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Illuminating structural proteins in viral “dark matter” with metaproteomics
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Proceedings of the National Academy of Sciences, 113, 2016, 9, S. 2436-2441 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Brum, Jennifer R. Ignacio-Espinoza, J. Cesar Kim, Eun-Hae Trubl, Gareth Jones, Robert M. Roux, Simon VerBerkmoes, Nathan C. Rich, Virginia I. Sullivan, Matthew B. Brum, Jennifer R. Ignacio-Espinoza, J. Cesar Kim, Eun-Hae Trubl, Gareth Jones, Robert M. Roux, Simon VerBerkmoes, Nathan C. Rich, Virginia I. Sullivan, Matthew B. |
|---|---|
| author |
Brum, Jennifer R. Ignacio-Espinoza, J. Cesar Kim, Eun-Hae Trubl, Gareth Jones, Robert M. Roux, Simon VerBerkmoes, Nathan C. Rich, Virginia I. Sullivan, Matthew B. |
| spellingShingle |
Brum, Jennifer R. Ignacio-Espinoza, J. Cesar Kim, Eun-Hae Trubl, Gareth Jones, Robert M. Roux, Simon VerBerkmoes, Nathan C. Rich, Virginia I. Sullivan, Matthew B. Proceedings of the National Academy of Sciences Illuminating structural proteins in viral “dark matter” with metaproteomics Multidisciplinary |
| author_sort |
brum, jennifer r. |
| spelling |
Brum, Jennifer R. Ignacio-Espinoza, J. Cesar Kim, Eun-Hae Trubl, Gareth Jones, Robert M. Roux, Simon VerBerkmoes, Nathan C. Rich, Virginia I. Sullivan, Matthew B. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1525139113 <jats:title>Significance</jats:title> <jats:p>Marine viruses are abundant and have substantial ecosystem impacts, yet their study is hampered by the dominance of unannotated viral genes. Here, we use metaproteomics and metagenomics to examine virion-associated proteins in marine viral communities, providing tentative functions for 677,000 viral genomic sequences and the majority of previously unknown virion-associated proteins in these samples. The five most abundant protein groups comprised 67% of the metaproteomes and were tentatively identified as capsid proteins of predominantly unknown viruses, all of which putatively contain a protein fold that may be the most abundant biological structure on Earth. This methodological approach is thus shown to be a powerful way to increase our knowledge of the most numerous biological entities on the planet.</jats:p> Illuminating structural proteins in viral “dark matter” with metaproteomics Proceedings of the National Academy of Sciences |
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Illuminating structural proteins in viral “dark matter” with metaproteomics |
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Illuminating structural proteins in viral “dark matter” with metaproteomics |
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Illuminating structural proteins in viral “dark matter” with metaproteomics |
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Illuminating structural proteins in viral “dark matter” with metaproteomics |
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Illuminating structural proteins in viral “dark matter” with metaproteomics |
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illuminating structural proteins in viral “dark matter” with metaproteomics |
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Multidisciplinary |
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2016 |
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<jats:title>Significance</jats:title>
<jats:p>Marine viruses are abundant and have substantial ecosystem impacts, yet their study is hampered by the dominance of unannotated viral genes. Here, we use metaproteomics and metagenomics to examine virion-associated proteins in marine viral communities, providing tentative functions for 677,000 viral genomic sequences and the majority of previously unknown virion-associated proteins in these samples. The five most abundant protein groups comprised 67% of the metaproteomes and were tentatively identified as capsid proteins of predominantly unknown viruses, all of which putatively contain a protein fold that may be the most abundant biological structure on Earth. This methodological approach is thus shown to be a powerful way to increase our knowledge of the most numerous biological entities on the planet.</jats:p> |
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| author | Brum, Jennifer R., Ignacio-Espinoza, J. Cesar, Kim, Eun-Hae, Trubl, Gareth, Jones, Robert M., Roux, Simon, VerBerkmoes, Nathan C., Rich, Virginia I., Sullivan, Matthew B. |
| author_facet | Brum, Jennifer R., Ignacio-Espinoza, J. Cesar, Kim, Eun-Hae, Trubl, Gareth, Jones, Robert M., Roux, Simon, VerBerkmoes, Nathan C., Rich, Virginia I., Sullivan, Matthew B., Brum, Jennifer R., Ignacio-Espinoza, J. Cesar, Kim, Eun-Hae, Trubl, Gareth, Jones, Robert M., Roux, Simon, VerBerkmoes, Nathan C., Rich, Virginia I., Sullivan, Matthew B. |
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| description | <jats:title>Significance</jats:title> <jats:p>Marine viruses are abundant and have substantial ecosystem impacts, yet their study is hampered by the dominance of unannotated viral genes. Here, we use metaproteomics and metagenomics to examine virion-associated proteins in marine viral communities, providing tentative functions for 677,000 viral genomic sequences and the majority of previously unknown virion-associated proteins in these samples. The five most abundant protein groups comprised 67% of the metaproteomes and were tentatively identified as capsid proteins of predominantly unknown viruses, all of which putatively contain a protein fold that may be the most abundant biological structure on Earth. This methodological approach is thus shown to be a powerful way to increase our knowledge of the most numerous biological entities on the planet.</jats:p> |
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| spelling | Brum, Jennifer R. Ignacio-Espinoza, J. Cesar Kim, Eun-Hae Trubl, Gareth Jones, Robert M. Roux, Simon VerBerkmoes, Nathan C. Rich, Virginia I. Sullivan, Matthew B. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.1525139113 <jats:title>Significance</jats:title> <jats:p>Marine viruses are abundant and have substantial ecosystem impacts, yet their study is hampered by the dominance of unannotated viral genes. Here, we use metaproteomics and metagenomics to examine virion-associated proteins in marine viral communities, providing tentative functions for 677,000 viral genomic sequences and the majority of previously unknown virion-associated proteins in these samples. The five most abundant protein groups comprised 67% of the metaproteomes and were tentatively identified as capsid proteins of predominantly unknown viruses, all of which putatively contain a protein fold that may be the most abundant biological structure on Earth. This methodological approach is thus shown to be a powerful way to increase our knowledge of the most numerous biological entities on the planet.</jats:p> Illuminating structural proteins in viral “dark matter” with metaproteomics Proceedings of the National Academy of Sciences |
| spellingShingle | Brum, Jennifer R., Ignacio-Espinoza, J. Cesar, Kim, Eun-Hae, Trubl, Gareth, Jones, Robert M., Roux, Simon, VerBerkmoes, Nathan C., Rich, Virginia I., Sullivan, Matthew B., Proceedings of the National Academy of Sciences, Illuminating structural proteins in viral “dark matter” with metaproteomics, Multidisciplinary |
| title | Illuminating structural proteins in viral “dark matter” with metaproteomics |
| title_full | Illuminating structural proteins in viral “dark matter” with metaproteomics |
| title_fullStr | Illuminating structural proteins in viral “dark matter” with metaproteomics |
| title_full_unstemmed | Illuminating structural proteins in viral “dark matter” with metaproteomics |
| title_short | Illuminating structural proteins in viral “dark matter” with metaproteomics |
| title_sort | illuminating structural proteins in viral “dark matter” with metaproteomics |
| title_unstemmed | Illuminating structural proteins in viral “dark matter” with metaproteomics |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.1525139113 |