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Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador
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| Zeitschriftentitel: | Plant Pathology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Plant Pathology, 51, 2002, 6, S. 710-719 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Oliva, R. F. Erselius, L. J. Adler, N. E. Forbes, G. A. Oliva, R. F. Erselius, L. J. Adler, N. E. Forbes, G. A. |
|---|---|
| author |
Oliva, R. F. Erselius, L. J. Adler, N. E. Forbes, G. A. |
| spellingShingle |
Oliva, R. F. Erselius, L. J. Adler, N. E. Forbes, G. A. Plant Pathology Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador Horticulture Plant Science Genetics Agronomy and Crop Science |
| author_sort |
oliva, r. f. |
| spelling |
Oliva, R. F. Erselius, L. J. Adler, N. E. Forbes, G. A. 0032-0862 1365-3059 Wiley Horticulture Plant Science Genetics Agronomy and Crop Science http://dx.doi.org/10.1046/j.1365-3059.2002.00784.x <jats:p>To determine the potential of sexual reproduction among host‐adapted populations of <jats:italic>Phytophthora infestans sensu lato</jats:italic> in Ecuador, 13 A1 isolates belonging to clonal lineages US‐1, EC‐1 and EC‐3, and 11 A2 isolates belonging to the clonal lineage EC‐2, were paired on agar plates to induce crossing. In the first experiment, six A1 isolates (three US‐1, two EC‐1 and one EC‐3) were each crossed with three A2 isolates (total = 18 crosses). Matings involving isolates of the EC‐1 lineage produced more oospores of healthy appearance than did matings with isolates of US‐1 or EC‐3. In the second experiment, the oospores of 35 crosses (21 EC‐1 × EC‐2; 10 US‐1 × EC‐2; four EC‐3 × EC‐2) were dispersed on water agar to assess oospore germination. Overall, germination percentages were low. Only one cross produced enough progeny for evaluation. Twenty‐three single‐oospore offspring were isolated and evaluated for mating type; electrophoretic patterns of glucose‐6‐phosphate isomerase (<jats:italic>Gpi</jats:italic>) and peptidase (<jats:italic>Pep</jats:italic>) alloenzyme loci; mitochondrial DNA haplotype; and genomic DNA fingerprint. Multilocus genotype data indicated that all 23 isolates resulted from meiotic recombination. Four progeny with homothallic phenotype appeared to be unstable heterokaryons. Markers at several loci segregated according to simple Mendelian expectations for a diploid organism, but the ratios of three RFLP loci and the <jats:italic>Pep</jats:italic> locus were not consistent with Mendelian expectations. All progeny were nonpathogenic on hosts of the parental genotypes. Reduced mating success and reduced pathogenic fitness of progeny appear to be postmating mechanisms of reproductive isolation in populations of <jats:italic>P. infestans sensu lato</jats:italic> in Ecuador.</jats:p> Potential of sexual reproduction among host‐adapted populations of <i>Phytophthora infestans sensu lato</i> in Ecuador Plant Pathology |
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| title |
Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_unstemmed |
Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_full |
Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_fullStr |
Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_full_unstemmed |
Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_short |
Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_sort |
potential of sexual reproduction among host‐adapted populations of <i>phytophthora infestans sensu lato</i> in ecuador |
| topic |
Horticulture Plant Science Genetics Agronomy and Crop Science |
| url |
http://dx.doi.org/10.1046/j.1365-3059.2002.00784.x |
| publishDate |
2002 |
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710-719 |
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<jats:p>To determine the potential of sexual reproduction among host‐adapted populations of <jats:italic>Phytophthora infestans sensu lato</jats:italic> in Ecuador, 13 A1 isolates belonging to clonal lineages US‐1, EC‐1 and EC‐3, and 11 A2 isolates belonging to the clonal lineage EC‐2, were paired on agar plates to induce crossing. In the first experiment, six A1 isolates (three US‐1, two EC‐1 and one EC‐3) were each crossed with three A2 isolates (total = 18 crosses). Matings involving isolates of the EC‐1 lineage produced more oospores of healthy appearance than did matings with isolates of US‐1 or EC‐3. In the second experiment, the oospores of 35 crosses (21 EC‐1 × EC‐2; 10 US‐1 × EC‐2; four EC‐3 × EC‐2) were dispersed on water agar to assess oospore germination. Overall, germination percentages were low. Only one cross produced enough progeny for evaluation. Twenty‐three single‐oospore offspring were isolated and evaluated for mating type; electrophoretic patterns of glucose‐6‐phosphate isomerase (<jats:italic>Gpi</jats:italic>) and peptidase (<jats:italic>Pep</jats:italic>) alloenzyme loci; mitochondrial DNA haplotype; and genomic DNA fingerprint. Multilocus genotype data indicated that all 23 isolates resulted from meiotic recombination. Four progeny with homothallic phenotype appeared to be unstable heterokaryons. Markers at several loci segregated according to simple Mendelian expectations for a diploid organism, but the ratios of three RFLP loci and the <jats:italic>Pep</jats:italic> locus were not consistent with Mendelian expectations. All progeny were nonpathogenic on hosts of the parental genotypes. Reduced mating success and reduced pathogenic fitness of progeny appear to be postmating mechanisms of reproductive isolation in populations of <jats:italic>P. infestans sensu lato</jats:italic> in Ecuador.</jats:p> |
