Eintrag weiter verarbeiten
Verfügbar über Online-Ressource

A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.

Gespeichert in:

Bibliographische Detailangaben
Zeitschriftentitel: Proceedings of the National Academy of Sciences
Personen und Körperschaften: Murphy, S N, Miller, R J
In: Proceedings of the National Academy of Sciences, 85, 1988, 22, S. 8737-8741
Format: E-Article
Sprache: Englisch
veröffentlicht:
Proceedings of the National Academy of Sciences
Schlagwörter:
Multidisciplinary
author_facet Murphy, S N
Miller, R J
Murphy, S N
Miller, R J
author Murphy, S N
Miller, R J
spellingShingle Murphy, S N
Miller, R J
Proceedings of the National Academy of Sciences
A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
Multidisciplinary
author_sort murphy, s n
spelling Murphy, S N Miller, R J 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.85.22.8737 <jats:p>We investigated the effect of various excitatory amino acids on intracellular free Ca2+ concentration ( [Ca2+]i) in single mouse hippocampal neurons in vitro by using the Ca2+-sensitive dye fura-2. In normal physiological solution, glutamate, kainate, N-methyl-D-aspartate, and quisqualate all produced increases in [Ca2+]i. When all extracellular Ca2+ was removed, kainate and N-methyl-D-aspartate were completely ineffective, but quisqualate and glutamate were able to produce a spike-like Ca2+ transient, presumably reflecting the release of Ca2+ from intracellular stores. Ca2+ transients of similar shape could also be produced by the alpha 1-adrenergic agonist phenylephrine. After the production of a Ca2+ transient a second addition of quisqualate was ineffective unless intracellular stores were refilled by loading the cell with Ca2+ following depolarization in Ca2+-containing medium. None of the conventional excitatory amino acid receptor antagonists inhibited the Ca2+-mobilizing effects of quisqualate. Furthermore alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) was unable to produce Ca2+ mobilization in Ca2+-free medium, although it could produce Ca2+ influx in Ca2+-containing medium. Thus, glutamate can produce mobilization of Ca2+ from intracellular stores in hippocampal neurons by acting on a quisqualate-sensitive but AMPA-insensitive receptor. This receptor is therefore distinct from the quisqualate receptor that produces cell depolarization. The possibility that this Ca2+-mobilizing effect is mediated by inositol triphosphate production is discussed.</jats:p> A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons. Proceedings of the National Academy of Sciences
doi_str_mv 10.1073/pnas.85.22.8737
facet_avail Online
Free
format ElectronicArticle
fullrecord blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjg1LjIyLjg3Mzc
id ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjg1LjIyLjg3Mzc
institution DE-Pl11
DE-Rs1
DE-105
DE-14
DE-Ch1
DE-L229
DE-D275
DE-Bn3
DE-Brt1
DE-Zwi2
DE-D161
DE-Gla1
DE-Zi4
DE-15
imprint Proceedings of the National Academy of Sciences, 1988
imprint_str_mv Proceedings of the National Academy of Sciences, 1988
issn 0027-8424
1091-6490
issn_str_mv 0027-8424
1091-6490
language English
mega_collection Proceedings of the National Academy of Sciences (CrossRef)
match_str murphy1988aglutamatereceptorregulatesca2mobilizationinhippocampalneurons
publishDateSort 1988
publisher Proceedings of the National Academy of Sciences
recordtype ai
record_format ai
series Proceedings of the National Academy of Sciences
source_id 49
title A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_unstemmed A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_full A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_fullStr A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_full_unstemmed A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_short A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_sort a glutamate receptor regulates ca2+ mobilization in hippocampal neurons.
