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Microfluidic immunomagnetic cell separation using integrated permanent micromagnets
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| Zeitschriftentitel: | Biomicrofluidics |
|---|---|
| Personen und Körperschaften: | , , , , , , , , |
| In: | Biomicrofluidics, 7, 2013, 5 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
AIP Publishing
|
| Schlagwörter: |
| author_facet |
Osman, O. Toru, S. Dumas-Bouchiat, F. Dempsey, N. M. Haddour, N. Zanini, L.-F. Buret, F. Reyne, G. Frénéa-Robin, M. Osman, O. Toru, S. Dumas-Bouchiat, F. Dempsey, N. M. Haddour, N. Zanini, L.-F. Buret, F. Reyne, G. Frénéa-Robin, M. |
|---|---|
| author |
Osman, O. Toru, S. Dumas-Bouchiat, F. Dempsey, N. M. Haddour, N. Zanini, L.-F. Buret, F. Reyne, G. Frénéa-Robin, M. |
| spellingShingle |
Osman, O. Toru, S. Dumas-Bouchiat, F. Dempsey, N. M. Haddour, N. Zanini, L.-F. Buret, F. Reyne, G. Frénéa-Robin, M. Biomicrofluidics Microfluidic immunomagnetic cell separation using integrated permanent micromagnets Condensed Matter Physics General Materials Science Fluid Flow and Transfer Processes Colloid and Surface Chemistry Biomedical Engineering |
| author_sort |
osman, o. |
| spelling |
Osman, O. Toru, S. Dumas-Bouchiat, F. Dempsey, N. M. Haddour, N. Zanini, L.-F. Buret, F. Reyne, G. Frénéa-Robin, M. 1932-1058 AIP Publishing Condensed Matter Physics General Materials Science Fluid Flow and Transfer Processes Colloid and Surface Chemistry Biomedical Engineering http://dx.doi.org/10.1063/1.4825395 <jats:p>In this paper, we demonstrate the possibility to trap and sort labeled cells under flow conditions using a microfluidic device with an integrated flat micro-patterned hard magnetic film. The proposed technique is illustrated using a cell suspension containing a mixture of Jurkat cells and HEK (Human Embryonic Kidney) 293 cells. Prior to sorting experiments, the Jurkat cells were specifically labeled with immunomagnetic nanoparticles, while the HEK 293 cells were unlabeled. Droplet-based experiments demonstrated that the Jurkat cells were attracted to regions of maximum stray field flux density while the HEK 293 cells settled in random positions. When the mixture was passed through a polydimethylsiloxane (PDMS) microfluidic channel containing integrated micromagnets, the labeled Jurkat cells were selectively trapped under fluid flow, while the HEK cells were eluted towards the device outlet. Increasing the flow rate produced a second eluate much enriched in Jurkat cells, as revealed by flow cytometry. The separation efficiency of this biocompatible, compact micro-fluidic separation chamber was compared with that obtained using two commercial magnetic cell separation kits.</jats:p> Microfluidic immunomagnetic cell separation using integrated permanent micromagnets Biomicrofluidics |
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10.1063/1.4825395 |
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AIP Publishing |
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Biomicrofluidics |
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| title |
Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_unstemmed |
Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_full |
Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_fullStr |
Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_full_unstemmed |
Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_short |
Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_sort |
microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| topic |
Condensed Matter Physics General Materials Science Fluid Flow and Transfer Processes Colloid and Surface Chemistry Biomedical Engineering |
| url |
http://dx.doi.org/10.1063/1.4825395 |
| publishDate |
2013 |
| physical |
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| description |
<jats:p>In this paper, we demonstrate the possibility to trap and sort labeled cells under flow conditions using a microfluidic device with an integrated flat micro-patterned hard magnetic film. The proposed technique is illustrated using a cell suspension containing a mixture of Jurkat cells and HEK (Human Embryonic Kidney) 293 cells. Prior to sorting experiments, the Jurkat cells were specifically labeled with immunomagnetic nanoparticles, while the HEK 293 cells were unlabeled. Droplet-based experiments demonstrated that the Jurkat cells were attracted to regions of maximum stray field flux density while the HEK 293 cells settled in random positions. When the mixture was passed through a polydimethylsiloxane (PDMS) microfluidic channel containing integrated micromagnets, the labeled Jurkat cells were selectively trapped under fluid flow, while the HEK cells were eluted towards the device outlet. Increasing the flow rate produced a second eluate much enriched in Jurkat cells, as revealed by flow cytometry. The separation efficiency of this biocompatible, compact micro-fluidic separation chamber was compared with that obtained using two commercial magnetic cell separation kits.</jats:p> |
