Eintrag weiter verarbeiten
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections.
Gespeichert in:
| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 84, 1987, 3, S. 658-662 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
De Benedetti, A Pytel, B A Baglioni, C De Benedetti, A Pytel, B A Baglioni, C |
|---|---|
| author |
De Benedetti, A Pytel, B A Baglioni, C |
| spellingShingle |
De Benedetti, A Pytel, B A Baglioni, C Proceedings of the National Academy of Sciences Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. Multidisciplinary |
| author_sort |
de benedetti, a |
| spelling |
De Benedetti, A Pytel, B A Baglioni, C 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.84.3.658 <jats:p>An expression vector was constructed that carries part of the human BK papovavirus with 0.5 kilobases of (2'-5')oligoadenylate (2-5A) synthetase cDNA inserted in inverted orientation downstream from the virion proteins (VP) promoter and the neomycin-resistance gene neo under the control of a simian virus 40 promoter. Cells transfected with this vector and selected for resistance to the neomycin derivative G418 synthesized RNA complementary to 2-5A synthetase mRNA. These cells lacked 2-5A synthetase activity, and the enzyme was not inducible by interferon. In contrast, 2-5A synthetase was induced in cells transfected with a control vector without the cDNA insert. Such cells were protected by interferon from RNA viruses, whereas cells lacking 2-5A synthetase were not protected from encephalomyocarditis virus, vesicular stomatitis virus, and Sindbis virus but were fully protected from influenza virus. These findings show that a high level of 2-5A synthetase is required for interferon-induced protection from the cytoplasmic RNA viruses tested.</jats:p> Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. Proceedings of the National Academy of Sciences |
| doi_str_mv |
10.1073/pnas.84.3.658 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjg0LjMuNjU4 |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjg0LjMuNjU4 |
| institution |
DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 |
| imprint |
Proceedings of the National Academy of Sciences, 1987 |
| imprint_str_mv |
Proceedings of the National Academy of Sciences, 1987 |
| issn |
0027-8424 1091-6490 |
| issn_str_mv |
0027-8424 1091-6490 |
| language |
English |
| mega_collection |
Proceedings of the National Academy of Sciences (CrossRef) |
| match_str |
debenedetti1987lossof25oligoadenylatesynthetaseactivitybyproductionofantisensernaresultsinlackofprotectionbyinterferonfromviralinfections |
| publishDateSort |
1987 |
| publisher |
Proceedings of the National Academy of Sciences |
| recordtype |
ai |
| record_format |
ai |
| series |
Proceedings of the National Academy of Sciences |
| source_id |
49 |
| title |
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_unstemmed |
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_full |
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_fullStr |
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_full_unstemmed |
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_short |
Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_sort |
loss of (2'-5')oligoadenylate synthetase activity by production of antisense rna results in lack of protection by interferon from viral infections. |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.84.3.658 |
| publishDate |
1987 |
| physical |
658-662 |
| description |
<jats:p>An expression vector was constructed that carries part of the human BK papovavirus with 0.5 kilobases of (2'-5')oligoadenylate (2-5A) synthetase cDNA inserted in inverted orientation downstream from the virion proteins (VP) promoter and the neomycin-resistance gene neo under the control of a simian virus 40 promoter. Cells transfected with this vector and selected for resistance to the neomycin derivative G418 synthesized RNA complementary to 2-5A synthetase mRNA. These cells lacked 2-5A synthetase activity, and the enzyme was not inducible by interferon. In contrast, 2-5A synthetase was induced in cells transfected with a control vector without the cDNA insert. Such cells were protected by interferon from RNA viruses, whereas cells lacking 2-5A synthetase were not protected from encephalomyocarditis virus, vesicular stomatitis virus, and Sindbis virus but were fully protected from influenza virus. These findings show that a high level of 2-5A synthetase is required for interferon-induced protection from the cytoplasmic RNA viruses tested.</jats:p> |
| container_issue |
3 |
| container_start_page |
658 |
| container_title |
Proceedings of the National Academy of Sciences |
| container_volume |
