Eintrag weiter verarbeiten
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
Gespeichert in:
| Zeitschriftentitel: | The Journal of cell biology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | The Journal of cell biology, 119, 1992, 3, S. 493-501 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Gavrieli, Y Sherman, Y Ben-Sasson, S A Gavrieli, Y Sherman, Y Ben-Sasson, S A |
|---|---|
| author |
Gavrieli, Y Sherman, Y Ben-Sasson, S A |
| spellingShingle |
Gavrieli, Y Sherman, Y Ben-Sasson, S A The Journal of cell biology Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. Cell Biology |
| author_sort |
gavrieli, y |
| spelling |
Gavrieli, Y Sherman, Y Ben-Sasson, S A 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.119.3.493 <jats:p>Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxy-transferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.</jats:p> Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. The Journal of cell biology |
| doi_str_mv |
10.1083/jcb.119.3.493 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTE5LjMuNDkz |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTE5LjMuNDkz |
| institution |
DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Rs1 DE-Pl11 DE-105 DE-14 |
| imprint |
Rockefeller University Press, 1992 |
| imprint_str_mv |
Rockefeller University Press, 1992 |
| issn |
0021-9525 1540-8140 |
| issn_str_mv |
0021-9525 1540-8140 |
| language |
English |
| mega_collection |
Rockefeller University Press (CrossRef) |
| match_str |
gavrieli1992identificationofprogrammedcelldeathinsituviaspecificlabelingofnucleardnafragmentation |
| publishDateSort |
1992 |
| publisher |
Rockefeller University Press |
| recordtype |
ai |
| record_format |
ai |
| series |
The Journal of cell biology |
| source_id |
49 |
| title |
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_unstemmed |
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_full |
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_fullStr |
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_full_unstemmed |
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_short |
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_sort |
identification of programmed cell death in situ via specific labeling of nuclear dna fragmentation. |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1083/jcb.119.3.493 |
| publishDate |
1992 |
| physical |
493-501 |
| description |
<jats:p>Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxy-transferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.</jats:p> |
| container_issue |
3 |
| container_start_page |
493 |
| container_title |
The Journal of cell biology |
| container_volume |
119 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792347965290971139 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T18:03:40.133Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Identification+of+programmed+cell+death+in+situ+via+specific+labeling+of+nuclear+DNA+fragmentation.&rft.date=1992-11-01&genre=article&issn=1540-8140&volume=119&issue=3&spage=493&epage=501&pages=493-501&jtitle=The+Journal+of+cell+biology&atitle=Identification+of+programmed+cell+death+in+situ+via+specific+labeling+of+nuclear+DNA+fragmentation.&aulast=Ben-Sasson&aufirst=S+A&rft_id=info%3Adoi%2F10.1083%2Fjcb.119.3.493&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792347965290971139 |
| author | Gavrieli, Y, Sherman, Y, Ben-Sasson, S A |
| author_facet | Gavrieli, Y, Sherman, Y, Ben-Sasson, S A, Gavrieli, Y, Sherman, Y, Ben-Sasson, S A |
| author_sort | gavrieli, y |
| container_issue | 3 |
| container_start_page | 493 |
| container_title | The Journal of cell biology |
| container_volume | 119 |
| description | <jats:p>Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxy-transferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.</jats:p> |
| doi_str_mv | 10.1083/jcb.119.3.493 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTE5LjMuNDkz |
| imprint | Rockefeller University Press, 1992 |
| imprint_str_mv | Rockefeller University Press, 1992 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14 |
| issn | 0021-9525, 1540-8140 |
| issn_str_mv | 0021-9525, 1540-8140 |
| language | English |
| last_indexed | 2024-03-01T18:03:40.133Z |
| match_str | gavrieli1992identificationofprogrammedcelldeathinsituviaspecificlabelingofnucleardnafragmentation |
| mega_collection | Rockefeller University Press (CrossRef) |
| physical | 493-501 |
| publishDate | 1992 |
| publishDateSort | 1992 |
| publisher | Rockefeller University Press |
| record_format | ai |
| recordtype | ai |
| series | The Journal of cell biology |
| source_id | 49 |
| spelling | Gavrieli, Y Sherman, Y Ben-Sasson, S A 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.119.3.493 <jats:p>Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxy-transferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.</jats:p> Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. The Journal of cell biology |
| spellingShingle | Gavrieli, Y, Sherman, Y, Ben-Sasson, S A, The Journal of cell biology, Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation., Cell Biology |
| title | Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_full | Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_fullStr | Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_full_unstemmed | Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_short | Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| title_sort | identification of programmed cell death in situ via specific labeling of nuclear dna fragmentation. |
| title_unstemmed | Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1083/jcb.119.3.493 |