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CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
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| Personen und Körperschaften: | , , , , , , , , |
| In: | Proceedings of the National Academy of Sciences, 98, 2001, 3, S. 1160-1165 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Fling, Steven P. Sutherland, R. Alec Steele, Lisa N. Hess, Bruce D'Orazio, Sarah E. F. Maisonneuve, Jean-François Lampe, Mary F. Probst, Peter Starnbach, Michael N. Fling, Steven P. Sutherland, R. Alec Steele, Lisa N. Hess, Bruce D'Orazio, Sarah E. F. Maisonneuve, Jean-François Lampe, Mary F. Probst, Peter Starnbach, Michael N. |
|---|---|
| author |
Fling, Steven P. Sutherland, R. Alec Steele, Lisa N. Hess, Bruce D'Orazio, Sarah E. F. Maisonneuve, Jean-François Lampe, Mary F. Probst, Peter Starnbach, Michael N. |
| spellingShingle |
Fling, Steven P. Sutherland, R. Alec Steele, Lisa N. Hess, Bruce D'Orazio, Sarah E. F. Maisonneuve, Jean-François Lampe, Mary F. Probst, Peter Starnbach, Michael N. Proceedings of the National Academy of Sciences CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis Multidisciplinary |
| author_sort |
fling, steven p. |
| spelling |
Fling, Steven P. Sutherland, R. Alec Steele, Lisa N. Hess, Bruce D'Orazio, Sarah E. F. Maisonneuve, Jean-François Lampe, Mary F. Probst, Peter Starnbach, Michael N. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.98.3.1160 <jats:p>During infection with<jats:italic>Chlamydia trachomatis</jats:italic>, CD8<jats:sup>+</jats:sup>T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8<jats:sup>+</jats:sup>T cells recognize antigens processed from cytosolic proteins, the<jats:italic>Chlamydia</jats:italic>antigens recognized by these CD8<jats:sup>+</jats:sup>T cells very likely have access to the host cell cytoplasm during infection. The identity of these<jats:italic>C. trachomatis</jats:italic>proteins has remained elusive, even though their localization suggests they may play important roles in the biology of the organism. Here we use a retroviral expression system to identify Cap1, a 31-kDa protein from<jats:italic>C. trachomatis</jats:italic>recognized by protective CD8<jats:sup>+</jats:sup>T cells. Cap1 contains no strong homology to any known protein. Immunofluorescence microscopy by using Cap1-specific antibody demonstrates that this protein is localized to the vacuolar membrane. Cap1 is virtually identical among the human<jats:italic>C. trachomatis</jats:italic>serovars, suggesting that a vaccine incorporating Cap1 might enable the vaccine to protect against all<jats:italic>C. trachomatis</jats:italic>serovars. The identification of proteins such as Cap1 that associate with the inclusion membrane will be required to fully understand the interaction of<jats:italic>C. trachomatis</jats:italic>with its host cell.</jats:p> CD8<sup>+</sup>T cells recognize an inclusion membrane-associated protein from the vacuolar pathogen<i>Chlamydia trachomatis</i> Proceedings of the National Academy of Sciences |
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| title |
CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_unstemmed |
CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_full |
CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_fullStr |
CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_full_unstemmed |
CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_short |
CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_sort |
cd8<sup>+</sup>t cells recognize an inclusion membrane-associated protein from the vacuolar pathogen<i>chlamydia trachomatis</i> |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.98.3.1160 |
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2001 |
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1160-1165 |
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<jats:p>During infection with<jats:italic>Chlamydia trachomatis</jats:italic>, CD8<jats:sup>+</jats:sup>T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8<jats:sup>+</jats:sup>T cells recognize antigens processed from cytosolic proteins, the<jats:italic>Chlamydia</jats:italic>antigens recognized by these CD8<jats:sup>+</jats:sup>T cells very likely have access to the host cell cytoplasm during infection. The identity of these<jats:italic>C. trachomatis</jats:italic>proteins has remained elusive, even though their localization suggests they may play important roles in the biology of the organism. Here we use a retroviral expression system to identify Cap1, a 31-kDa protein from<jats:italic>C. trachomatis</jats:italic>recognized by protective CD8<jats:sup>+</jats:sup>T cells. Cap1 contains no strong homology to any known protein. Immunofluorescence microscopy by using Cap1-specific antibody demonstrates that this protein is localized to the vacuolar membrane. Cap1 is virtually identical among the human<jats:italic>C. trachomatis</jats:italic>serovars, suggesting that a vaccine incorporating Cap1 might enable the vaccine to protect against all<jats:italic>C. trachomatis</jats:italic>serovars. The identification of proteins such as Cap1 that associate with the inclusion membrane will be required to fully understand the interaction of<jats:italic>C. trachomatis</jats:italic>with its host cell.</jats:p> |
