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A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
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| Personen und Körperschaften: | , |
| In: | Proceedings of the National Academy of Sciences, 101, 2004, 36, S. 13374-13379 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
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| Schlagwörter: |
| author_facet |
Kim, Jeong Hoe Kende, Hans Kim, Jeong Hoe Kende, Hans |
|---|---|
| author |
Kim, Jeong Hoe Kende, Hans |
| spellingShingle |
Kim, Jeong Hoe Kende, Hans Proceedings of the National Academy of Sciences A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis Multidisciplinary |
| author_sort |
kim, jeong hoe |
| spelling |
Kim, Jeong Hoe Kende, Hans 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0405450101 <jats:p> Previously, we described the <jats:italic>AtGRF</jats:italic> [ <jats:italic>Arabidopsis thaliana</jats:italic> growth-regulating factor (GRF)] gene family, which encodes putative transcription factors that play a regulatory role in growth and development of leaves and cotyledons. We demonstrate here that the C-terminal region of GRF proteins has transactivation activity. In search of partner proteins for GRF1, we identified another gene family, GRF-interacting factor ( <jats:italic>GIF</jats:italic> ), which comprises three members. Sequence and molecular analysis showed that GIF1 is a functional homolog of the human SYT transcription coactivator. We found that the N-terminal region of GIF1 protein was involved in the interaction with GRF1. To understand the biological function of GIF1, we isolated a loss-of-function mutant of <jats:italic>GIF1</jats:italic> and prepared transgenic plants subject to <jats:italic>GIF1</jats:italic> -specific RNA interference. Like <jats:italic>grf</jats:italic> mutants, the <jats:italic>gif1</jats:italic> mutant and transgenic plants developed narrower leaves and petals than did wild-type plants, and combinations of <jats:italic>gif1</jats:italic> and <jats:italic>grf</jats:italic> mutations showed a cooperative effect. The narrow leaf phenotype of <jats:italic>gif1</jats:italic> , as well as that of the <jats:italic>grf</jats:italic> triple mutant, was caused by a reduction in cell numbers along the leaf-width axis. We propose that GRF1 and GIF1 act as transcription activator and coactivator, respectively, and that they are part of a complex involved in regulating the growth and shape of leaves and petals. </jats:p> A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in <i>Arabidopsis</i> Proceedings of the National Academy of Sciences |
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Proceedings of the National Academy of Sciences, 2004 |
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| title |
A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_unstemmed |
A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_full |
A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_fullStr |
A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_full_unstemmed |
A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_short |
A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_sort |
a transcriptional coactivator, atgif1, is involved in regulating leaf growth and morphology in
<i>arabidopsis</i> |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.0405450101 |
| publishDate |
2004 |
| physical |
13374-13379 |
| description |
<jats:p>
Previously, we described the
<jats:italic>AtGRF</jats:italic>
[
<jats:italic>Arabidopsis thaliana</jats:italic>
growth-regulating factor (GRF)] gene family, which encodes putative transcription factors that play a regulatory role in growth and development of leaves and cotyledons. We demonstrate here that the C-terminal region of GRF proteins has transactivation activity. In search of partner proteins for GRF1, we identified another gene family, GRF-interacting factor (
<jats:italic>GIF</jats:italic>
), which comprises three members. Sequence and molecular analysis showed that GIF1 is a functional homolog of the human SYT transcription coactivator. We found that the N-terminal region of GIF1 protein was involved in the interaction with GRF1. To understand the biological function of GIF1, we isolated a loss-of-function mutant of
<jats:italic>GIF1</jats:italic>
and prepared transgenic plants subject to
<jats:italic>GIF1</jats:italic>
-specific RNA interference. Like
<jats:italic>grf</jats:italic>
mutants, the
<jats:italic>gif1</jats:italic>
mutant and transgenic plants developed narrower leaves and petals than did wild-type plants, and combinations of
<jats:italic>gif1</jats:italic>
and
<jats:italic>grf</jats:italic>
mutations showed a cooperative effect. The narrow leaf phenotype of
<jats:italic>gif1</jats:italic>
, as well as that of the
<jats:italic>grf</jats:italic>
triple mutant, was caused by a reduction in cell numbers along the leaf-width axis. We propose that GRF1 and GIF1 act as transcription activator and coactivator, respectively, and that they are part of a complex involved in regulating the growth and shape of leaves and petals.
