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Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patient...
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Proceedings of the National Academy of Sciences, 95, 1998, 13, S. 7568-7573 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Greten, Tim F. Slansky, Jill E. Kubota, Ryuji Soldan, Samantha S. Jaffee, Elizabeth M. Leist, Thomas P. Pardoll, Drew M. Jacobson, Steven Schneck, Jonathan P. Greten, Tim F. Slansky, Jill E. Kubota, Ryuji Soldan, Samantha S. Jaffee, Elizabeth M. Leist, Thomas P. Pardoll, Drew M. Jacobson, Steven Schneck, Jonathan P. |
|---|---|
| author |
Greten, Tim F. Slansky, Jill E. Kubota, Ryuji Soldan, Samantha S. Jaffee, Elizabeth M. Leist, Thomas P. Pardoll, Drew M. Jacobson, Steven Schneck, Jonathan P. |
| spellingShingle |
Greten, Tim F. Slansky, Jill E. Kubota, Ryuji Soldan, Samantha S. Jaffee, Elizabeth M. Leist, Thomas P. Pardoll, Drew M. Jacobson, Steven Schneck, Jonathan P. Proceedings of the National Academy of Sciences Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients Multidisciplinary |
| author_sort |
greten, tim f. |
| spelling |
Greten, Tim F. Slansky, Jill E. Kubota, Ryuji Soldan, Samantha S. Jaffee, Elizabeth M. Leist, Thomas P. Pardoll, Drew M. Jacobson, Steven Schneck, Jonathan P. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.95.13.7568 <jats:p>Human T lymphotropic virus type 1 (HTLV-1) -associated myelopathy/tropic spastic paraparesis is a demyelinating inflammatory neurologic disease associated with HTLV-1 infection. HTLV-1 Tax11–19-specific cytotoxic T cells have been isolated from HLA-A2-positive patients. We have used a peptide-loaded soluble HLA-A2–Ig complex to directly visualize HTLV-1 Tax11–19-specific T cells from peripheral blood and cerebrospinal fluid without<jats:italic>in vitro</jats:italic>stimulation. Five of six HTLV-1-associated myelopathy/tropic spastic paraparesis patients carried a significant number (up to 13.87%) of CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>lymphocytes specific for the HTLV-1 Tax11–19 peptide in their peripheral blood, which were not found in healthy controls. Simultaneous comparison of peripheral blood and cerebrospinal fluid from one patient revealed 2.5-fold more Tax11–19-specific T cells in the cerebrospinal fluid (23.7% vs. 9.4% in peripheral blood lymphocyte). Tax11–19-specific T cells were seen consistently over a 9-yr time course in one patient as far as 19 yrs after the onset of clinical symptoms. Further analysis of HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T lymphocytes in HAM/TSP patients showed different expression patterns of activation markers, intracellular TNF-α and γ-interferon depending on the severity of the disease. Thus, visualization of antigen-specific T cells demonstrates that HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T cells are activated, persist during the chronic phase of the disease, and accumulate in cerebrospinal fluid, showing their pivotal role in the pathogenesis of this neurologic disease.</jats:p> Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8<sup>+</sup>T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients Proceedings of the National Academy of Sciences |
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10.1073/pnas.95.13.7568 |
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Proceedings of the National Academy of Sciences, 1998 |
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Proceedings of the National Academy of Sciences, 1998 |
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0027-8424 1091-6490 |
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0027-8424 1091-6490 |
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1998 |
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Proceedings of the National Academy of Sciences |
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| title |
Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_unstemmed |
Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_full |
Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_fullStr |
Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_full_unstemmed |
Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_short |
Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_sort |
direct visualization of antigen-specific t cells: htlv-1 tax11–19- specific cd8<sup>+</sup>t cells are activated in peripheral blood and accumulate in cerebrospinal fluid from ham/tsp patients |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.95.13.7568 |
| publishDate |
1998 |
| physical |
7568-7573 |
| description |
<jats:p>Human T lymphotropic virus type 1 (HTLV-1) -associated myelopathy/tropic spastic paraparesis is a demyelinating inflammatory neurologic disease associated with HTLV-1 infection. HTLV-1 Tax11–19-specific cytotoxic T cells have been isolated from HLA-A2-positive patients. We have used a peptide-loaded soluble HLA-A2–Ig complex to directly visualize HTLV-1 Tax11–19-specific T cells from peripheral blood and cerebrospinal fluid without<jats:italic>in vitro</jats:italic>stimulation. Five of six HTLV-1-associated myelopathy/tropic spastic paraparesis patients carried a significant number (up to 13.87%) of CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>lymphocytes specific for the HTLV-1 Tax11–19 peptide in their peripheral blood, which were not found in healthy controls. Simultaneous comparison of peripheral blood and cerebrospinal fluid from one patient revealed 2.5-fold more Tax11–19-specific T cells in the cerebrospinal fluid (23.7% vs. 9.4% in peripheral blood lymphocyte). Tax11–19-specific T cells were seen consistently over a 9-yr time course in one patient as far as 19 yrs after the onset of clinical symptoms. Further analysis of HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T lymphocytes in HAM/TSP patients showed different expression patterns of activation markers, intracellular TNF-α and γ-interferon depending on the severity of the disease. Thus, visualization of antigen-specific T cells demonstrates that HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T cells are activated, persist during the chronic phase of the disease, and accumulate in cerebrospinal fluid, showing their pivotal role in the pathogenesis of this neurologic disease.</jats:p> |
