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A Ds insertion alters the nuclear localization of the maize transcriptional activator R.
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 93, 1996, 15, S. 7816-7820 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Liu, Y Alleman, M Wessler, S R Liu, Y Alleman, M Wessler, S R |
|---|---|
| author |
Liu, Y Alleman, M Wessler, S R |
| spellingShingle |
Liu, Y Alleman, M Wessler, S R Proceedings of the National Academy of Sciences A Ds insertion alters the nuclear localization of the maize transcriptional activator R. Multidisciplinary |
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liu, y |
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Liu, Y Alleman, M Wessler, S R 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.93.15.7816 <jats:p>The R-sc gene of maize is a member of the R gene family of transcriptional activators that regulate anthocyanin biosynthesis. A derivative of R-sc, r-m9 conditions a reduced level of aleurone pigmentation due to the presence of a 2.1-kb Ds insertion near the 3' end of the coding region. Excision of Ds from r-m9 leaves a 7-bp insertion in the darker but still mutant v24 derivative. Both the 7-bp insertion in v24 and the 2.1-kb Ds in r-m9 are predicted to truncate their respective R proteins proximal to the carboxyl terminus, which was shown previously to contain one of three nuclear localization sequences. We find that the reduced expression of r-m9 and v24 are not due to mRNA or protein instability, but most likely reflect the inefficient localization of truncated R proteins to the nucleus. To our knowledge this is the first example of a transposable element insertion that alters gene expression by affecting nuclear localization. In addition, our data indicate that the carboxyl terminus of the R protein is far more important than previously suspected and illustrates the utility of natural mutations for defining functional domains in proteins.</jats:p> A Ds insertion alters the nuclear localization of the maize transcriptional activator R. Proceedings of the National Academy of Sciences |
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Proceedings of the National Academy of Sciences, 1996 |
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Proceedings of the National Academy of Sciences, 1996 |
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1996 |
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| title |
A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_unstemmed |
A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_full |
A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_fullStr |
A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_full_unstemmed |
A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_short |
A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_sort |
a ds insertion alters the nuclear localization of the maize transcriptional activator r. |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.93.15.7816 |
| publishDate |
1996 |
| physical |
7816-7820 |
| description |
<jats:p>The R-sc gene of maize is a member of the R gene family of transcriptional activators that regulate anthocyanin biosynthesis. A derivative of R-sc, r-m9 conditions a reduced level of aleurone pigmentation due to the presence of a 2.1-kb Ds insertion near the 3' end of the coding region. Excision of Ds from r-m9 leaves a 7-bp insertion in the darker but still mutant v24 derivative. Both the 7-bp insertion in v24 and the 2.1-kb Ds in r-m9 are predicted to truncate their respective R proteins proximal to the carboxyl terminus, which was shown previously to contain one of three nuclear localization sequences. We find that the reduced expression of r-m9 and v24 are not due to mRNA or protein instability, but most likely reflect the inefficient localization of truncated R proteins to the nucleus. To our knowledge this is the first example of a transposable element insertion that alters gene expression by affecting nuclear localization. In addition, our data indicate that the carboxyl terminus of the R protein is far more important than previously suspected and illustrates the utility of natural mutations for defining functional domains in proteins.</jats:p> |
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| author | Liu, Y, Alleman, M, Wessler, S R |
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| description | <jats:p>The R-sc gene of maize is a member of the R gene family of transcriptional activators that regulate anthocyanin biosynthesis. A derivative of R-sc, r-m9 conditions a reduced level of aleurone pigmentation due to the presence of a 2.1-kb Ds insertion near the 3' end of the coding region. Excision of Ds from r-m9 leaves a 7-bp insertion in the darker but still mutant v24 derivative. Both the 7-bp insertion in v24 and the 2.1-kb Ds in r-m9 are predicted to truncate their respective R proteins proximal to the carboxyl terminus, which was shown previously to contain one of three nuclear localization sequences. We find that the reduced expression of r-m9 and v24 are not due to mRNA or protein instability, but most likely reflect the inefficient localization of truncated R proteins to the nucleus. To our knowledge this is the first example of a transposable element insertion that alters gene expression by affecting nuclear localization. In addition, our data indicate that the carboxyl terminus of the R protein is far more important than previously suspected and illustrates the utility of natural mutations for defining functional domains in proteins.</jats:p> |
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| imprint | Proceedings of the National Academy of Sciences, 1996 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 1996 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Liu, Y Alleman, M Wessler, S R 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.93.15.7816 <jats:p>The R-sc gene of maize is a member of the R gene family of transcriptional activators that regulate anthocyanin biosynthesis. A derivative of R-sc, r-m9 conditions a reduced level of aleurone pigmentation due to the presence of a 2.1-kb Ds insertion near the 3' end of the coding region. Excision of Ds from r-m9 leaves a 7-bp insertion in the darker but still mutant v24 derivative. Both the 7-bp insertion in v24 and the 2.1-kb Ds in r-m9 are predicted to truncate their respective R proteins proximal to the carboxyl terminus, which was shown previously to contain one of three nuclear localization sequences. We find that the reduced expression of r-m9 and v24 are not due to mRNA or protein instability, but most likely reflect the inefficient localization of truncated R proteins to the nucleus. To our knowledge this is the first example of a transposable element insertion that alters gene expression by affecting nuclear localization. In addition, our data indicate that the carboxyl terminus of the R protein is far more important than previously suspected and illustrates the utility of natural mutations for defining functional domains in proteins.</jats:p> A Ds insertion alters the nuclear localization of the maize transcriptional activator R. Proceedings of the National Academy of Sciences |
| spellingShingle | Liu, Y, Alleman, M, Wessler, S R, Proceedings of the National Academy of Sciences, A Ds insertion alters the nuclear localization of the maize transcriptional activator R., Multidisciplinary |
| title | A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_full | A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_fullStr | A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_full_unstemmed | A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_short | A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| title_sort | a ds insertion alters the nuclear localization of the maize transcriptional activator r. |
| title_unstemmed | A Ds insertion alters the nuclear localization of the maize transcriptional activator R. |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.93.15.7816 |