and Rad3-related (ATR) kinase. DNA damage–induced phosphorylation of polη depends on its physical interaction with Rad18 but is independent of PCNA monoubiquitination. It requires the ubiquitin-binding domain of polη but not its PCNA-interacting motif. ATR-dependent phosphorylation of polη is necessary to restore normal survival and postreplication repair after ultraviolet irradiation in xeroderma pigmentosum variant fibroblasts, and is involved in the checkpoint response to UV damage. Taken together, our results provide evidence for a link between DNA damage–induced checkpoint activation and translesion synthesis in mammalian cells.</jats:p> ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage Journal of Cell Biology |
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| title |
ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_unstemmed |
ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_full |
ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_fullStr |
ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_full_unstemmed |
ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_short |
ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_sort |
atr-mediated phosphorylation of dna polymerase η is needed for efficient recovery from uv damage |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1083/jcb.201008076 |
| publishDate |
2011 |
| physical |
219-227 |
| description |
<jats:p>DNA polymerase η (polη) belongs to the Y-family of DNA polymerases and facilitates translesion synthesis past UV damage. We show that, after UV irradiation, polη becomes phosphorylated at Ser601 by the ataxia-telangiectasia mutated and Rad3-related (ATR) kinase. DNA damage–induced phosphorylation of polη depends on its physical interaction with Rad18 but is independent of PCNA monoubiquitination. It requires the ubiquitin-binding domain of polη but not its PCNA-interacting motif. ATR-dependent phosphorylation of polη is necessary to restore normal survival and postreplication repair after ultraviolet irradiation in xeroderma pigmentosum variant fibroblasts, and is involved in the checkpoint response to UV damage. Taken together, our results provide evidence for a link between DNA damage–induced checkpoint activation and translesion synthesis in mammalian cells.</jats:p> |
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| author | Göhler, Thomas, Sabbioneda, Simone, Green, Catherine M., Lehmann, Alan R. |
| author_facet | Göhler, Thomas, Sabbioneda, Simone, Green, Catherine M., Lehmann, Alan R., Göhler, Thomas, Sabbioneda, Simone, Green, Catherine M., Lehmann, Alan R. |
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| description | <jats:p>DNA polymerase η (polη) belongs to the Y-family of DNA polymerases and facilitates translesion synthesis past UV damage. We show that, after UV irradiation, polη becomes phosphorylated at Ser601 by the ataxia-telangiectasia mutated and Rad3-related (ATR) kinase. DNA damage–induced phosphorylation of polη depends on its physical interaction with Rad18 but is independent of PCNA monoubiquitination. It requires the ubiquitin-binding domain of polη but not its PCNA-interacting motif. ATR-dependent phosphorylation of polη is necessary to restore normal survival and postreplication repair after ultraviolet irradiation in xeroderma pigmentosum variant fibroblasts, and is involved in the checkpoint response to UV damage. Taken together, our results provide evidence for a link between DNA damage–induced checkpoint activation and translesion synthesis in mammalian cells.</jats:p> |
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| physical | 219-227 |
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| series | Journal of Cell Biology |
| source_id | 49 |
| spelling | Göhler, Thomas Sabbioneda, Simone Green, Catherine M. Lehmann, Alan R. 1540-8140 0021-9525 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.201008076 <jats:p>DNA polymerase η (polη) belongs to the Y-family of DNA polymerases and facilitates translesion synthesis past UV damage. We show that, after UV irradiation, polη becomes phosphorylated at Ser601 by the ataxia-telangiectasia mutated and Rad3-related (ATR) kinase. DNA damage–induced phosphorylation of polη depends on its physical interaction with Rad18 but is independent of PCNA monoubiquitination. It requires the ubiquitin-binding domain of polη but not its PCNA-interacting motif. ATR-dependent phosphorylation of polη is necessary to restore normal survival and postreplication repair after ultraviolet irradiation in xeroderma pigmentosum variant fibroblasts, and is involved in the checkpoint response to UV damage. Taken together, our results provide evidence for a link between DNA damage–induced checkpoint activation and translesion synthesis in mammalian cells.</jats:p> ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage Journal of Cell Biology |
| spellingShingle | Göhler, Thomas, Sabbioneda, Simone, Green, Catherine M., Lehmann, Alan R., Journal of Cell Biology, ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage, Cell Biology |
| title | ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_full | ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_fullStr | ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_full_unstemmed | ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_short | ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| title_sort | atr-mediated phosphorylation of dna polymerase η is needed for efficient recovery from uv damage |
| title_unstemmed | ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1083/jcb.201008076 |