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70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.
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| Zeitschriftentitel: | The Journal of cell biology |
|---|---|
| Personen und Körperschaften: | , , |
| In: | The Journal of cell biology, 107, 1988, 6, S. 2051-2057 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Rockefeller University Press
|
| Schlagwörter: |
| author_facet |
Murakami, H Pain, D Blobel, G Murakami, H Pain, D Blobel, G |
|---|---|
| author |
Murakami, H Pain, D Blobel, G |
| spellingShingle |
Murakami, H Pain, D Blobel, G The Journal of cell biology 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. Cell Biology |
| author_sort |
murakami, h |
| spelling |
Murakami, H Pain, D Blobel, G 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.107.6.2051 <jats:p>We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.</jats:p> 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. The Journal of cell biology |
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Rockefeller University Press, 1988 |
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Rockefeller University Press, 1988 |
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0021-9525 1540-8140 |
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1988 |
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The Journal of cell biology |
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49 |
| title |
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_unstemmed |
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_full |
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_fullStr |
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_full_unstemmed |
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_short |
70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_sort |
70-kd heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| topic |
Cell Biology |
| url |
http://dx.doi.org/10.1083/jcb.107.6.2051 |
| publishDate |
1988 |
| physical |
2051-2057 |
| description |
<jats:p>We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.</jats:p> |
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| author | Murakami, H, Pain, D, Blobel, G |
| author_facet | Murakami, H, Pain, D, Blobel, G, Murakami, H, Pain, D, Blobel, G |
| author_sort | murakami, h |
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| description | <jats:p>We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.</jats:p> |
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| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA4My9qY2IuMTA3LjYuMjA1MQ |
| imprint | Rockefeller University Press, 1988 |
| imprint_str_mv | Rockefeller University Press, 1988 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1 |
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| physical | 2051-2057 |
| publishDate | 1988 |
| publishDateSort | 1988 |
| publisher | Rockefeller University Press |
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| recordtype | ai |
| series | The Journal of cell biology |
| source_id | 49 |
| spelling | Murakami, H Pain, D Blobel, G 0021-9525 1540-8140 Rockefeller University Press Cell Biology http://dx.doi.org/10.1083/jcb.107.6.2051 <jats:p>We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.</jats:p> 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. The Journal of cell biology |
| spellingShingle | Murakami, H, Pain, D, Blobel, G, The Journal of cell biology, 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria., Cell Biology |
| title | 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_full | 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_fullStr | 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_full_unstemmed | 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_short | 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_sort | 70-kd heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| title_unstemmed | 70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria. |
| topic | Cell Biology |
| url | http://dx.doi.org/10.1083/jcb.107.6.2051 |