</jats:p> |
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| author | Kim, Jeong Hoe, Kende, Hans |
| author_facet | Kim, Jeong Hoe, Kende, Hans, Kim, Jeong Hoe, Kende, Hans |
| author_sort | kim, jeong hoe |
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| container_title | Proceedings of the National Academy of Sciences |
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| description | <jats:p> Previously, we described the <jats:italic>AtGRF</jats:italic> [ <jats:italic>Arabidopsis thaliana</jats:italic> growth-regulating factor (GRF)] gene family, which encodes putative transcription factors that play a regulatory role in growth and development of leaves and cotyledons. We demonstrate here that the C-terminal region of GRF proteins has transactivation activity. In search of partner proteins for GRF1, we identified another gene family, GRF-interacting factor ( <jats:italic>GIF</jats:italic> ), which comprises three members. Sequence and molecular analysis showed that GIF1 is a functional homolog of the human SYT transcription coactivator. We found that the N-terminal region of GIF1 protein was involved in the interaction with GRF1. To understand the biological function of GIF1, we isolated a loss-of-function mutant of <jats:italic>GIF1</jats:italic> and prepared transgenic plants subject to <jats:italic>GIF1</jats:italic> -specific RNA interference. Like <jats:italic>grf</jats:italic> mutants, the <jats:italic>gif1</jats:italic> mutant and transgenic plants developed narrower leaves and petals than did wild-type plants, and combinations of <jats:italic>gif1</jats:italic> and <jats:italic>grf</jats:italic> mutations showed a cooperative effect. The narrow leaf phenotype of <jats:italic>gif1</jats:italic> , as well as that of the <jats:italic>grf</jats:italic> triple mutant, was caused by a reduction in cell numbers along the leaf-width axis. We propose that GRF1 and GIF1 act as transcription activator and coactivator, respectively, and that they are part of a complex involved in regulating the growth and shape of leaves and petals. </jats:p> |
| doi_str_mv | 10.1073/pnas.0405450101 |
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| imprint | Proceedings of the National Academy of Sciences, 2004 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 2004 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Zi4, DE-Gla1, DE-15, DE-Pl11, DE-Rs1, DE-14, DE-105, DE-Ch1, DE-L229 |
| issn | 0027-8424, 1091-6490 |
| issn_str_mv | 0027-8424, 1091-6490 |
| language | English |
| last_indexed | 2024-03-01T17:43:23.847Z |
| match_str | kim2004atranscriptionalcoactivatoratgif1isinvolvedinregulatingleafgrowthandmorphologyinarabidopsis |
| mega_collection | Proceedings of the National Academy of Sciences (CrossRef) |
| physical | 13374-13379 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | Proceedings of the National Academy of Sciences |
| record_format | ai |
| recordtype | ai |
| series | Proceedings of the National Academy of Sciences |
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| spelling | Kim, Jeong Hoe Kende, Hans 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0405450101 <jats:p> Previously, we described the <jats:italic>AtGRF</jats:italic> [ <jats:italic>Arabidopsis thaliana</jats:italic> growth-regulating factor (GRF)] gene family, which encodes putative transcription factors that play a regulatory role in growth and development of leaves and cotyledons. We demonstrate here that the C-terminal region of GRF proteins has transactivation activity. In search of partner proteins for GRF1, we identified another gene family, GRF-interacting factor ( <jats:italic>GIF</jats:italic> ), which comprises three members. Sequence and molecular analysis showed that GIF1 is a functional homolog of the human SYT transcription coactivator. We found that the N-terminal region of GIF1 protein was involved in the interaction with GRF1. To understand the biological function of GIF1, we isolated a loss-of-function mutant of <jats:italic>GIF1</jats:italic> and prepared transgenic plants subject to <jats:italic>GIF1</jats:italic> -specific RNA interference. Like <jats:italic>grf</jats:italic> mutants, the <jats:italic>gif1</jats:italic> mutant and transgenic plants developed narrower leaves and petals than did wild-type plants, and combinations of <jats:italic>gif1</jats:italic> and <jats:italic>grf</jats:italic> mutations showed a cooperative effect. The narrow leaf phenotype of <jats:italic>gif1</jats:italic> , as well as that of the <jats:italic>grf</jats:italic> triple mutant, was caused by a reduction in cell numbers along the leaf-width axis. We propose that GRF1 and GIF1 act as transcription activator and coactivator, respectively, and that they are part of a complex involved in regulating the growth and shape of leaves and petals. </jats:p> A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in <i>Arabidopsis</i> Proceedings of the National Academy of Sciences |
| spellingShingle | Kim, Jeong Hoe, Kende, Hans, Proceedings of the National Academy of Sciences, A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis, Multidisciplinary |
| title | A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_full | A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_fullStr | A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_full_unstemmed | A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_short | A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| title_sort | a transcriptional coactivator, atgif1, is involved in regulating leaf growth and morphology in <i>arabidopsis</i> |
| title_unstemmed | A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.0405450101 |