Eintrag weiter verarbeiten
Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells.
Gespeichert in:
Zeitschriftentitel: | The Journal of cell biology |
---|---|
Personen und Körperschaften: | , , , , |
In: | The Journal of cell biology, 123, 1993, 6, S. 1835-1843 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Rockefeller University Press
|
Schlagwörter: |
author_facet |
Sharma, N D'Arcangelo, G Kleinlaus, A Halegoua, S Trimmer, J S Sharma, N D'Arcangelo, G Kleinlaus, A Halegoua, S Trimmer, J S |
---|---|
author |
Sharma, N D'Arcangelo, G Kleinlaus, A Halegoua, S Trimmer, J S |
spellingShingle |
Sharma, N D'Arcangelo, G Kleinlaus, A Halegoua, S Trimmer, J S The Journal of cell biology Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. Cell Biology |
author_sort |
sharma, n |
spelling |
Sharma, N D'Arcangelo, G Kleinlaus, A Halegoua, S Trimmer, J S 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.123.6.1835 <jats:p>We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifler K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of M(r) = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 k+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.</jats:p> Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. The Journal of cell biology |
doi_str_mv |
10.1083/jcb.123.6.1835 |
facet_avail |
Online Free |
finc_class_facet |
Biologie |
format |
ElectronicArticle |
fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTIzLjYuMTgzNQ |
id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTIzLjYuMTgzNQ |
institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
imprint |
Rockefeller University Press, 1993 |
imprint_str_mv |
Rockefeller University Press, 1993 |
issn |
1540-8140 0021-9525 |
issn_str_mv |
1540-8140 0021-9525 |
language |
English |
mega_collection |
Rockefeller University Press (CrossRef) |
match_str |
sharma1993nervegrowthfactorregulatestheabundanceanddistributionofkchannelsinpc12cells |
publishDateSort |
1993 |
publisher |
Rockefeller University Press |
recordtype |
ai |
record_format |
ai |
series |
The Journal of cell biology |
source_id |
49 |
title |
Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_unstemmed |
Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_full |
Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_fullStr |
Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_full_unstemmed |
Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_short |
Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_sort |
nerve growth factor regulates the abundance and distribution of k+ channels in pc12 cells. |
topic |
Cell Biology |
url |
http://dx.doi.org/10.1083/jcb.123.6.1835 |
publishDate |
1993 |
physical |
1835-1843 |
description |
<jats:p>We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifler K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of M(r) = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 k+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.</jats:p> |
container_issue |
6 |
container_start_page |
1835 |
container_title |
The Journal of cell biology |
container_volume |
123 |
format_de105 |
Article, E-Article |
format_de14 |
Article, E-Article |
format_de15 |
Article, E-Article |
format_de520 |
Article, E-Article |
format_de540 |
Article, E-Article |
format_dech1 |
Article, E-Article |
format_ded117 |
Article, E-Article |
format_degla1 |
E-Article |
format_del152 |
Buch |
format_del189 |
Article, E-Article |
format_dezi4 |
Article |
format_dezwi2 |
Article, E-Article |
format_finc |
Article, E-Article |
format_nrw |
Article, E-Article |
_version_ |
1792342847330975750 |
geogr_code |
not assigned |
last_indexed |
2024-03-01T16:42:18.804Z |
geogr_code_person |
not assigned |
openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Nerve+growth+factor+regulates+the+abundance+and+distribution+of+K%2B+channels+in+PC12+cells.&rft.date=1993-12-15&genre=article&issn=1540-8140&volume=123&issue=6&spage=1835&epage=1843&pages=1835-1843&jtitle=The+Journal+of+cell+biology&atitle=Nerve+growth+factor+regulates+the+abundance+and+distribution+of+K%2B+channels+in+PC12+cells.&aulast=Trimmer&aufirst=J+S&rft_id=info%3Adoi%2F10.1083%2Fjcb.123.6.1835&rft.language%5B0%5D=eng |
SOLR | |
_version_ | 1792342847330975750 |
author | Sharma, N, D'Arcangelo, G, Kleinlaus, A, Halegoua, S, Trimmer, J S |
author_facet | Sharma, N, D'Arcangelo, G, Kleinlaus, A, Halegoua, S, Trimmer, J S, Sharma, N, D'Arcangelo, G, Kleinlaus, A, Halegoua, S, Trimmer, J S |
author_sort | sharma, n |
container_issue | 6 |
container_start_page | 1835 |
container_title | The Journal of cell biology |
container_volume | 123 |
description | <jats:p>We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifler K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of M(r) = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 k+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.</jats:p> |
doi_str_mv | 10.1083/jcb.123.6.1835 |
facet_avail | Online, Free |
finc_class_facet | Biologie |
format | ElectronicArticle |
format_de105 | Article, E-Article |
format_de14 | Article, E-Article |
format_de15 | Article, E-Article |
format_de520 | Article, E-Article |
format_de540 | Article, E-Article |
format_dech1 | Article, E-Article |
format_ded117 | Article, E-Article |
format_degla1 | E-Article |
format_del152 | Buch |
format_del189 | Article, E-Article |
format_dezi4 | Article |
format_dezwi2 | Article, E-Article |
format_finc | Article, E-Article |
format_nrw | Article, E-Article |
geogr_code | not assigned |
geogr_code_person | not assigned |
id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTIzLjYuMTgzNQ |
imprint | Rockefeller University Press, 1993 |
imprint_str_mv | Rockefeller University Press, 1993 |
institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
issn | 1540-8140, 0021-9525 |
issn_str_mv | 1540-8140, 0021-9525 |
language | English |
last_indexed | 2024-03-01T16:42:18.804Z |
match_str | sharma1993nervegrowthfactorregulatestheabundanceanddistributionofkchannelsinpc12cells |
mega_collection | Rockefeller University Press (CrossRef) |
physical | 1835-1843 |
publishDate | 1993 |
publishDateSort | 1993 |
publisher | Rockefeller University Press |
record_format | ai |
recordtype | ai |
series | The Journal of cell biology |
source_id | 49 |
spelling | Sharma, N D'Arcangelo, G Kleinlaus, A Halegoua, S Trimmer, J S 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.123.6.1835 <jats:p>We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifler K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of M(r) = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 k+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.</jats:p> Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. The Journal of cell biology |
spellingShingle | Sharma, N, D'Arcangelo, G, Kleinlaus, A, Halegoua, S, Trimmer, J S, The Journal of cell biology, Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells., Cell Biology |
title | Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_full | Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_fullStr | Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_full_unstemmed | Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_short | Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
title_sort | nerve growth factor regulates the abundance and distribution of k+ channels in pc12 cells. |
title_unstemmed | Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. |
topic | Cell Biology |
url | http://dx.doi.org/10.1083/jcb.123.6.1835 |