Eintrag weiter verarbeiten
Efficient active transport of gene nanocarriers to the cell nucleus
Gespeichert in:
| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Proceedings of the National Academy of Sciences, 100, 2003, 7, S. 3878-3882 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Suh, Junghae Wirtz, Denis Hanes, Justin Suh, Junghae Wirtz, Denis Hanes, Justin |
|---|---|
| author |
Suh, Junghae Wirtz, Denis Hanes, Justin |
| spellingShingle |
Suh, Junghae Wirtz, Denis Hanes, Justin Proceedings of the National Academy of Sciences Efficient active transport of gene nanocarriers to the cell nucleus Multidisciplinary |
| author_sort |
suh, junghae |
| spelling |
Suh, Junghae Wirtz, Denis Hanes, Justin 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0636277100 <jats:p>The intracellular transport of therapeutic gene carriers is poorly understood, limiting the rational design of efficient new vectors. We used live-cell real-time multiple particle tracking to quantify the intracellular transport of hundreds of individual nonviral DNA nanocarriers with 5-nm and 33-ms resolution. Unexpected parallels between several of nature's most efficient DNA viruses and nonviral polyethylenimine/DNA nanocomplexes were revealed to include motor protein-driven transport through the cytoplasm toward the nucleus on microtubules. Active gene carrier transport led to efficient perinuclear accumulation within minutes. The results provide direct evidence to dispute the common belief that the efficiency of nonviral gene carriers is dramatically reduced because of the need for their relatively slow random diffusion through the cell cytoplasm to the nucleus and, instead, focuses the attention of rational carrier design on overcoming barriers downstream of perinuclear accumulation.</jats:p> Efficient active transport of gene nanocarriers to the cell nucleus Proceedings of the National Academy of Sciences |
| doi_str_mv |
10.1073/pnas.0636277100 |
| facet_avail |
Online Free |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjA2MzYyNzcxMDA |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjA2MzYyNzcxMDA |
| institution |
DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Rs1 DE-Pl11 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 |
| imprint |
Proceedings of the National Academy of Sciences, 2003 |
| imprint_str_mv |
Proceedings of the National Academy of Sciences, 2003 |
| issn |
0027-8424 1091-6490 |
| issn_str_mv |
0027-8424 1091-6490 |
| language |
English |
| mega_collection |
Proceedings of the National Academy of Sciences (CrossRef) |
| match_str |
suh2003efficientactivetransportofgenenanocarrierstothecellnucleus |
| publishDateSort |
2003 |
| publisher |
Proceedings of the National Academy of Sciences |
| recordtype |
ai |
| record_format |
ai |
| series |
Proceedings of the National Academy of Sciences |
| source_id |
49 |
| title |
Efficient active transport of gene nanocarriers to the cell nucleus |
| title_unstemmed |
Efficient active transport of gene nanocarriers to the cell nucleus |
| title_full |
Efficient active transport of gene nanocarriers to the cell nucleus |
| title_fullStr |
Efficient active transport of gene nanocarriers to the cell nucleus |
| title_full_unstemmed |
Efficient active transport of gene nanocarriers to the cell nucleus |
| title_short |
Efficient active transport of gene nanocarriers to the cell nucleus |
| title_sort |
efficient active transport of gene nanocarriers to the cell nucleus |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.0636277100 |
| publishDate |
2003 |
| physical |
3878-3882 |
| description |
<jats:p>The intracellular transport of therapeutic gene carriers is poorly understood, limiting the rational design of efficient new vectors. We used live-cell real-time multiple particle tracking to quantify the intracellular transport of hundreds of individual nonviral DNA nanocarriers with 5-nm and 33-ms resolution. Unexpected parallels between several of nature's most efficient DNA viruses and nonviral polyethylenimine/DNA nanocomplexes were revealed to include motor protein-driven transport through the cytoplasm toward the nucleus on microtubules. Active gene carrier transport led to efficient perinuclear accumulation within minutes. The results provide direct evidence to dispute the common belief that the efficiency of nonviral gene carriers is dramatically reduced because of the need for their relatively slow random diffusion through the cell cytoplasm to the nucleus and, instead, focuses the attention of rational carrier design on overcoming barriers downstream of perinuclear accumulation.</jats:p> |
| container_issue |
7 |
| container_start_page |
3878 |
| container_title |
Proceedings of the National Academy of Sciences |
| container_volume |
