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Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water

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Zeitschriftentitel: Proceedings of the National Academy of Sciences
Personen und Körperschaften: Zeng, Hongbo, Hwang, Dong Soo, Israelachvili, Jacob N., Waite, J. Herbert
In: Proceedings of the National Academy of Sciences, 107, 2010, 29, S. 12850-12853
Format: E-Article
Sprache: Englisch
veröffentlicht:
Proceedings of the National Academy of Sciences
Schlagwörter:
Multidisciplinary
author_facet Zeng, Hongbo
Hwang, Dong Soo
Israelachvili, Jacob N.
Waite, J. Herbert
Zeng, Hongbo
Hwang, Dong Soo
Israelachvili, Jacob N.
Waite, J. Herbert
author Zeng, Hongbo
Hwang, Dong Soo
Israelachvili, Jacob N.
Waite, J. Herbert
spellingShingle Zeng, Hongbo
Hwang, Dong Soo
Israelachvili, Jacob N.
Waite, J. Herbert
Proceedings of the National Academy of Sciences
Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
Multidisciplinary
author_sort zeng, hongbo
spelling Zeng, Hongbo Hwang, Dong Soo Israelachvili, Jacob N. Waite, J. Herbert 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1007416107 <jats:p> Metal-containing polymer networks are widespread in biology, particularly for load-bearing exoskeletal biomaterials. <jats:italic>Mytilus</jats:italic> byssal cuticle is an especially interesting case containing moderate levels of Fe <jats:sup>3+</jats:sup> and cuticle protein—mussel foot protein-1 (mfp-1), which has a peculiar combination of high hardness and high extensibility. Mfp-1, containing 13 mol % of dopa (3, 4-dihydroxyphenylalanine) side-chains, is highly positively charged polyelectrolyte (pI ∼ 10) and didn’t show any cohesive tendencies in previous surface forces apparatus (SFA) studies. Here, we show that Fe <jats:sup>3+</jats:sup> ions can mediate unusually strong interactions between the positively charged proteins. Using an SFA, Fe <jats:sup>3+</jats:sup> was observed to impart robust bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub> ≈ 4.3 mJ/m <jats:sup>2</jats:sup> ) between two noninteracting mfp-1 films in aqueous buffer approaching the ionic strength of seawater. The Fe <jats:sup>3+</jats:sup> bridging between the mfp-1-coated surfaces is fully reversible in water, increasing with contact time and iron concentration up to 10 μM; at 100 μM, Fe <jats:sup>3+</jats:sup> bridging adhesion is abolished. Bridging is apparently due to the formation of multivalent dopa-iron complexes. Similar Fe-mediated bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub> ≈ 5.7 mJ/m <jats:sup>2</jats:sup> ) by a smaller recombinant dopa-containing analogue indicates that bridging is largely independent of molecular weight and posttranslational modifications other than dopa. The results suggest that dopa-metal interactions may provide an energetic new paradigm for engineering strong, self-healing interactions between polymers under water. </jats:p> Strong reversible Fe <sup>3+</sup> -mediated bridging between dopa-containing protein films in water Proceedings of the National Academy of Sciences
doi_str_mv 10.1073/pnas.1007416107
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title Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_unstemmed Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_full Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_fullStr Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_full_unstemmed Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_short Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_sort strong reversible fe <sup>3+</sup> -mediated bridging between dopa-containing protein films in water
topic Multidisciplinary
url http://dx.doi.org/10.1073/pnas.1007416107
publishDate 2010
physical 12850-12853
description <jats:p> Metal-containing polymer networks are widespread in biology, particularly for load-bearing exoskeletal biomaterials. <jats:italic>Mytilus</jats:italic> byssal cuticle is an especially interesting case containing moderate levels of Fe <jats:sup>3+</jats:sup> and cuticle protein—mussel foot protein-1 (mfp-1), which has a peculiar combination of high hardness and high extensibility. Mfp-1, containing 13 mol % of dopa (3, 4-dihydroxyphenylalanine) side-chains, is highly positively charged polyelectrolyte (pI ∼ 10) and didn’t show any cohesive tendencies in previous surface forces apparatus (SFA) studies. Here, we show that Fe <jats:sup>3+</jats:sup> ions can mediate unusually strong interactions between the positively charged proteins. Using an SFA, Fe <jats:sup>3+</jats:sup> was observed to impart robust bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub>  ≈ 4.3 mJ/m <jats:sup>2</jats:sup> ) between two noninteracting mfp-1 films in aqueous buffer approaching the ionic strength of seawater. The Fe <jats:sup>3+</jats:sup> bridging between the mfp-1-coated surfaces is fully reversible in water, increasing with contact time and iron concentration up to 10 μM; at 100 μM, Fe <jats:sup>3+</jats:sup> bridging adhesion is abolished. Bridging is apparently due to the formation of multivalent dopa-iron complexes. Similar Fe-mediated bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub>  ≈ 5.7 mJ/m <jats:sup>2</jats:sup> ) by a smaller recombinant dopa-containing analogue indicates that bridging is largely independent of molecular weight and posttranslational modifications other than dopa. The results suggest that dopa-metal interactions may provide an energetic new paradigm for engineering strong, self-healing interactions between polymers under water. </jats:p>