topic Multidisciplinary
url http://dx.doi.org/10.1073/pnas.85.22.8737
publishDate 1988
physical 8737-8741
description <jats:p>We investigated the effect of various excitatory amino acids on intracellular free Ca2+ concentration ( [Ca2+]i) in single mouse hippocampal neurons in vitro by using the Ca2+-sensitive dye fura-2. In normal physiological solution, glutamate, kainate, N-methyl-D-aspartate, and quisqualate all produced increases in [Ca2+]i. When all extracellular Ca2+ was removed, kainate and N-methyl-D-aspartate were completely ineffective, but quisqualate and glutamate were able to produce a spike-like Ca2+ transient, presumably reflecting the release of Ca2+ from intracellular stores. Ca2+ transients of similar shape could also be produced by the alpha 1-adrenergic agonist phenylephrine. After the production of a Ca2+ transient a second addition of quisqualate was ineffective unless intracellular stores were refilled by loading the cell with Ca2+ following depolarization in Ca2+-containing medium. None of the conventional excitatory amino acid receptor antagonists inhibited the Ca2+-mobilizing effects of quisqualate. Furthermore alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) was unable to produce Ca2+ mobilization in Ca2+-free medium, although it could produce Ca2+ influx in Ca2+-containing medium. Thus, glutamate can produce mobilization of Ca2+ from intracellular stores in hippocampal neurons by acting on a quisqualate-sensitive but AMPA-insensitive receptor. This receptor is therefore distinct from the quisqualate receptor that produces cell depolarization. The possibility that this Ca2+-mobilizing effect is mediated by inositol triphosphate production is discussed.</jats:p>
container_issue 22
container_start_page 8737
container_title Proceedings of the National Academy of Sciences
container_volume 85
format_de105 Article, E-Article
format_de14 Article, E-Article
format_de15 Article, E-Article
format_de520 Article, E-Article
format_de540 Article, E-Article
format_dech1 Article, E-Article
format_ded117 Article, E-Article
format_degla1 E-Article
format_del152 Buch
format_del189 Article, E-Article
format_dezi4 Article
format_dezwi2 Article, E-Article
format_finc Article, E-Article
format_nrw Article, E-Article
_version_ 1792337173408645127
geogr_code not assigned
last_indexed 2024-03-01T15:12:08.228Z
geogr_code_person not assigned
openURL url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=A+glutamate+receptor+regulates+Ca2%2B+mobilization+in+hippocampal+neurons.&rft.date=1988-11-01&genre=article&issn=1091-6490&volume=85&issue=22&spage=8737&epage=8741&pages=8737-8741&jtitle=Proceedings+of+the+National+Academy+of+Sciences&atitle=A+glutamate+receptor+regulates+Ca2%2B+mobilization+in+hippocampal+neurons.&aulast=Miller&aufirst=R+J&rft_id=info%3Adoi%2F10.1073%2Fpnas.85.22.8737&rft.language%5B0%5D=eng
SOLR
_version_ 1792337173408645127
author Murphy, S N, Miller, R J
author_facet Murphy, S N, Miller, R J, Murphy, S N, Miller, R J
author_sort murphy, s n
container_issue 22
container_start_page 8737
container_title Proceedings of the National Academy of Sciences
container_volume 85
description <jats:p>We investigated the effect of various excitatory amino acids on intracellular free Ca2+ concentration ( [Ca2+]i) in single mouse hippocampal neurons in vitro by using the Ca2+-sensitive dye fura-2. In normal physiological solution, glutamate, kainate, N-methyl-D-aspartate, and quisqualate all produced increases in [Ca2+]i. When all extracellular Ca2+ was removed, kainate and N-methyl-D-aspartate were completely ineffective, but quisqualate and glutamate were able to produce a spike-like Ca2+ transient, presumably reflecting the release of Ca2+ from intracellular stores. Ca2+ transients of similar shape could also be produced by the alpha 1-adrenergic agonist phenylephrine. After the production of a Ca2+ transient a second addition of quisqualate was ineffective unless intracellular stores were refilled by loading the cell with Ca2+ following depolarization in Ca2+-containing medium. None of the conventional excitatory amino acid receptor antagonists inhibited the Ca2+-mobilizing effects of quisqualate. Furthermore alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) was unable to produce Ca2+ mobilization in Ca2+-free medium, although it could produce Ca2+ influx in Ca2+-containing medium. Thus, glutamate can produce mobilization of Ca2+ from intracellular stores in hippocampal neurons by acting on a quisqualate-sensitive but AMPA-insensitive receptor. This receptor is therefore distinct from the quisqualate receptor that produces cell depolarization. The possibility that this Ca2+-mobilizing effect is mediated by inositol triphosphate production is discussed.</jats:p>
