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Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | Proceedings of the National Academy of Sciences, 70, 1973, 1, S. 46-49 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Di Mayorca, Giampiero Greenblatt, Melvin Trauthen, Thomas Soller, Arthur Giordano, Roberto Di Mayorca, Giampiero Greenblatt, Melvin Trauthen, Thomas Soller, Arthur Giordano, Roberto |
|---|---|
| author |
Di Mayorca, Giampiero Greenblatt, Melvin Trauthen, Thomas Soller, Arthur Giordano, Roberto |
| spellingShingle |
Di Mayorca, Giampiero Greenblatt, Melvin Trauthen, Thomas Soller, Arthur Giordano, Roberto Proceedings of the National Academy of Sciences Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State Multidisciplinary |
| author_sort |
di mayorca, giampiero |
| spelling |
Di Mayorca, Giampiero Greenblatt, Melvin Trauthen, Thomas Soller, Arthur Giordano, Roberto 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.70.1.46 <jats:p> All the BHK <jats:sub>21</jats:sub> Clone 13 cells transformed by dimethylnitrosamine or nitrosomethylurea, and a single “spontaneously” transformed clone obtained in our laboratory, exhibit a conditional state of the transformed phenotype. Upon growth at 38.5°, they have a transformed phenotype (clonal morphology and the ability to plate in soft agar); when grown at 32°, their phenotype is normal. The conditional state of these cells, however, does not extend to their growth characteristics; their plating efficiency at the two temperatures in liquid medium is similar. Conversion from the normal to the transformed phenotype and the reverse is possible by temperature shifts. A working hypothesis is proposed suggesting that this phenomenon may be caused by a temperature-sensitive mutation of a cellular gene whose product is responsible for maintaining the normal phenotype. The high temperature would then affect either the synthesis or the function of the protein carrying the temperature-sensitive alteration and, as a consequence, the transformed phenotype would be expressed. </jats:p> Malignant Transformation of BHK <sub>21</sub> Clone 13 Cells <i>In Vitro</i> by Nitrosamines—A Conditional State Proceedings of the National Academy of Sciences |
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| title |
Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_unstemmed |
Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_full |
Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_fullStr |
Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_full_unstemmed |
Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_short |
Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_sort |
malignant transformation of bhk
<sub>21</sub>
clone 13 cells
<i>in vitro</i>
by nitrosamines—a conditional state |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.70.1.46 |
| publishDate |
1973 |
| physical |
46-49 |
| description |
<jats:p>
All the BHK
<jats:sub>21</jats:sub>
Clone 13 cells transformed by dimethylnitrosamine or nitrosomethylurea, and a single “spontaneously” transformed clone obtained in our laboratory, exhibit a conditional state of the transformed phenotype. Upon growth at 38.5°, they have a transformed phenotype (clonal morphology and the ability to plate in soft agar); when grown at 32°, their phenotype is normal. The conditional state of these cells, however, does not extend to their growth characteristics; their plating efficiency at the two temperatures in liquid medium is similar. Conversion from the normal to the transformed phenotype and the reverse is possible by temperature shifts. A working hypothesis is proposed suggesting that this phenomenon may be caused by a temperature-sensitive mutation of a cellular gene whose product is responsible for maintaining the normal phenotype. The high temperature would then affect either the synthesis or the function of the protein carrying the temperature-sensitive alteration and, as a consequence, the transformed phenotype would be expressed.
</jats:p> |
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| author | Di Mayorca, Giampiero, Greenblatt, Melvin, Trauthen, Thomas, Soller, Arthur, Giordano, Roberto |
| author_facet | Di Mayorca, Giampiero, Greenblatt, Melvin, Trauthen, Thomas, Soller, Arthur, Giordano, Roberto, Di Mayorca, Giampiero, Greenblatt, Melvin, Trauthen, Thomas, Soller, Arthur, Giordano, Roberto |
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| description | <jats:p> All the BHK <jats:sub>21</jats:sub> Clone 13 cells transformed by dimethylnitrosamine or nitrosomethylurea, and a single “spontaneously” transformed clone obtained in our laboratory, exhibit a conditional state of the transformed phenotype. Upon growth at 38.5°, they have a transformed phenotype (clonal morphology and the ability to plate in soft agar); when grown at 32°, their phenotype is normal. The conditional state of these cells, however, does not extend to their growth characteristics; their plating efficiency at the two temperatures in liquid medium is similar. Conversion from the normal to the transformed phenotype and the reverse is possible by temperature shifts. A working hypothesis is proposed suggesting that this phenomenon may be caused by a temperature-sensitive mutation of a cellular gene whose product is responsible for maintaining the normal phenotype. The high temperature would then affect either the synthesis or the function of the protein carrying the temperature-sensitive alteration and, as a consequence, the transformed phenotype would be expressed. </jats:p> |
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| imprint | Proceedings of the National Academy of Sciences, 1973 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 1973 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| physical | 46-49 |
| publishDate | 1973 |
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| spelling | Di Mayorca, Giampiero Greenblatt, Melvin Trauthen, Thomas Soller, Arthur Giordano, Roberto 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.70.1.46 <jats:p> All the BHK <jats:sub>21</jats:sub> Clone 13 cells transformed by dimethylnitrosamine or nitrosomethylurea, and a single “spontaneously” transformed clone obtained in our laboratory, exhibit a conditional state of the transformed phenotype. Upon growth at 38.5°, they have a transformed phenotype (clonal morphology and the ability to plate in soft agar); when grown at 32°, their phenotype is normal. The conditional state of these cells, however, does not extend to their growth characteristics; their plating efficiency at the two temperatures in liquid medium is similar. Conversion from the normal to the transformed phenotype and the reverse is possible by temperature shifts. A working hypothesis is proposed suggesting that this phenomenon may be caused by a temperature-sensitive mutation of a cellular gene whose product is responsible for maintaining the normal phenotype. The high temperature would then affect either the synthesis or the function of the protein carrying the temperature-sensitive alteration and, as a consequence, the transformed phenotype would be expressed. </jats:p> Malignant Transformation of BHK <sub>21</sub> Clone 13 Cells <i>In Vitro</i> by Nitrosamines—A Conditional State Proceedings of the National Academy of Sciences |
| spellingShingle | Di Mayorca, Giampiero, Greenblatt, Melvin, Trauthen, Thomas, Soller, Arthur, Giordano, Roberto, Proceedings of the National Academy of Sciences, Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State, Multidisciplinary |
| title | Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_full | Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_fullStr | Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_full_unstemmed | Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_short | Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| title_sort | malignant transformation of bhk <sub>21</sub> clone 13 cells <i>in vitro</i> by nitrosamines—a conditional state |
| title_unstemmed | Malignant Transformation of BHK 21 Clone 13 Cells In Vitro by Nitrosamines—A Conditional State |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.70.1.46 |