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| author | Oliva, R. F., Erselius, L. J., Adler, N. E., Forbes, G. A. |
| author_facet | Oliva, R. F., Erselius, L. J., Adler, N. E., Forbes, G. A., Oliva, R. F., Erselius, L. J., Adler, N. E., Forbes, G. A. |
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| description | <jats:p>To determine the potential of sexual reproduction among host‐adapted populations of <jats:italic>Phytophthora infestans sensu lato</jats:italic> in Ecuador, 13 A1 isolates belonging to clonal lineages US‐1, EC‐1 and EC‐3, and 11 A2 isolates belonging to the clonal lineage EC‐2, were paired on agar plates to induce crossing. In the first experiment, six A1 isolates (three US‐1, two EC‐1 and one EC‐3) were each crossed with three A2 isolates (total = 18 crosses). Matings involving isolates of the EC‐1 lineage produced more oospores of healthy appearance than did matings with isolates of US‐1 or EC‐3. In the second experiment, the oospores of 35 crosses (21 EC‐1 × EC‐2; 10 US‐1 × EC‐2; four EC‐3 × EC‐2) were dispersed on water agar to assess oospore germination. Overall, germination percentages were low. Only one cross produced enough progeny for evaluation. Twenty‐three single‐oospore offspring were isolated and evaluated for mating type; electrophoretic patterns of glucose‐6‐phosphate isomerase (<jats:italic>Gpi</jats:italic>) and peptidase (<jats:italic>Pep</jats:italic>) alloenzyme loci; mitochondrial DNA haplotype; and genomic DNA fingerprint. Multilocus genotype data indicated that all 23 isolates resulted from meiotic recombination. Four progeny with homothallic phenotype appeared to be unstable heterokaryons. Markers at several loci segregated according to simple Mendelian expectations for a diploid organism, but the ratios of three RFLP loci and the <jats:italic>Pep</jats:italic> locus were not consistent with Mendelian expectations. All progeny were nonpathogenic on hosts of the parental genotypes. Reduced mating success and reduced pathogenic fitness of progeny appear to be postmating mechanisms of reproductive isolation in populations of <jats:italic>P. infestans sensu lato</jats:italic> in Ecuador.</jats:p> |
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| imprint | Wiley, 2002 |
| imprint_str_mv | Wiley, 2002 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| series | Plant Pathology |
| source_id | 49 |
| spelling | Oliva, R. F. Erselius, L. J. Adler, N. E. Forbes, G. A. 0032-0862 1365-3059 Wiley Horticulture Plant Science Genetics Agronomy and Crop Science http://dx.doi.org/10.1046/j.1365-3059.2002.00784.x <jats:p>To determine the potential of sexual reproduction among host‐adapted populations of <jats:italic>Phytophthora infestans sensu lato</jats:italic> in Ecuador, 13 A1 isolates belonging to clonal lineages US‐1, EC‐1 and EC‐3, and 11 A2 isolates belonging to the clonal lineage EC‐2, were paired on agar plates to induce crossing. In the first experiment, six A1 isolates (three US‐1, two EC‐1 and one EC‐3) were each crossed with three A2 isolates (total = 18 crosses). Matings involving isolates of the EC‐1 lineage produced more oospores of healthy appearance than did matings with isolates of US‐1 or EC‐3. In the second experiment, the oospores of 35 crosses (21 EC‐1 × EC‐2; 10 US‐1 × EC‐2; four EC‐3 × EC‐2) were dispersed on water agar to assess oospore germination. Overall, germination percentages were low. Only one cross produced enough progeny for evaluation. Twenty‐three single‐oospore offspring were isolated and evaluated for mating type; electrophoretic patterns of glucose‐6‐phosphate isomerase (<jats:italic>Gpi</jats:italic>) and peptidase (<jats:italic>Pep</jats:italic>) alloenzyme loci; mitochondrial DNA haplotype; and genomic DNA fingerprint. Multilocus genotype data indicated that all 23 isolates resulted from meiotic recombination. Four progeny with homothallic phenotype appeared to be unstable heterokaryons. Markers at several loci segregated according to simple Mendelian expectations for a diploid organism, but the ratios of three RFLP loci and the <jats:italic>Pep</jats:italic> locus were not consistent with Mendelian expectations. All progeny were nonpathogenic on hosts of the parental genotypes. Reduced mating success and reduced pathogenic fitness of progeny appear to be postmating mechanisms of reproductive isolation in populations of <jats:italic>P. infestans sensu lato</jats:italic> in Ecuador.</jats:p> Potential of sexual reproduction among host‐adapted populations of <i>Phytophthora infestans sensu lato</i> in Ecuador Plant Pathology |
| spellingShingle | Oliva, R. F., Erselius, L. J., Adler, N. E., Forbes, G. A., Plant Pathology, Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador, Horticulture, Plant Science, Genetics, Agronomy and Crop Science |
| title | Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_full | Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_fullStr | Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_full_unstemmed | Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_short | Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| title_sort | potential of sexual reproduction among host‐adapted populations of <i>phytophthora infestans sensu lato</i> in ecuador |
| title_unstemmed | Potential of sexual reproduction among host‐adapted populations of Phytophthora infestans sensu lato in Ecuador |
| topic | Horticulture, Plant Science, Genetics, Agronomy and Crop Science |
| url | http://dx.doi.org/10.1046/j.1365-3059.2002.00784.x |