100 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792339396907761675 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T15:47:28.423Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Efficient+active+transport+of+gene+nanocarriers+to+the+cell+nucleus&rft.date=2003-04-01&genre=article&issn=1091-6490&volume=100&issue=7&spage=3878&epage=3882&pages=3878-3882&jtitle=Proceedings+of+the+National+Academy+of+Sciences&atitle=Efficient+active+transport+of+gene+nanocarriers+to+the+cell+nucleus&aulast=Hanes&aufirst=Justin&rft_id=info%3Adoi%2F10.1073%2Fpnas.0636277100&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792339396907761675 |
| author | Suh, Junghae, Wirtz, Denis, Hanes, Justin |
| author_facet | Suh, Junghae, Wirtz, Denis, Hanes, Justin, Suh, Junghae, Wirtz, Denis, Hanes, Justin |
| author_sort | suh, junghae |
| container_issue | 7 |
| container_start_page | 3878 |
| container_title | Proceedings of the National Academy of Sciences |
| container_volume | 100 |
| description | <jats:p>The intracellular transport of therapeutic gene carriers is poorly understood, limiting the rational design of efficient new vectors. We used live-cell real-time multiple particle tracking to quantify the intracellular transport of hundreds of individual nonviral DNA nanocarriers with 5-nm and 33-ms resolution. Unexpected parallels between several of nature's most efficient DNA viruses and nonviral polyethylenimine/DNA nanocomplexes were revealed to include motor protein-driven transport through the cytoplasm toward the nucleus on microtubules. Active gene carrier transport led to efficient perinuclear accumulation within minutes. The results provide direct evidence to dispute the common belief that the efficiency of nonviral gene carriers is dramatically reduced because of the need for their relatively slow random diffusion through the cell cytoplasm to the nucleus and, instead, focuses the attention of rational carrier design on overcoming barriers downstream of perinuclear accumulation.</jats:p> |
| doi_str_mv | 10.1073/pnas.0636277100 |
| facet_avail | Online, Free |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA3My9wbmFzLjA2MzYyNzcxMDA |
| imprint | Proceedings of the National Academy of Sciences, 2003 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 2003 |
| institution | DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Rs1, DE-Pl11, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
| issn | 0027-8424, 1091-6490 |
| issn_str_mv | 0027-8424, 1091-6490 |
| language | English |
| last_indexed | 2024-03-01T15:47:28.423Z |
| match_str | suh2003efficientactivetransportofgenenanocarrierstothecellnucleus |
| mega_collection | Proceedings of the National Academy of Sciences (CrossRef) |
| physical | 3878-3882 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | Proceedings of the National Academy of Sciences |
| record_format | ai |
| recordtype | ai |
| series | Proceedings of the National Academy of Sciences |
| source_id | 49 |
| spelling | Suh, Junghae Wirtz, Denis Hanes, Justin 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0636277100 <jats:p>The intracellular transport of therapeutic gene carriers is poorly understood, limiting the rational design of efficient new vectors. We used live-cell real-time multiple particle tracking to quantify the intracellular transport of hundreds of individual nonviral DNA nanocarriers with 5-nm and 33-ms resolution. Unexpected parallels between several of nature's most efficient DNA viruses and nonviral polyethylenimine/DNA nanocomplexes were revealed to include motor protein-driven transport through the cytoplasm toward the nucleus on microtubules. Active gene carrier transport led to efficient perinuclear accumulation within minutes. The results provide direct evidence to dispute the common belief that the efficiency of nonviral gene carriers is dramatically reduced because of the need for their relatively slow random diffusion through the cell cytoplasm to the nucleus and, instead, focuses the attention of rational carrier design on overcoming barriers downstream of perinuclear accumulation.</jats:p> Efficient active transport of gene nanocarriers to the cell nucleus Proceedings of the National Academy of Sciences |
| spellingShingle | Suh, Junghae, Wirtz, Denis, Hanes, Justin, Proceedings of the National Academy of Sciences, Efficient active transport of gene nanocarriers to the cell nucleus, Multidisciplinary |
| title | Efficient active transport of gene nanocarriers to the cell nucleus |
| title_full | Efficient active transport of gene nanocarriers to the cell nucleus |
| title_fullStr | Efficient active transport of gene nanocarriers to the cell nucleus |
| title_full_unstemmed | Efficient active transport of gene nanocarriers to the cell nucleus |
| title_short | Efficient active transport of gene nanocarriers to the cell nucleus |
| title_sort | efficient active transport of gene nanocarriers to the cell nucleus |
| title_unstemmed | Efficient active transport of gene nanocarriers to the cell nucleus |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.0636277100 |