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Identification and mechanism of regulation of the zebrafish dorsal determinant
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 108, 2011, 38, S. 15876-15880 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Lu, Fu-I Thisse, Christine Thisse, Bernard Lu, Fu-I Thisse, Christine Thisse, Bernard |
|---|---|
| author |
Lu, Fu-I Thisse, Christine Thisse, Bernard |
| spellingShingle |
Lu, Fu-I Thisse, Christine Thisse, Bernard Proceedings of the National Academy of Sciences Identification and mechanism of regulation of the zebrafish dorsal determinant Multidisciplinary |
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lu, fu-i |
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Lu, Fu-I Thisse, Christine Thisse, Bernard 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1106801108 <jats:p> In vertebrates, the animal–vegetal axis is determined during oogenesis and at ovulation, the egg is radially symmetric. In anamniotes, following fertilization, a microtubule-dependent movement leads to the displacement of maternal dorsal determinants from the vegetal pole to the future dorsal side of the embryo, providing the initial breaking of radial symmetry [Weaver C, Kimelman D (2004) <jats:italic>Development</jats:italic> 131:3491–3499]. These dorsal determinants induce β-catenin nuclear translocation in dorsal cells of the blastula. Previous work in amphibians has shown that secreted Wnt11/5a complexes, regulated by the Wnt antagonist Dkk-1, are required for the initiation of embryonic axis formation [Cha et al. (2009) <jats:italic>Curr Biol</jats:italic> 29:1573–1580]. In the current study, we determined that the vegetal maternal dorsal determinant in fish is not the Wnt11/5a complex but the canonical Wnt, Wnt8a. Translation of this mRNA and secretion of the Wnt8a protein result in a dorsal-to-ventral gradient of Wnt stimulation, extending across the entire embryo. This gradient is counterbalanced by two Wnt inhibitors, Sfrp1a and Frzb. These proteins are essential to restrict the activation of the canonical Wnt pathway to the dorsal marginal blastomeres by defining the domain where the Wnt8a activity gradient is above the threshold value necessary for triggering the canonical β-catenin pathway. In summary, this study establishes that the zebrafish maternal dorsal determinant, Wnt8a, is required to localize the primary dorsal center, and that the extent of this domain is defined by the activity of two maternally provided Wnt antagonists, Sfrp1a and Frzb. </jats:p> Identification and mechanism of regulation of the zebrafish dorsal determinant Proceedings of the National Academy of Sciences |
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Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_unstemmed |
Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_full |
Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_fullStr |
Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_full_unstemmed |
Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_short |
Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_sort |
identification and mechanism of regulation of the zebrafish dorsal determinant |
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Multidisciplinary |
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http://dx.doi.org/10.1073/pnas.1106801108 |
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2011 |
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15876-15880 |
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<jats:p>
In vertebrates, the animal–vegetal axis is determined during oogenesis and at ovulation, the egg is radially symmetric. In anamniotes, following fertilization, a microtubule-dependent movement leads to the displacement of maternal dorsal determinants from the vegetal pole to the future dorsal side of the embryo, providing the initial breaking of radial symmetry [Weaver C, Kimelman D (2004)
<jats:italic>Development</jats:italic>
131:3491–3499]. These dorsal determinants induce β-catenin nuclear translocation in dorsal cells of the blastula. Previous work in amphibians has shown that secreted Wnt11/5a complexes, regulated by the Wnt antagonist Dkk-1, are required for the initiation of embryonic axis formation [Cha et al. (2009)
<jats:italic>Curr Biol</jats:italic>
29:1573–1580]. In the current study, we determined that the vegetal maternal dorsal determinant in fish is not the Wnt11/5a complex but the canonical Wnt, Wnt8a. Translation of this mRNA and secretion of the Wnt8a protein result in a dorsal-to-ventral gradient of Wnt stimulation, extending across the entire embryo. This gradient is counterbalanced by two Wnt inhibitors, Sfrp1a and Frzb. These proteins are essential to restrict the activation of the canonical Wnt pathway to the dorsal marginal blastomeres by defining the domain where the Wnt8a activity gradient is above the threshold value necessary for triggering the canonical β-catenin pathway. In summary, this study establishes that the zebrafish maternal dorsal determinant, Wnt8a, is required to localize the primary dorsal center, and that the extent of this domain is defined by the activity of two maternally provided Wnt antagonists, Sfrp1a and Frzb.