84 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792336644887543813 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:03:33.015Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Loss+of+%282%27-5%27%29oligoadenylate+synthetase+activity+by+production+of+antisense+RNA+results+in+lack+of+protection+by+interferon+from+viral+infections.&rft.date=1987-02-01&genre=article&issn=1091-6490&volume=84&issue=3&spage=658&epage=662&pages=658-662&jtitle=Proceedings+of+the+National+Academy+of+Sciences&atitle=Loss+of+%282%27-5%27%29oligoadenylate+synthetase+activity+by+production+of+antisense+RNA+results+in+lack+of+protection+by+interferon+from+viral+infections.&aulast=Baglioni&aufirst=C&rft_id=info%3Adoi%2F10.1073%2Fpnas.84.3.658&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792336644887543813 |
| author | De Benedetti, A, Pytel, B A, Baglioni, C |
| author_facet | De Benedetti, A, Pytel, B A, Baglioni, C, De Benedetti, A, Pytel, B A, Baglioni, C |
| author_sort | de benedetti, a |
| container_issue | 3 |
| container_start_page | 658 |
| container_title | Proceedings of the National Academy of Sciences |
| container_volume | 84 |
| description | <jats:p>An expression vector was constructed that carries part of the human BK papovavirus with 0.5 kilobases of (2'-5')oligoadenylate (2-5A) synthetase cDNA inserted in inverted orientation downstream from the virion proteins (VP) promoter and the neomycin-resistance gene neo under the control of a simian virus 40 promoter. Cells transfected with this vector and selected for resistance to the neomycin derivative G418 synthesized RNA complementary to 2-5A synthetase mRNA. These cells lacked 2-5A synthetase activity, and the enzyme was not inducible by interferon. In contrast, 2-5A synthetase was induced in cells transfected with a control vector without the cDNA insert. Such cells were protected by interferon from RNA viruses, whereas cells lacking 2-5A synthetase were not protected from encephalomyocarditis virus, vesicular stomatitis virus, and Sindbis virus but were fully protected from influenza virus. These findings show that a high level of 2-5A synthetase is required for interferon-induced protection from the cytoplasmic RNA viruses tested.</jats:p> |
| doi_str_mv | 10.1073/pnas.84.3.658 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjg0LjMuNjU4 |
| imprint | Proceedings of the National Academy of Sciences, 1987 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 1987 |
| institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161 |
| issn | 0027-8424, 1091-6490 |
| issn_str_mv | 0027-8424, 1091-6490 |
| language | English |
| last_indexed | 2024-03-01T15:03:33.015Z |
| match_str | debenedetti1987lossof25oligoadenylatesynthetaseactivitybyproductionofantisensernaresultsinlackofprotectionbyinterferonfromviralinfections |
| mega_collection | Proceedings of the National Academy of Sciences (CrossRef) |
| physical | 658-662 |
| publishDate | 1987 |
| publishDateSort | 1987 |
| publisher | Proceedings of the National Academy of Sciences |
| record_format | ai |
| recordtype | ai |
| series | Proceedings of the National Academy of Sciences |
| source_id | 49 |
| spelling | De Benedetti, A Pytel, B A Baglioni, C 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.84.3.658 <jats:p>An expression vector was constructed that carries part of the human BK papovavirus with 0.5 kilobases of (2'-5')oligoadenylate (2-5A) synthetase cDNA inserted in inverted orientation downstream from the virion proteins (VP) promoter and the neomycin-resistance gene neo under the control of a simian virus 40 promoter. Cells transfected with this vector and selected for resistance to the neomycin derivative G418 synthesized RNA complementary to 2-5A synthetase mRNA. These cells lacked 2-5A synthetase activity, and the enzyme was not inducible by interferon. In contrast, 2-5A synthetase was induced in cells transfected with a control vector without the cDNA insert. Such cells were protected by interferon from RNA viruses, whereas cells lacking 2-5A synthetase were not protected from encephalomyocarditis virus, vesicular stomatitis virus, and Sindbis virus but were fully protected from influenza virus. These findings show that a high level of 2-5A synthetase is required for interferon-induced protection from the cytoplasmic RNA viruses tested.</jats:p> Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. Proceedings of the National Academy of Sciences |
| spellingShingle | De Benedetti, A, Pytel, B A, Baglioni, C, Proceedings of the National Academy of Sciences, Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections., Multidisciplinary |
| title | Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_full | Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_fullStr | Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_full_unstemmed | Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_short | Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| title_sort | loss of (2'-5')oligoadenylate synthetase activity by production of antisense rna results in lack of protection by interferon from viral infections. |
| title_unstemmed | Loss of (2'-5')oligoadenylate synthetase activity by production of antisense RNA results in lack of protection by interferon from viral infections. |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.84.3.658 |