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| author | Osman, O., Toru, S., Dumas-Bouchiat, F., Dempsey, N. M., Haddour, N., Zanini, L.-F., Buret, F., Reyne, G., Frénéa-Robin, M. |
| author_facet | Osman, O., Toru, S., Dumas-Bouchiat, F., Dempsey, N. M., Haddour, N., Zanini, L.-F., Buret, F., Reyne, G., Frénéa-Robin, M., Osman, O., Toru, S., Dumas-Bouchiat, F., Dempsey, N. M., Haddour, N., Zanini, L.-F., Buret, F., Reyne, G., Frénéa-Robin, M. |
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| description | <jats:p>In this paper, we demonstrate the possibility to trap and sort labeled cells under flow conditions using a microfluidic device with an integrated flat micro-patterned hard magnetic film. The proposed technique is illustrated using a cell suspension containing a mixture of Jurkat cells and HEK (Human Embryonic Kidney) 293 cells. Prior to sorting experiments, the Jurkat cells were specifically labeled with immunomagnetic nanoparticles, while the HEK 293 cells were unlabeled. Droplet-based experiments demonstrated that the Jurkat cells were attracted to regions of maximum stray field flux density while the HEK 293 cells settled in random positions. When the mixture was passed through a polydimethylsiloxane (PDMS) microfluidic channel containing integrated micromagnets, the labeled Jurkat cells were selectively trapped under fluid flow, while the HEK cells were eluted towards the device outlet. Increasing the flow rate produced a second eluate much enriched in Jurkat cells, as revealed by flow cytometry. The separation efficiency of this biocompatible, compact micro-fluidic separation chamber was compared with that obtained using two commercial magnetic cell separation kits.</jats:p> |
| doi_str_mv | 10.1063/1.4825395 |
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| spelling | Osman, O. Toru, S. Dumas-Bouchiat, F. Dempsey, N. M. Haddour, N. Zanini, L.-F. Buret, F. Reyne, G. Frénéa-Robin, M. 1932-1058 AIP Publishing Condensed Matter Physics General Materials Science Fluid Flow and Transfer Processes Colloid and Surface Chemistry Biomedical Engineering http://dx.doi.org/10.1063/1.4825395 <jats:p>In this paper, we demonstrate the possibility to trap and sort labeled cells under flow conditions using a microfluidic device with an integrated flat micro-patterned hard magnetic film. The proposed technique is illustrated using a cell suspension containing a mixture of Jurkat cells and HEK (Human Embryonic Kidney) 293 cells. Prior to sorting experiments, the Jurkat cells were specifically labeled with immunomagnetic nanoparticles, while the HEK 293 cells were unlabeled. Droplet-based experiments demonstrated that the Jurkat cells were attracted to regions of maximum stray field flux density while the HEK 293 cells settled in random positions. When the mixture was passed through a polydimethylsiloxane (PDMS) microfluidic channel containing integrated micromagnets, the labeled Jurkat cells were selectively trapped under fluid flow, while the HEK cells were eluted towards the device outlet. Increasing the flow rate produced a second eluate much enriched in Jurkat cells, as revealed by flow cytometry. The separation efficiency of this biocompatible, compact micro-fluidic separation chamber was compared with that obtained using two commercial magnetic cell separation kits.</jats:p> Microfluidic immunomagnetic cell separation using integrated permanent micromagnets Biomicrofluidics |
| spellingShingle | Osman, O., Toru, S., Dumas-Bouchiat, F., Dempsey, N. M., Haddour, N., Zanini, L.-F., Buret, F., Reyne, G., Frénéa-Robin, M., Biomicrofluidics, Microfluidic immunomagnetic cell separation using integrated permanent micromagnets, Condensed Matter Physics, General Materials Science, Fluid Flow and Transfer Processes, Colloid and Surface Chemistry, Biomedical Engineering |
| title | Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_full | Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_fullStr | Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_full_unstemmed | Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_short | Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_sort | microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| title_unstemmed | Microfluidic immunomagnetic cell separation using integrated permanent micromagnets |
| topic | Condensed Matter Physics, General Materials Science, Fluid Flow and Transfer Processes, Colloid and Surface Chemistry, Biomedical Engineering |
| url | http://dx.doi.org/10.1063/1.4825395 |