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Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells
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| Zeitschriftentitel: | The Journal of Cell Biology |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | The Journal of Cell Biology, 153, 2001, 6, S. 1277-1286 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Dimitroff, Charles J. Lee, Jack Y. Rafii, Shahin Fuhlbrigge, Robert C. Sackstein, Robert Dimitroff, Charles J. Lee, Jack Y. Rafii, Shahin Fuhlbrigge, Robert C. Sackstein, Robert |
|---|---|
| author |
Dimitroff, Charles J. Lee, Jack Y. Rafii, Shahin Fuhlbrigge, Robert C. Sackstein, Robert |
| spellingShingle |
Dimitroff, Charles J. Lee, Jack Y. Rafii, Shahin Fuhlbrigge, Robert C. Sackstein, Robert The Journal of Cell Biology Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells Cell Biology |
| author_sort |
dimitroff, charles j. |
| spelling |
Dimitroff, Charles J. Lee, Jack Y. Rafii, Shahin Fuhlbrigge, Robert C. Sackstein, Robert 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.153.6.1277 <jats:p>E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). Though it is known that a glycoform of PSGL-1 (CLA) functions as the principal E-selectin ligand on human T lymphocytes, the E-selectin ligand(s) of human HPCs has not been identified. We used a shear-based adherence assay to analyze and define the E-selectin ligand activity of membrane proteins from human HPCs. Our data show that PSGL-1 expressed on human HPCs is an E-selectin ligand, and that HPCs also express a previously unrecognized E-selectin ligand, CD44. The E-selectin ligand activity of CD44 is conferred by the elaboration of sialylated, fucosylated binding determinants on N-glycans. This glycoform of CD44 is expressed on primitive CD34+ human HPCs, but not on more mature hematopoietic cells. Under physiologic flow conditions, this molecule mediates E-selectin–dependent rolling interactions over a wider shear range than that of PSGL-1, and promotes human HPC rolling interactions on E-selectin expressed on human BM endothelial cells. These findings offer new insights into the structural biology and physiology of CD44, and into the molecular basis of E-selectin–dependent adhesive interactions that direct homing of human HPC to BM.</jats:p> Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells The Journal of Cell Biology |
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| title |
Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_unstemmed |
Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_full |
Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_fullStr |
Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_full_unstemmed |
Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_short |
Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_sort |
cd44 is a major e-selectin ligand on human hematopoietic progenitor cells |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1083/jcb.153.6.1277 |
| publishDate |
2001 |
| physical |
1277-1286 |
| description |
<jats:p>E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). Though it is known that a glycoform of PSGL-1 (CLA) functions as the principal E-selectin ligand on human T lymphocytes, the E-selectin ligand(s) of human HPCs has not been identified. We used a shear-based adherence assay to analyze and define the E-selectin ligand activity of membrane proteins from human HPCs. Our data show that PSGL-1 expressed on human HPCs is an E-selectin ligand, and that HPCs also express a previously unrecognized E-selectin ligand, CD44. The E-selectin ligand activity of CD44 is conferred by the elaboration of sialylated, fucosylated binding determinants on N-glycans. This glycoform of CD44 is expressed on primitive CD34+ human HPCs, but not on more mature hematopoietic cells. Under physiologic flow conditions, this molecule mediates E-selectin–dependent rolling interactions over a wider shear range than that of PSGL-1, and promotes human HPC rolling interactions on E-selectin expressed on human BM endothelial cells. These findings offer new insights into the structural biology and physiology of CD44, and into the molecular basis of E-selectin–dependent adhesive interactions that direct homing of human HPC to BM.</jats:p> |
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| author | Dimitroff, Charles J., Lee, Jack Y., Rafii, Shahin, Fuhlbrigge, Robert C., Sackstein, Robert |
| author_facet | Dimitroff, Charles J., Lee, Jack Y., Rafii, Shahin, Fuhlbrigge, Robert C., Sackstein, Robert, Dimitroff, Charles J., Lee, Jack Y., Rafii, Shahin, Fuhlbrigge, Robert C., Sackstein, Robert |
| author_sort | dimitroff, charles j. |
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| description | <jats:p>E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). Though it is known that a glycoform of PSGL-1 (CLA) functions as the principal E-selectin ligand on human T lymphocytes, the E-selectin ligand(s) of human HPCs has not been identified. We used a shear-based adherence assay to analyze and define the E-selectin ligand activity of membrane proteins from human HPCs. Our data show that PSGL-1 expressed on human HPCs is an E-selectin ligand, and that HPCs also express a previously unrecognized E-selectin ligand, CD44. The E-selectin ligand activity of CD44 is conferred by the elaboration of sialylated, fucosylated binding determinants on N-glycans. This glycoform of CD44 is expressed on primitive CD34+ human HPCs, but not on more mature hematopoietic cells. Under physiologic flow conditions, this molecule mediates E-selectin–dependent rolling interactions over a wider shear range than that of PSGL-1, and promotes human HPC rolling interactions on E-selectin expressed on human BM endothelial cells. These findings offer new insights into the structural biology and physiology of CD44, and into the molecular basis of E-selectin–dependent adhesive interactions that direct homing of human HPC to BM.</jats:p> |
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| spelling | Dimitroff, Charles J. Lee, Jack Y. Rafii, Shahin Fuhlbrigge, Robert C. Sackstein, Robert 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.153.6.1277 <jats:p>E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). Though it is known that a glycoform of PSGL-1 (CLA) functions as the principal E-selectin ligand on human T lymphocytes, the E-selectin ligand(s) of human HPCs has not been identified. We used a shear-based adherence assay to analyze and define the E-selectin ligand activity of membrane proteins from human HPCs. Our data show that PSGL-1 expressed on human HPCs is an E-selectin ligand, and that HPCs also express a previously unrecognized E-selectin ligand, CD44. The E-selectin ligand activity of CD44 is conferred by the elaboration of sialylated, fucosylated binding determinants on N-glycans. This glycoform of CD44 is expressed on primitive CD34+ human HPCs, but not on more mature hematopoietic cells. Under physiologic flow conditions, this molecule mediates E-selectin–dependent rolling interactions over a wider shear range than that of PSGL-1, and promotes human HPC rolling interactions on E-selectin expressed on human BM endothelial cells. These findings offer new insights into the structural biology and physiology of CD44, and into the molecular basis of E-selectin–dependent adhesive interactions that direct homing of human HPC to BM.</jats:p> Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells The Journal of Cell Biology |
| spellingShingle | Dimitroff, Charles J., Lee, Jack Y., Rafii, Shahin, Fuhlbrigge, Robert C., Sackstein, Robert, The Journal of Cell Biology, Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells, Cell Biology |
| title | Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_full | Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_fullStr | Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_full_unstemmed | Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_short | Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| title_sort | cd44 is a major e-selectin ligand on human hematopoietic progenitor cells |
| title_unstemmed | Cd44 Is a Major E-Selectin Ligand on Human Hematopoietic Progenitor Cells |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1083/jcb.153.6.1277 |