doi_str_mv 10.1073/pnas.85.22.8737
facet_avail Online, Free
format ElectronicArticle
format_de105 Article, E-Article
format_de14 Article, E-Article
format_de15 Article, E-Article
format_de520 Article, E-Article
format_de540 Article, E-Article
format_dech1 Article, E-Article
format_ded117 Article, E-Article
format_degla1 E-Article
format_del152 Buch
format_del189 Article, E-Article
format_dezi4 Article
format_dezwi2 Article, E-Article
format_finc Article, E-Article
format_nrw Article, E-Article
geogr_code not assigned
geogr_code_person not assigned
id ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjg1LjIyLjg3Mzc
imprint Proceedings of the National Academy of Sciences, 1988
imprint_str_mv Proceedings of the National Academy of Sciences, 1988
institution DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15
issn 0027-8424, 1091-6490
issn_str_mv 0027-8424, 1091-6490
language English
last_indexed 2024-03-01T15:12:08.228Z
match_str murphy1988aglutamatereceptorregulatesca2mobilizationinhippocampalneurons
mega_collection Proceedings of the National Academy of Sciences (CrossRef)
physical 8737-8741
publishDate 1988
publishDateSort 1988
publisher Proceedings of the National Academy of Sciences
record_format ai
recordtype ai
series Proceedings of the National Academy of Sciences
source_id 49
spelling Murphy, S N Miller, R J 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.85.22.8737 <jats:p>We investigated the effect of various excitatory amino acids on intracellular free Ca2+ concentration ( [Ca2+]i) in single mouse hippocampal neurons in vitro by using the Ca2+-sensitive dye fura-2. In normal physiological solution, glutamate, kainate, N-methyl-D-aspartate, and quisqualate all produced increases in [Ca2+]i. When all extracellular Ca2+ was removed, kainate and N-methyl-D-aspartate were completely ineffective, but quisqualate and glutamate were able to produce a spike-like Ca2+ transient, presumably reflecting the release of Ca2+ from intracellular stores. Ca2+ transients of similar shape could also be produced by the alpha 1-adrenergic agonist phenylephrine. After the production of a Ca2+ transient a second addition of quisqualate was ineffective unless intracellular stores were refilled by loading the cell with Ca2+ following depolarization in Ca2+-containing medium. None of the conventional excitatory amino acid receptor antagonists inhibited the Ca2+-mobilizing effects of quisqualate. Furthermore alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) was unable to produce Ca2+ mobilization in Ca2+-free medium, although it could produce Ca2+ influx in Ca2+-containing medium. Thus, glutamate can produce mobilization of Ca2+ from intracellular stores in hippocampal neurons by acting on a quisqualate-sensitive but AMPA-insensitive receptor. This receptor is therefore distinct from the quisqualate receptor that produces cell depolarization. The possibility that this Ca2+-mobilizing effect is mediated by inositol triphosphate production is discussed.</jats:p> A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons. Proceedings of the National Academy of Sciences
spellingShingle Murphy, S N, Miller, R J, Proceedings of the National Academy of Sciences, A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons., Multidisciplinary
title A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_full A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_fullStr A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_full_unstemmed A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_short A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
title_sort a glutamate receptor regulates ca2+ mobilization in hippocampal neurons.
title_unstemmed A glutamate receptor regulates Ca2+ mobilization in hippocampal neurons.
topic Multidisciplinary
url http://dx.doi.org/10.1073/pnas.85.22.8737