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| author | Fling, Steven P., Sutherland, R. Alec, Steele, Lisa N., Hess, Bruce, D'Orazio, Sarah E. F., Maisonneuve, Jean-François, Lampe, Mary F., Probst, Peter, Starnbach, Michael N. |
| author_facet | Fling, Steven P., Sutherland, R. Alec, Steele, Lisa N., Hess, Bruce, D'Orazio, Sarah E. F., Maisonneuve, Jean-François, Lampe, Mary F., Probst, Peter, Starnbach, Michael N., Fling, Steven P., Sutherland, R. Alec, Steele, Lisa N., Hess, Bruce, D'Orazio, Sarah E. F., Maisonneuve, Jean-François, Lampe, Mary F., Probst, Peter, Starnbach, Michael N. |
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| description | <jats:p>During infection with<jats:italic>Chlamydia trachomatis</jats:italic>, CD8<jats:sup>+</jats:sup>T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8<jats:sup>+</jats:sup>T cells recognize antigens processed from cytosolic proteins, the<jats:italic>Chlamydia</jats:italic>antigens recognized by these CD8<jats:sup>+</jats:sup>T cells very likely have access to the host cell cytoplasm during infection. The identity of these<jats:italic>C. trachomatis</jats:italic>proteins has remained elusive, even though their localization suggests they may play important roles in the biology of the organism. Here we use a retroviral expression system to identify Cap1, a 31-kDa protein from<jats:italic>C. trachomatis</jats:italic>recognized by protective CD8<jats:sup>+</jats:sup>T cells. Cap1 contains no strong homology to any known protein. Immunofluorescence microscopy by using Cap1-specific antibody demonstrates that this protein is localized to the vacuolar membrane. Cap1 is virtually identical among the human<jats:italic>C. trachomatis</jats:italic>serovars, suggesting that a vaccine incorporating Cap1 might enable the vaccine to protect against all<jats:italic>C. trachomatis</jats:italic>serovars. The identification of proteins such as Cap1 that associate with the inclusion membrane will be required to fully understand the interaction of<jats:italic>C. trachomatis</jats:italic>with its host cell.</jats:p> |
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| imprint_str_mv | Proceedings of the National Academy of Sciences, 2001 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Fling, Steven P. Sutherland, R. Alec Steele, Lisa N. Hess, Bruce D'Orazio, Sarah E. F. Maisonneuve, Jean-François Lampe, Mary F. Probst, Peter Starnbach, Michael N. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.98.3.1160 <jats:p>During infection with<jats:italic>Chlamydia trachomatis</jats:italic>, CD8<jats:sup>+</jats:sup>T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8<jats:sup>+</jats:sup>T cells recognize antigens processed from cytosolic proteins, the<jats:italic>Chlamydia</jats:italic>antigens recognized by these CD8<jats:sup>+</jats:sup>T cells very likely have access to the host cell cytoplasm during infection. The identity of these<jats:italic>C. trachomatis</jats:italic>proteins has remained elusive, even though their localization suggests they may play important roles in the biology of the organism. Here we use a retroviral expression system to identify Cap1, a 31-kDa protein from<jats:italic>C. trachomatis</jats:italic>recognized by protective CD8<jats:sup>+</jats:sup>T cells. Cap1 contains no strong homology to any known protein. Immunofluorescence microscopy by using Cap1-specific antibody demonstrates that this protein is localized to the vacuolar membrane. Cap1 is virtually identical among the human<jats:italic>C. trachomatis</jats:italic>serovars, suggesting that a vaccine incorporating Cap1 might enable the vaccine to protect against all<jats:italic>C. trachomatis</jats:italic>serovars. The identification of proteins such as Cap1 that associate with the inclusion membrane will be required to fully understand the interaction of<jats:italic>C. trachomatis</jats:italic>with its host cell.</jats:p> CD8<sup>+</sup>T cells recognize an inclusion membrane-associated protein from the vacuolar pathogen<i>Chlamydia trachomatis</i> Proceedings of the National Academy of Sciences |
| spellingShingle | Fling, Steven P., Sutherland, R. Alec, Steele, Lisa N., Hess, Bruce, D'Orazio, Sarah E. F., Maisonneuve, Jean-François, Lampe, Mary F., Probst, Peter, Starnbach, Michael N., Proceedings of the National Academy of Sciences, CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis, Multidisciplinary |
| title | CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_full | CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_fullStr | CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_full_unstemmed | CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_short | CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| title_sort | cd8<sup>+</sup>t cells recognize an inclusion membrane-associated protein from the vacuolar pathogen<i>chlamydia trachomatis</i> |
| title_unstemmed | CD8+T cells recognize an inclusion membrane-associated protein from the vacuolar pathogenChlamydia trachomatis |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.98.3.1160 |