</jats:p> |
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| description | <jats:p> In vertebrates, the animal–vegetal axis is determined during oogenesis and at ovulation, the egg is radially symmetric. In anamniotes, following fertilization, a microtubule-dependent movement leads to the displacement of maternal dorsal determinants from the vegetal pole to the future dorsal side of the embryo, providing the initial breaking of radial symmetry [Weaver C, Kimelman D (2004) <jats:italic>Development</jats:italic> 131:3491–3499]. These dorsal determinants induce β-catenin nuclear translocation in dorsal cells of the blastula. Previous work in amphibians has shown that secreted Wnt11/5a complexes, regulated by the Wnt antagonist Dkk-1, are required for the initiation of embryonic axis formation [Cha et al. (2009) <jats:italic>Curr Biol</jats:italic> 29:1573–1580]. In the current study, we determined that the vegetal maternal dorsal determinant in fish is not the Wnt11/5a complex but the canonical Wnt, Wnt8a. Translation of this mRNA and secretion of the Wnt8a protein result in a dorsal-to-ventral gradient of Wnt stimulation, extending across the entire embryo. This gradient is counterbalanced by two Wnt inhibitors, Sfrp1a and Frzb. These proteins are essential to restrict the activation of the canonical Wnt pathway to the dorsal marginal blastomeres by defining the domain where the Wnt8a activity gradient is above the threshold value necessary for triggering the canonical β-catenin pathway. In summary, this study establishes that the zebrafish maternal dorsal determinant, Wnt8a, is required to localize the primary dorsal center, and that the extent of this domain is defined by the activity of two maternally provided Wnt antagonists, Sfrp1a and Frzb. </jats:p> |
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| spelling | Lu, Fu-I Thisse, Christine Thisse, Bernard 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1106801108 <jats:p> In vertebrates, the animal–vegetal axis is determined during oogenesis and at ovulation, the egg is radially symmetric. In anamniotes, following fertilization, a microtubule-dependent movement leads to the displacement of maternal dorsal determinants from the vegetal pole to the future dorsal side of the embryo, providing the initial breaking of radial symmetry [Weaver C, Kimelman D (2004) <jats:italic>Development</jats:italic> 131:3491–3499]. These dorsal determinants induce β-catenin nuclear translocation in dorsal cells of the blastula. Previous work in amphibians has shown that secreted Wnt11/5a complexes, regulated by the Wnt antagonist Dkk-1, are required for the initiation of embryonic axis formation [Cha et al. (2009) <jats:italic>Curr Biol</jats:italic> 29:1573–1580]. In the current study, we determined that the vegetal maternal dorsal determinant in fish is not the Wnt11/5a complex but the canonical Wnt, Wnt8a. Translation of this mRNA and secretion of the Wnt8a protein result in a dorsal-to-ventral gradient of Wnt stimulation, extending across the entire embryo. This gradient is counterbalanced by two Wnt inhibitors, Sfrp1a and Frzb. These proteins are essential to restrict the activation of the canonical Wnt pathway to the dorsal marginal blastomeres by defining the domain where the Wnt8a activity gradient is above the threshold value necessary for triggering the canonical β-catenin pathway. In summary, this study establishes that the zebrafish maternal dorsal determinant, Wnt8a, is required to localize the primary dorsal center, and that the extent of this domain is defined by the activity of two maternally provided Wnt antagonists, Sfrp1a and Frzb. </jats:p> Identification and mechanism of regulation of the zebrafish dorsal determinant Proceedings of the National Academy of Sciences |
| spellingShingle | Lu, Fu-I, Thisse, Christine, Thisse, Bernard, Proceedings of the National Academy of Sciences, Identification and mechanism of regulation of the zebrafish dorsal determinant, Multidisciplinary |
| title | Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_full | Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_fullStr | Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_full_unstemmed | Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_short | Identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_sort | identification and mechanism of regulation of the zebrafish dorsal determinant |
| title_unstemmed | Identification and mechanism of regulation of the zebrafish dorsal determinant |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.1106801108 |