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Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures
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| Zeitschriftentitel: | British Journal of Haematology |
|---|---|
| Personen und Körperschaften: | , , , , , |
| In: | British Journal of Haematology, 119, 2002, 3, S. 792-802 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Peters, Rowayda Leyvraz, Serge Faes‐van't Hull, Eveline Jaunin, Philippe Gerber, Stefan Rollini, Pierre Peters, Rowayda Leyvraz, Serge Faes‐van't Hull, Eveline Jaunin, Philippe Gerber, Stefan Rollini, Pierre |
|---|---|
| author |
Peters, Rowayda Leyvraz, Serge Faes‐van't Hull, Eveline Jaunin, Philippe Gerber, Stefan Rollini, Pierre |
| spellingShingle |
Peters, Rowayda Leyvraz, Serge Faes‐van't Hull, Eveline Jaunin, Philippe Gerber, Stefan Rollini, Pierre British Journal of Haematology Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures Hematology |
| author_sort |
peters, rowayda |
| spelling |
Peters, Rowayda Leyvraz, Serge Faes‐van't Hull, Eveline Jaunin, Philippe Gerber, Stefan Rollini, Pierre 0007-1048 1365-2141 Wiley Hematology http://dx.doi.org/10.1046/j.1365-2141.2002.03873.x <jats:p><jats:bold>Summary.</jats:bold> Successful expansion of haematopoietic cells in <jats:italic>ex vivo</jats:italic> cultures will have important applications in transplantation, gene therapy, immunotherapy and potentially also in the production of non‐haematopoietic cell types. Haematopoietic stem cells (HSC), with their capacity to both self‐renew and differentiate into all blood lineages, represent the ideal target for expansion protocols. However, human HSC are rare, poorly characterized phenotypically and genotypically, and difficult to test functionally. Defining optimal culture parameters for <jats:italic>ex vivo</jats:italic> expansion has been a major challenge. We devised a simple and reproducible stroma‐free liquid culture system enabling long‐term expansion of putative haematopoietic progenitors contained within frozen human fetal liver (FL) crude cell suspensions. Starting from a small number of total nucleated cells, a massive haematopoietic cell expansion, reaching > 10<jats:sup>13</jats:sup>‐fold the input cell number after ∼300 d of culture, was consistently achieved. Cells with a primitive phenotype were present throughout the culture and also underwent a continuous expansion. Moreover, the capacity for multilineage lymphomyeloid differentiation, as well as the recloning capacity of primitive myeloid progenitors, was maintained in culture. With its better proliferative potential as compared with adult sources, FL represents a promising alternative source of HSC and the culture system described here should be useful for clinical applications.</jats:p> Long‐term <i>ex vivo</i> expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures British Journal of Haematology |
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| title |
Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_unstemmed |
Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_full |
Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_fullStr |
Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_full_unstemmed |
Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_short |
Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_sort |
long‐term <i>ex vivo</i> expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| topic |
Hematology |
| url |
http://dx.doi.org/10.1046/j.1365-2141.2002.03873.x |
| publishDate |
2002 |
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792-802 |
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<jats:p><jats:bold>Summary.</jats:bold> Successful expansion of haematopoietic cells in <jats:italic>ex vivo</jats:italic> cultures will have important applications in transplantation, gene therapy, immunotherapy and potentially also in the production of non‐haematopoietic cell types. Haematopoietic stem cells (HSC), with their capacity to both self‐renew and differentiate into all blood lineages, represent the ideal target for expansion protocols. However, human HSC are rare, poorly characterized phenotypically and genotypically, and difficult to test functionally. Defining optimal culture parameters for <jats:italic>ex vivo</jats:italic> expansion has been a major challenge. We devised a simple and reproducible stroma‐free liquid culture system enabling long‐term expansion of putative haematopoietic progenitors contained within frozen human fetal liver (FL) crude cell suspensions. Starting from a small number of total nucleated cells, a massive haematopoietic cell expansion, reaching > 10<jats:sup>13</jats:sup>‐fold the input cell number after ∼300 d of culture, was consistently achieved. Cells with a primitive phenotype were present throughout the culture and also underwent a continuous expansion. Moreover, the capacity for multilineage lymphomyeloid differentiation, as well as the recloning capacity of primitive myeloid progenitors, was maintained in culture. With its better proliferative potential as compared with adult sources, FL represents a promising alternative source of HSC and the culture system described here should be useful for clinical applications.</jats:p> |
