Recessive cardiac phenotypes in induced pluripotent stem cell models of Jervell and Lange-Nielsen syndrome: Disease mechanisms and pharmacological rescue

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Bibliographische Detailangaben
Zeitschriftentitel:	Proceedings of the National Academy of Sciences
Personen und Körperschaften:	Zhang, Miao, D’Aniello, Cristina, Verkerk, Arie O., Wrobel, Eva, Frank, Stefan, Ward-van Oostwaard, Dorien, Piccini, Ilaria, Freund, Christian, Rao, Jyoti, Seebohm, Guiscard, Atsma, Douwe E., Schulze-Bahr, Eric, Mummery, Christine L., Greber, Boris, Bellin, Milena
In:	Proceedings of the National Academy of Sciences, 111, 2014, 50
Format:	E-Article
Sprache:	Englisch
veröffentlicht:	Proceedings of the National Academy of Sciences
Schlagwörter:	Multidisciplinary

Details
Zusammenfassung:	<jats:title>Significance</jats:title> <jats:p> There are few laboratory models that recapitulate human cardiac disease. Here, we created human cell models for Jervell and Lange-Nielsen syndrome (JLNS) in vitro, based on human induced pluripotent stem cells (hiPSCs). JLNS is one of the most severe disorders of heart rhythm and can cause sudden death in young patients. JLNS is inherited recessively and is caused by homozygous mutations in the slow component of the delayed rectifier potassium current, I <jats:sub>Ks</jats:sub> . Cardiomyocytes (CMs) from two independent sets of patient-derived and engineered hiPSCs showed electrophysiological defects that reflect the severity of the condition in patients. Our work allowed better understanding of the mechanisms of recessive inheritance. Furthermore, JLNS-CMs showed increased sensitivity to proarrhythmic drugs, which could be rescued pharmacologically, demonstrating the potential of hiPSC-CMs in drug testing. </jats:p>
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.1419553111