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Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Proceedings of the National Academy of Sciences, 111, 2014, 2, S. 681-686 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Zhao, Ziqing W. Roy, Rahul Gebhardt, J. Christof M. Suter, David M. Chapman, Alec R. Xie, X. Sunney Zhao, Ziqing W. Roy, Rahul Gebhardt, J. Christof M. Suter, David M. Chapman, Alec R. Xie, X. Sunney |
|---|---|
| author |
Zhao, Ziqing W. Roy, Rahul Gebhardt, J. Christof M. Suter, David M. Chapman, Alec R. Xie, X. Sunney |
| spellingShingle |
Zhao, Ziqing W. Roy, Rahul Gebhardt, J. Christof M. Suter, David M. Chapman, Alec R. Xie, X. Sunney Proceedings of the National Academy of Sciences Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy Multidisciplinary |
| author_sort |
zhao, ziqing w. |
| spelling |
Zhao, Ziqing W. Roy, Rahul Gebhardt, J. Christof M. Suter, David M. Chapman, Alec R. Xie, X. Sunney 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1318496111 <jats:title>Significance</jats:title> <jats:p>We developed an optical imaging technique that combines reflected light-sheet illumination with superresolution microscopy, allowing us to image inside mammalian nuclei at subdiffraction-limit resolution and to count biomolecules with single-copy accuracy. Applying this technique to probe the spatial organization of RNA polymerase II-mediated transcription, we found that the majority of the transcription foci consist of only one RNAP II molecule, contrary to previous proposals. By quantifying the global extent of clustering across RNAP II molecules in the nucleus, we provide clear and convincing answers to the controversy surrounding the prevalent existence of “transcription factories.” Moreover, our work presents imaging and analysis tools for the quantitative characterization of nuclear structures, which could be generally applied to probe many other mammalian systems.</jats:p> Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy Proceedings of the National Academy of Sciences |
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| title |
Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_unstemmed |
Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_full |
Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_fullStr |
Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_full_unstemmed |
Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_short |
Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_sort |
spatial organization of rna polymerase ii inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
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Multidisciplinary |
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http://dx.doi.org/10.1073/pnas.1318496111 |
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2014 |
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681-686 |
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<jats:title>Significance</jats:title>
<jats:p>We developed an optical imaging technique that combines reflected light-sheet illumination with superresolution microscopy, allowing us to image inside mammalian nuclei at subdiffraction-limit resolution and to count biomolecules with single-copy accuracy. Applying this technique to probe the spatial organization of RNA polymerase II-mediated transcription, we found that the majority of the transcription foci consist of only one RNAP II molecule, contrary to previous proposals. By quantifying the global extent of clustering across RNAP II molecules in the nucleus, we provide clear and convincing answers to the controversy surrounding the prevalent existence of “transcription factories.” Moreover, our work presents imaging and analysis tools for the quantitative characterization of nuclear structures, which could be generally applied to probe many other mammalian systems.</jats:p> |
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| author | Zhao, Ziqing W., Roy, Rahul, Gebhardt, J. Christof M., Suter, David M., Chapman, Alec R., Xie, X. Sunney |
| author_facet | Zhao, Ziqing W., Roy, Rahul, Gebhardt, J. Christof M., Suter, David M., Chapman, Alec R., Xie, X. Sunney, Zhao, Ziqing W., Roy, Rahul, Gebhardt, J. Christof M., Suter, David M., Chapman, Alec R., Xie, X. Sunney |
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| description | <jats:title>Significance</jats:title> <jats:p>We developed an optical imaging technique that combines reflected light-sheet illumination with superresolution microscopy, allowing us to image inside mammalian nuclei at subdiffraction-limit resolution and to count biomolecules with single-copy accuracy. Applying this technique to probe the spatial organization of RNA polymerase II-mediated transcription, we found that the majority of the transcription foci consist of only one RNAP II molecule, contrary to previous proposals. By quantifying the global extent of clustering across RNAP II molecules in the nucleus, we provide clear and convincing answers to the controversy surrounding the prevalent existence of “transcription factories.” Moreover, our work presents imaging and analysis tools for the quantitative characterization of nuclear structures, which could be generally applied to probe many other mammalian systems.</jats:p> |
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| spelling | Zhao, Ziqing W. Roy, Rahul Gebhardt, J. Christof M. Suter, David M. Chapman, Alec R. Xie, X. Sunney 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1318496111 <jats:title>Significance</jats:title> <jats:p>We developed an optical imaging technique that combines reflected light-sheet illumination with superresolution microscopy, allowing us to image inside mammalian nuclei at subdiffraction-limit resolution and to count biomolecules with single-copy accuracy. Applying this technique to probe the spatial organization of RNA polymerase II-mediated transcription, we found that the majority of the transcription foci consist of only one RNAP II molecule, contrary to previous proposals. By quantifying the global extent of clustering across RNAP II molecules in the nucleus, we provide clear and convincing answers to the controversy surrounding the prevalent existence of “transcription factories.” Moreover, our work presents imaging and analysis tools for the quantitative characterization of nuclear structures, which could be generally applied to probe many other mammalian systems.</jats:p> Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy Proceedings of the National Academy of Sciences |
| spellingShingle | Zhao, Ziqing W., Roy, Rahul, Gebhardt, J. Christof M., Suter, David M., Chapman, Alec R., Xie, X. Sunney, Proceedings of the National Academy of Sciences, Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy, Multidisciplinary |
| title | Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_full | Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_fullStr | Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_full_unstemmed | Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_short | Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_sort | spatial organization of rna polymerase ii inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| title_unstemmed | Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.1318496111 |