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Random amplified polymorphic DNA (RAPD) analysis of Phytophthora infestans isolates collected in Canada during 1994 to 1996
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| Zeitschriftentitel: | Plant Pathology |
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| Personen und Körperschaften: | , , , |
| In: | Plant Pathology, 49, 2000, 2, S. 252-260 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| Zusammenfassung: | <jats:p>Mating type, glucose‐6‐phosphate isomerase (<jats:italic>Gpi</jats:italic>) allozyme banding patterns, response to the fungicide metalaxyl and random amplified polymorphic DNA (RAPD) markers were used to characterize genetic variability among 141 Canadian isolates of <jats:italic>Phytophthora infestans</jats:italic> collected between 1994 and 1996. Multiple correspondence analysis of RAPD profiles separated isolates into 21 groups that were not correlated to groups defined by mating type, <jats:italic>Gpi</jats:italic> allozyme banding patterns or response to metalaxyl. Population subdivision analysis showed that 97% of the total genetic variation was found among individuals within populations, compared with 3% among populations. The average similarity coefficient among isolates was 80%. No significant differences in haplotypic diversity were observed among the years under study, but levels of genetic diversity among local populations of <jats:italic>P. infestans</jats:italic> were high (0.76). All classes of response to the fungicide metalaxyl were observed, with 55% of isolates displaying moderate levels of insensitivity. The high level of genetic diversity detected within populations indicates that migration and sexual recombination probably play important roles in the population biology of <jats:italic>P. infestans</jats:italic> in Canada.</jats:p> |
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| Umfang: | 252-260 |
| ISSN: |
0032-0862
1365-3059 |
| DOI: | 10.1046/j.1365-3059.2000.00450.x |