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Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 94, 1997, 23, S. 12314-12319 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Malone, Cindy Sue Omori, Sidne A. Wall, Randolph Malone, Cindy Sue Omori, Sidne A. Wall, Randolph |
|---|---|
| author |
Malone, Cindy Sue Omori, Sidne A. Wall, Randolph |
| spellingShingle |
Malone, Cindy Sue Omori, Sidne A. Wall, Randolph Proceedings of the National Academy of Sciences Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific Multidisciplinary |
| author_sort |
malone, cindy sue |
| spelling |
Malone, Cindy Sue Omori, Sidne A. Wall, Randolph 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.94.23.12314 <jats:p>The murine B29 (Igβ) promoter is B cell specific and contains essential SP1, ETS, OCT, and Ikaros motifs. Flanking 5′ DNA sequences inhibit B29 promoter activity, suggesting this region contains silencer elements. Two adjacent 5′ DNA segments repress transcription by the murine B29 promoter in a position- and orientation-independent manner, analogous to known silencers. Both these 5′ segments also inhibit transcription by several heterologous promoters in B cells, including mb-1, c-<jats:italic>fos,</jats:italic>and human B29. These 5′ segments also inhibit transcription by the c-<jats:italic>fos</jats:italic>promoter in T cells suggesting they are not B cell-specific elements. DNase I footprint analyses show an approximately 70-bp protected region overlapping the boundary between the two negative regulatory DNA segments and corresponding to binding sites for at least two different DNA-binding proteins. Within this footprint, two unrelated 30-bp cis-acting DNA motifs (designated TOAD and FROG) function as position- and orientation-independent silencers when located directly 5′ of the murine B29 promoter. These two silencer motifs act cooperatively to restrict the transcriptional activity of the B29 promoter. Neither of these motifs resembles any known silencers. Mutagenesis of the TOAD and FROG motifs in their respective 5′ DNA segments eliminates the silencing activity of these upstream regions, indicating these two motifs as the principal B29 silencer elements within these regions.</jats:p> Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific Proceedings of the National Academy of Sciences |
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| title |
Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_unstemmed |
Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_full |
Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_fullStr |
Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_full_unstemmed |
Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_short |
Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_sort |
silencer elements controlling the b29 (igβ) promoter are neither promoter- nor cell-type-specific |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.94.23.12314 |
| publishDate |
1997 |
| physical |
12314-12319 |
| description |
<jats:p>The murine B29 (Igβ) promoter is B cell specific and contains essential SP1, ETS, OCT, and Ikaros motifs. Flanking 5′ DNA sequences inhibit B29 promoter activity, suggesting this region contains silencer elements. Two adjacent 5′ DNA segments repress transcription by the murine B29 promoter in a position- and orientation-independent manner, analogous to known silencers. Both these 5′ segments also inhibit transcription by several heterologous promoters in B cells, including mb-1, c-<jats:italic>fos,</jats:italic>and human B29. These 5′ segments also inhibit transcription by the c-<jats:italic>fos</jats:italic>promoter in T cells suggesting they are not B cell-specific elements. DNase I footprint analyses show an approximately 70-bp protected region overlapping the boundary between the two negative regulatory DNA segments and corresponding to binding sites for at least two different DNA-binding proteins. Within this footprint, two unrelated 30-bp cis-acting DNA motifs (designated TOAD and FROG) function as position- and orientation-independent silencers when located directly 5′ of the murine B29 promoter. These two silencer motifs act cooperatively to restrict the transcriptional activity of the B29 promoter. Neither of these motifs resembles any known silencers. Mutagenesis of the TOAD and FROG motifs in their respective 5′ DNA segments eliminates the silencing activity of these upstream regions, indicating these two motifs as the principal B29 silencer elements within these regions.</jats:p> |
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| author | Malone, Cindy Sue, Omori, Sidne A., Wall, Randolph |
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| description | <jats:p>The murine B29 (Igβ) promoter is B cell specific and contains essential SP1, ETS, OCT, and Ikaros motifs. Flanking 5′ DNA sequences inhibit B29 promoter activity, suggesting this region contains silencer elements. Two adjacent 5′ DNA segments repress transcription by the murine B29 promoter in a position- and orientation-independent manner, analogous to known silencers. Both these 5′ segments also inhibit transcription by several heterologous promoters in B cells, including mb-1, c-<jats:italic>fos,</jats:italic>and human B29. These 5′ segments also inhibit transcription by the c-<jats:italic>fos</jats:italic>promoter in T cells suggesting they are not B cell-specific elements. DNase I footprint analyses show an approximately 70-bp protected region overlapping the boundary between the two negative regulatory DNA segments and corresponding to binding sites for at least two different DNA-binding proteins. Within this footprint, two unrelated 30-bp cis-acting DNA motifs (designated TOAD and FROG) function as position- and orientation-independent silencers when located directly 5′ of the murine B29 promoter. These two silencer motifs act cooperatively to restrict the transcriptional activity of the B29 promoter. Neither of these motifs resembles any known silencers. Mutagenesis of the TOAD and FROG motifs in their respective 5′ DNA segments eliminates the silencing activity of these upstream regions, indicating these two motifs as the principal B29 silencer elements within these regions.</jats:p> |
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| spelling | Malone, Cindy Sue Omori, Sidne A. Wall, Randolph 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.94.23.12314 <jats:p>The murine B29 (Igβ) promoter is B cell specific and contains essential SP1, ETS, OCT, and Ikaros motifs. Flanking 5′ DNA sequences inhibit B29 promoter activity, suggesting this region contains silencer elements. Two adjacent 5′ DNA segments repress transcription by the murine B29 promoter in a position- and orientation-independent manner, analogous to known silencers. Both these 5′ segments also inhibit transcription by several heterologous promoters in B cells, including mb-1, c-<jats:italic>fos,</jats:italic>and human B29. These 5′ segments also inhibit transcription by the c-<jats:italic>fos</jats:italic>promoter in T cells suggesting they are not B cell-specific elements. DNase I footprint analyses show an approximately 70-bp protected region overlapping the boundary between the two negative regulatory DNA segments and corresponding to binding sites for at least two different DNA-binding proteins. Within this footprint, two unrelated 30-bp cis-acting DNA motifs (designated TOAD and FROG) function as position- and orientation-independent silencers when located directly 5′ of the murine B29 promoter. These two silencer motifs act cooperatively to restrict the transcriptional activity of the B29 promoter. Neither of these motifs resembles any known silencers. Mutagenesis of the TOAD and FROG motifs in their respective 5′ DNA segments eliminates the silencing activity of these upstream regions, indicating these two motifs as the principal B29 silencer elements within these regions.</jats:p> Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific Proceedings of the National Academy of Sciences |
| spellingShingle | Malone, Cindy Sue, Omori, Sidne A., Wall, Randolph, Proceedings of the National Academy of Sciences, Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific, Multidisciplinary |
| title | Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_full | Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_fullStr | Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_full_unstemmed | Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_short | Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| title_sort | silencer elements controlling the b29 (igβ) promoter are neither promoter- nor cell-type-specific |
| title_unstemmed | Silencer elements controlling the B29 (Igβ) promoter are neither promoter- nor cell-type-specific |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.94.23.12314 |