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| author | Greten, Tim F., Slansky, Jill E., Kubota, Ryuji, Soldan, Samantha S., Jaffee, Elizabeth M., Leist, Thomas P., Pardoll, Drew M., Jacobson, Steven, Schneck, Jonathan P. |
| author_facet | Greten, Tim F., Slansky, Jill E., Kubota, Ryuji, Soldan, Samantha S., Jaffee, Elizabeth M., Leist, Thomas P., Pardoll, Drew M., Jacobson, Steven, Schneck, Jonathan P., Greten, Tim F., Slansky, Jill E., Kubota, Ryuji, Soldan, Samantha S., Jaffee, Elizabeth M., Leist, Thomas P., Pardoll, Drew M., Jacobson, Steven, Schneck, Jonathan P. |
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| description | <jats:p>Human T lymphotropic virus type 1 (HTLV-1) -associated myelopathy/tropic spastic paraparesis is a demyelinating inflammatory neurologic disease associated with HTLV-1 infection. HTLV-1 Tax11–19-specific cytotoxic T cells have been isolated from HLA-A2-positive patients. We have used a peptide-loaded soluble HLA-A2–Ig complex to directly visualize HTLV-1 Tax11–19-specific T cells from peripheral blood and cerebrospinal fluid without<jats:italic>in vitro</jats:italic>stimulation. Five of six HTLV-1-associated myelopathy/tropic spastic paraparesis patients carried a significant number (up to 13.87%) of CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>lymphocytes specific for the HTLV-1 Tax11–19 peptide in their peripheral blood, which were not found in healthy controls. Simultaneous comparison of peripheral blood and cerebrospinal fluid from one patient revealed 2.5-fold more Tax11–19-specific T cells in the cerebrospinal fluid (23.7% vs. 9.4% in peripheral blood lymphocyte). Tax11–19-specific T cells were seen consistently over a 9-yr time course in one patient as far as 19 yrs after the onset of clinical symptoms. Further analysis of HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T lymphocytes in HAM/TSP patients showed different expression patterns of activation markers, intracellular TNF-α and γ-interferon depending on the severity of the disease. Thus, visualization of antigen-specific T cells demonstrates that HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T cells are activated, persist during the chronic phase of the disease, and accumulate in cerebrospinal fluid, showing their pivotal role in the pathogenesis of this neurologic disease.</jats:p> |
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| imprint | Proceedings of the National Academy of Sciences, 1998 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 1998 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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| match_str | greten1998directvisualizationofantigenspecifictcellshtlv1tax1119specificcd8tcellsareactivatedinperipheralbloodandaccumulateincerebrospinalfluidfromhamtsppatients |
| mega_collection | Proceedings of the National Academy of Sciences (CrossRef) |
| physical | 7568-7573 |
| publishDate | 1998 |
| publishDateSort | 1998 |
| publisher | Proceedings of the National Academy of Sciences |
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| series | Proceedings of the National Academy of Sciences |
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| spelling | Greten, Tim F. Slansky, Jill E. Kubota, Ryuji Soldan, Samantha S. Jaffee, Elizabeth M. Leist, Thomas P. Pardoll, Drew M. Jacobson, Steven Schneck, Jonathan P. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.95.13.7568 <jats:p>Human T lymphotropic virus type 1 (HTLV-1) -associated myelopathy/tropic spastic paraparesis is a demyelinating inflammatory neurologic disease associated with HTLV-1 infection. HTLV-1 Tax11–19-specific cytotoxic T cells have been isolated from HLA-A2-positive patients. We have used a peptide-loaded soluble HLA-A2–Ig complex to directly visualize HTLV-1 Tax11–19-specific T cells from peripheral blood and cerebrospinal fluid without<jats:italic>in vitro</jats:italic>stimulation. Five of six HTLV-1-associated myelopathy/tropic spastic paraparesis patients carried a significant number (up to 13.87%) of CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>lymphocytes specific for the HTLV-1 Tax11–19 peptide in their peripheral blood, which were not found in healthy controls. Simultaneous comparison of peripheral blood and cerebrospinal fluid from one patient revealed 2.5-fold more Tax11–19-specific T cells in the cerebrospinal fluid (23.7% vs. 9.4% in peripheral blood lymphocyte). Tax11–19-specific T cells were seen consistently over a 9-yr time course in one patient as far as 19 yrs after the onset of clinical symptoms. Further analysis of HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T lymphocytes in HAM/TSP patients showed different expression patterns of activation markers, intracellular TNF-α and γ-interferon depending on the severity of the disease. Thus, visualization of antigen-specific T cells demonstrates that HTLV-1 Tax11–19-specific CD8<jats:sup><jats:italic>+</jats:italic></jats:sup>T cells are activated, persist during the chronic phase of the disease, and accumulate in cerebrospinal fluid, showing their pivotal role in the pathogenesis of this neurologic disease.</jats:p> Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8<sup>+</sup>T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients Proceedings of the National Academy of Sciences |
| spellingShingle | Greten, Tim F., Slansky, Jill E., Kubota, Ryuji, Soldan, Samantha S., Jaffee, Elizabeth M., Leist, Thomas P., Pardoll, Drew M., Jacobson, Steven, Schneck, Jonathan P., Proceedings of the National Academy of Sciences, Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients, Multidisciplinary |
| title | Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_full | Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_fullStr | Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_full_unstemmed | Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_short | Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| title_sort | direct visualization of antigen-specific t cells: htlv-1 tax11–19- specific cd8<sup>+</sup>t cells are activated in peripheral blood and accumulate in cerebrospinal fluid from ham/tsp patients |
| title_unstemmed | Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8+T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.95.13.7568 |