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The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 105, 2008, 29, S. 9970-9975 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Dong, Zhicheng Han, Meng-Hsuan Fedoroff, Nina Dong, Zhicheng Han, Meng-Hsuan Fedoroff, Nina |
|---|---|
| author |
Dong, Zhicheng Han, Meng-Hsuan Fedoroff, Nina |
| spellingShingle |
Dong, Zhicheng Han, Meng-Hsuan Fedoroff, Nina Proceedings of the National Academy of Sciences The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 Multidisciplinary |
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dong, zhicheng |
| spelling |
Dong, Zhicheng Han, Meng-Hsuan Fedoroff, Nina 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0803356105 <jats:p> The results of genetic studies in <jats:italic>Arabidopsis</jats:italic> indicate that three proteins, the RNase III DICER-Like1 (DCL1), the dsRNA-binding protein HYPONASTIC LEAVES1 (HYL1), and the C2H2 Zn-finger protein SERRATE (SE), are required for the accurate processing of microRNA (miRNA) precursors in the plant cell nucleus. To elucidate the biochemical mechanism of miRNA processing, we developed an <jats:italic>in vitro</jats:italic> miRNA processing assay using purified recombinant proteins. We find that DCL1 alone releases 21-nt short RNAs from dsRNA as well as synthetic miR167b precursor RNAs. However, correctly processed miRNAs constitute a minority of the cleavage products. We show that recombinant HYL1 and SE proteins accelerate the rate of DCL1-mediated cleavage of pre- and pri-miR167b substrates and promote accurate processing. </jats:p> The RNA-binding proteins HYL1 and SE promote accurate <i>in vitro</i> processing of pri-miRNA by DCL1 Proceedings of the National Academy of Sciences |
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| title |
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_unstemmed |
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_full |
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_fullStr |
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_full_unstemmed |
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_short |
The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_sort |
the rna-binding proteins hyl1 and se promote accurate
<i>in vitro</i>
processing of pri-mirna by dcl1 |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.0803356105 |
| publishDate |
2008 |
| physical |
9970-9975 |
| description |
<jats:p>
The results of genetic studies in
<jats:italic>Arabidopsis</jats:italic>
indicate that three proteins, the RNase III DICER-Like1 (DCL1), the dsRNA-binding protein HYPONASTIC LEAVES1 (HYL1), and the C2H2 Zn-finger protein SERRATE (SE), are required for the accurate processing of microRNA (miRNA) precursors in the plant cell nucleus. To elucidate the biochemical mechanism of miRNA processing, we developed an
<jats:italic>in vitro</jats:italic>
miRNA processing assay using purified recombinant proteins. We find that DCL1 alone releases 21-nt short RNAs from dsRNA as well as synthetic miR167b precursor RNAs. However, correctly processed miRNAs constitute a minority of the cleavage products. We show that recombinant HYL1 and SE proteins accelerate the rate of DCL1-mediated cleavage of pre- and pri-miR167b substrates and promote accurate processing.
</jats:p> |
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| author | Dong, Zhicheng, Han, Meng-Hsuan, Fedoroff, Nina |
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| description | <jats:p> The results of genetic studies in <jats:italic>Arabidopsis</jats:italic> indicate that three proteins, the RNase III DICER-Like1 (DCL1), the dsRNA-binding protein HYPONASTIC LEAVES1 (HYL1), and the C2H2 Zn-finger protein SERRATE (SE), are required for the accurate processing of microRNA (miRNA) precursors in the plant cell nucleus. To elucidate the biochemical mechanism of miRNA processing, we developed an <jats:italic>in vitro</jats:italic> miRNA processing assay using purified recombinant proteins. We find that DCL1 alone releases 21-nt short RNAs from dsRNA as well as synthetic miR167b precursor RNAs. However, correctly processed miRNAs constitute a minority of the cleavage products. We show that recombinant HYL1 and SE proteins accelerate the rate of DCL1-mediated cleavage of pre- and pri-miR167b substrates and promote accurate processing. </jats:p> |
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| imprint_str_mv | Proceedings of the National Academy of Sciences, 2008 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| spelling | Dong, Zhicheng Han, Meng-Hsuan Fedoroff, Nina 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0803356105 <jats:p> The results of genetic studies in <jats:italic>Arabidopsis</jats:italic> indicate that three proteins, the RNase III DICER-Like1 (DCL1), the dsRNA-binding protein HYPONASTIC LEAVES1 (HYL1), and the C2H2 Zn-finger protein SERRATE (SE), are required for the accurate processing of microRNA (miRNA) precursors in the plant cell nucleus. To elucidate the biochemical mechanism of miRNA processing, we developed an <jats:italic>in vitro</jats:italic> miRNA processing assay using purified recombinant proteins. We find that DCL1 alone releases 21-nt short RNAs from dsRNA as well as synthetic miR167b precursor RNAs. However, correctly processed miRNAs constitute a minority of the cleavage products. We show that recombinant HYL1 and SE proteins accelerate the rate of DCL1-mediated cleavage of pre- and pri-miR167b substrates and promote accurate processing. </jats:p> The RNA-binding proteins HYL1 and SE promote accurate <i>in vitro</i> processing of pri-miRNA by DCL1 Proceedings of the National Academy of Sciences |
| spellingShingle | Dong, Zhicheng, Han, Meng-Hsuan, Fedoroff, Nina, Proceedings of the National Academy of Sciences, The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1, Multidisciplinary |
| title | The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_full | The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_fullStr | The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_full_unstemmed | The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_short | The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| title_sort | the rna-binding proteins hyl1 and se promote accurate <i>in vitro</i> processing of pri-mirna by dcl1 |
| title_unstemmed | The RNA-binding proteins HYL1 and SE promote accurate in vitro processing of pri-miRNA by DCL1 |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.0803356105 |