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Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions
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| Zeitschriftentitel: | Proceedings of the National Academy of Sciences |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | Proceedings of the National Academy of Sciences, 101, 2004, 32, S. 11634-11639 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Proceedings of the National Academy of Sciences
|
| Schlagwörter: |
| author_facet |
Xu, Qing-Hua Gaylord, Brent S. Wang, Shu Bazan, Guillermo C. Moses, Daniel Heeger, Alan J. Xu, Qing-Hua Gaylord, Brent S. Wang, Shu Bazan, Guillermo C. Moses, Daniel Heeger, Alan J. |
|---|---|
| author |
Xu, Qing-Hua Gaylord, Brent S. Wang, Shu Bazan, Guillermo C. Moses, Daniel Heeger, Alan J. |
| spellingShingle |
Xu, Qing-Hua Gaylord, Brent S. Wang, Shu Bazan, Guillermo C. Moses, Daniel Heeger, Alan J. Proceedings of the National Academy of Sciences Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions Multidisciplinary |
| author_sort |
xu, qing-hua |
| spelling |
Xu, Qing-Hua Gaylord, Brent S. Wang, Shu Bazan, Guillermo C. Moses, Daniel Heeger, Alan J. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0404574101 <jats:p>We have investigated the energy transfer processes in DNA sequence detection by using cationic conjugated polymers and peptide nucleic acid (PNA) probes with ultrafast pump-dump-emission spectroscopy. Pump-dump-emission spectroscopy provides femtosecond temporal resolution and high sensitivity and avoids interference from the solvent response. The energy transfer from donor (the conjugated polymer) to acceptor (a fluorescent molecule attached to a PNA terminus) has been time resolved. The results indicate that both electrostatic and hydrophobic interactions contribute to the formation of cationic conjugated polymers/PNA-C/DNA complexes. The two interactions result in two different binding conformations. This picture is supported by the average donor–acceptor separations as estimated from time-resolved and steady-state measurements. Electrostatic interactions dominate at low concentrations and in mixed solvents.</jats:p> Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions Proceedings of the National Academy of Sciences |
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Proceedings of the National Academy of Sciences, 2004 |
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Proceedings of the National Academy of Sciences, 2004 |
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Proceedings of the National Academy of Sciences |
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| title |
Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_unstemmed |
Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_full |
Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_fullStr |
Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_full_unstemmed |
Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_short |
Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_sort |
time-resolved energy transfer in dna sequence detection using water-soluble conjugated polymers: the role of electrostatic and hydrophobic interactions |
| topic |
Multidisciplinary |
| url |
http://dx.doi.org/10.1073/pnas.0404574101 |
| publishDate |
2004 |
| physical |
11634-11639 |
| description |
<jats:p>We have investigated the energy transfer processes in DNA sequence detection by using cationic conjugated polymers and peptide nucleic acid (PNA) probes with ultrafast pump-dump-emission spectroscopy. Pump-dump-emission spectroscopy provides femtosecond temporal resolution and high sensitivity and avoids interference from the solvent response. The energy transfer from donor (the conjugated polymer) to acceptor (a fluorescent molecule attached to a PNA terminus) has been time resolved. The results indicate that both electrostatic and hydrophobic interactions contribute to the formation of cationic conjugated polymers/PNA-C/DNA complexes. The two interactions result in two different binding conformations. This picture is supported by the average donor–acceptor separations as estimated from time-resolved and steady-state measurements. Electrostatic interactions dominate at low concentrations and in mixed solvents.</jats:p> |
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| author | Xu, Qing-Hua, Gaylord, Brent S., Wang, Shu, Bazan, Guillermo C., Moses, Daniel, Heeger, Alan J. |
| author_facet | Xu, Qing-Hua, Gaylord, Brent S., Wang, Shu, Bazan, Guillermo C., Moses, Daniel, Heeger, Alan J., Xu, Qing-Hua, Gaylord, Brent S., Wang, Shu, Bazan, Guillermo C., Moses, Daniel, Heeger, Alan J. |
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| container_title | Proceedings of the National Academy of Sciences |
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| description | <jats:p>We have investigated the energy transfer processes in DNA sequence detection by using cationic conjugated polymers and peptide nucleic acid (PNA) probes with ultrafast pump-dump-emission spectroscopy. Pump-dump-emission spectroscopy provides femtosecond temporal resolution and high sensitivity and avoids interference from the solvent response. The energy transfer from donor (the conjugated polymer) to acceptor (a fluorescent molecule attached to a PNA terminus) has been time resolved. The results indicate that both electrostatic and hydrophobic interactions contribute to the formation of cationic conjugated polymers/PNA-C/DNA complexes. The two interactions result in two different binding conformations. This picture is supported by the average donor–acceptor separations as estimated from time-resolved and steady-state measurements. Electrostatic interactions dominate at low concentrations and in mixed solvents.</jats:p> |
| doi_str_mv | 10.1073/pnas.0404574101 |
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| imprint | Proceedings of the National Academy of Sciences, 2004 |
| imprint_str_mv | Proceedings of the National Academy of Sciences, 2004 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
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| physical | 11634-11639 |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | Proceedings of the National Academy of Sciences |
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| recordtype | ai |
| series | Proceedings of the National Academy of Sciences |
| source_id | 49 |
| spelling | Xu, Qing-Hua Gaylord, Brent S. Wang, Shu Bazan, Guillermo C. Moses, Daniel Heeger, Alan J. 0027-8424 1091-6490 Proceedings of the National Academy of Sciences Multidisciplinary http://dx.doi.org/10.1073/pnas.0404574101 <jats:p>We have investigated the energy transfer processes in DNA sequence detection by using cationic conjugated polymers and peptide nucleic acid (PNA) probes with ultrafast pump-dump-emission spectroscopy. Pump-dump-emission spectroscopy provides femtosecond temporal resolution and high sensitivity and avoids interference from the solvent response. The energy transfer from donor (the conjugated polymer) to acceptor (a fluorescent molecule attached to a PNA terminus) has been time resolved. The results indicate that both electrostatic and hydrophobic interactions contribute to the formation of cationic conjugated polymers/PNA-C/DNA complexes. The two interactions result in two different binding conformations. This picture is supported by the average donor–acceptor separations as estimated from time-resolved and steady-state measurements. Electrostatic interactions dominate at low concentrations and in mixed solvents.</jats:p> Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions Proceedings of the National Academy of Sciences |
| spellingShingle | Xu, Qing-Hua, Gaylord, Brent S., Wang, Shu, Bazan, Guillermo C., Moses, Daniel, Heeger, Alan J., Proceedings of the National Academy of Sciences, Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions, Multidisciplinary |
| title | Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_full | Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_fullStr | Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_full_unstemmed | Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_short | Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| title_sort | time-resolved energy transfer in dna sequence detection using water-soluble conjugated polymers: the role of electrostatic and hydrophobic interactions |
| title_unstemmed | Time-resolved energy transfer in DNA sequence detection using water-soluble conjugated polymers: The role of electrostatic and hydrophobic interactions |
| topic | Multidisciplinary |
| url | http://dx.doi.org/10.1073/pnas.0404574101 |