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author Zeng, Hongbo, Hwang, Dong Soo, Israelachvili, Jacob N., Waite, J. Herbert
author_facet Zeng, Hongbo, Hwang, Dong Soo, Israelachvili, Jacob N., Waite, J. Herbert, Zeng, Hongbo, Hwang, Dong Soo, Israelachvili, Jacob N., Waite, J. Herbert
author_sort zeng, hongbo
container_issue 29
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container_title Proceedings of the National Academy of Sciences
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description <jats:p> Metal-containing polymer networks are widespread in biology, particularly for load-bearing exoskeletal biomaterials. <jats:italic>Mytilus</jats:italic> byssal cuticle is an especially interesting case containing moderate levels of Fe <jats:sup>3+</jats:sup> and cuticle protein—mussel foot protein-1 (mfp-1), which has a peculiar combination of high hardness and high extensibility. Mfp-1, containing 13 mol % of dopa (3, 4-dihydroxyphenylalanine) side-chains, is highly positively charged polyelectrolyte (pI ∼ 10) and didn’t show any cohesive tendencies in previous surface forces apparatus (SFA) studies. Here, we show that Fe <jats:sup>3+</jats:sup> ions can mediate unusually strong interactions between the positively charged proteins. Using an SFA, Fe <jats:sup>3+</jats:sup> was observed to impart robust bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub>  ≈ 4.3 mJ/m <jats:sup>2</jats:sup> ) between two noninteracting mfp-1 films in aqueous buffer approaching the ionic strength of seawater. The Fe <jats:sup>3+</jats:sup> bridging between the mfp-1-coated surfaces is fully reversible in water, increasing with contact time and iron concentration up to 10 μM; at 100 μM, Fe <jats:sup>3+</jats:sup> bridging adhesion is abolished. Bridging is apparently due to the formation of multivalent dopa-iron complexes. Similar Fe-mediated bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub>  ≈ 5.7 mJ/m <jats:sup>2</jats:sup> ) by a smaller recombinant dopa-containing analogue indicates that bridging is largely independent of molecular weight and posttranslational modifications other than dopa. The results suggest that dopa-metal interactions may provide an energetic new paradigm for engineering strong, self-healing interactions between polymers under water. </jats:p>
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spelling Zeng, Hongbo Hwang, Dong Soo Israelachvili, Jacob N. Waite, J. Herbert 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1007416107 <jats:p> Metal-containing polymer networks are widespread in biology, particularly for load-bearing exoskeletal biomaterials. <jats:italic>Mytilus</jats:italic> byssal cuticle is an especially interesting case containing moderate levels of Fe <jats:sup>3+</jats:sup> and cuticle protein—mussel foot protein-1 (mfp-1), which has a peculiar combination of high hardness and high extensibility. Mfp-1, containing 13 mol % of dopa (3, 4-dihydroxyphenylalanine) side-chains, is highly positively charged polyelectrolyte (pI ∼ 10) and didn’t show any cohesive tendencies in previous surface forces apparatus (SFA) studies. Here, we show that Fe <jats:sup>3+</jats:sup> ions can mediate unusually strong interactions between the positively charged proteins. Using an SFA, Fe <jats:sup>3+</jats:sup> was observed to impart robust bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub> ≈ 4.3 mJ/m <jats:sup>2</jats:sup> ) between two noninteracting mfp-1 films in aqueous buffer approaching the ionic strength of seawater. The Fe <jats:sup>3+</jats:sup> bridging between the mfp-1-coated surfaces is fully reversible in water, increasing with contact time and iron concentration up to 10 μM; at 100 μM, Fe <jats:sup>3+</jats:sup> bridging adhesion is abolished. Bridging is apparently due to the formation of multivalent dopa-iron complexes. Similar Fe-mediated bridging ( <jats:italic>W</jats:italic> <jats:sub>ad</jats:sub> ≈ 5.7 mJ/m <jats:sup>2</jats:sup> ) by a smaller recombinant dopa-containing analogue indicates that bridging is largely independent of molecular weight and posttranslational modifications other than dopa. The results suggest that dopa-metal interactions may provide an energetic new paradigm for engineering strong, self-healing interactions between polymers under water. </jats:p> Strong reversible Fe <sup>3+</sup> -mediated bridging between dopa-containing protein films in water Proceedings of the National Academy of Sciences
spellingShingle Zeng, Hongbo, Hwang, Dong Soo, Israelachvili, Jacob N., Waite, J. Herbert, Proceedings of the National Academy of Sciences, Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water, Multidisciplinary
title Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_full Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_fullStr Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_full_unstemmed Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_short Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
title_sort strong reversible fe <sup>3+</sup> -mediated bridging between dopa-containing protein films in water
title_unstemmed Strong reversible Fe 3+ -mediated bridging between dopa-containing protein films in water
topic Multidisciplinary
url http://dx.doi.org/10.1073/pnas.1007416107