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| author | Peters, Rowayda, Leyvraz, Serge, Faes‐van't Hull, Eveline, Jaunin, Philippe, Gerber, Stefan, Rollini, Pierre |
| author_facet | Peters, Rowayda, Leyvraz, Serge, Faes‐van't Hull, Eveline, Jaunin, Philippe, Gerber, Stefan, Rollini, Pierre, Peters, Rowayda, Leyvraz, Serge, Faes‐van't Hull, Eveline, Jaunin, Philippe, Gerber, Stefan, Rollini, Pierre |
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| description | <jats:p><jats:bold>Summary.</jats:bold> Successful expansion of haematopoietic cells in <jats:italic>ex vivo</jats:italic> cultures will have important applications in transplantation, gene therapy, immunotherapy and potentially also in the production of non‐haematopoietic cell types. Haematopoietic stem cells (HSC), with their capacity to both self‐renew and differentiate into all blood lineages, represent the ideal target for expansion protocols. However, human HSC are rare, poorly characterized phenotypically and genotypically, and difficult to test functionally. Defining optimal culture parameters for <jats:italic>ex vivo</jats:italic> expansion has been a major challenge. We devised a simple and reproducible stroma‐free liquid culture system enabling long‐term expansion of putative haematopoietic progenitors contained within frozen human fetal liver (FL) crude cell suspensions. Starting from a small number of total nucleated cells, a massive haematopoietic cell expansion, reaching > 10<jats:sup>13</jats:sup>‐fold the input cell number after ∼300 d of culture, was consistently achieved. Cells with a primitive phenotype were present throughout the culture and also underwent a continuous expansion. Moreover, the capacity for multilineage lymphomyeloid differentiation, as well as the recloning capacity of primitive myeloid progenitors, was maintained in culture. With its better proliferative potential as compared with adult sources, FL represents a promising alternative source of HSC and the culture system described here should be useful for clinical applications.</jats:p> |
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| spelling | Peters, Rowayda Leyvraz, Serge Faes‐van't Hull, Eveline Jaunin, Philippe Gerber, Stefan Rollini, Pierre 0007-1048 1365-2141 Wiley Hematology http://dx.doi.org/10.1046/j.1365-2141.2002.03873.x <jats:p><jats:bold>Summary.</jats:bold> Successful expansion of haematopoietic cells in <jats:italic>ex vivo</jats:italic> cultures will have important applications in transplantation, gene therapy, immunotherapy and potentially also in the production of non‐haematopoietic cell types. Haematopoietic stem cells (HSC), with their capacity to both self‐renew and differentiate into all blood lineages, represent the ideal target for expansion protocols. However, human HSC are rare, poorly characterized phenotypically and genotypically, and difficult to test functionally. Defining optimal culture parameters for <jats:italic>ex vivo</jats:italic> expansion has been a major challenge. We devised a simple and reproducible stroma‐free liquid culture system enabling long‐term expansion of putative haematopoietic progenitors contained within frozen human fetal liver (FL) crude cell suspensions. Starting from a small number of total nucleated cells, a massive haematopoietic cell expansion, reaching > 10<jats:sup>13</jats:sup>‐fold the input cell number after ∼300 d of culture, was consistently achieved. Cells with a primitive phenotype were present throughout the culture and also underwent a continuous expansion. Moreover, the capacity for multilineage lymphomyeloid differentiation, as well as the recloning capacity of primitive myeloid progenitors, was maintained in culture. With its better proliferative potential as compared with adult sources, FL represents a promising alternative source of HSC and the culture system described here should be useful for clinical applications.</jats:p> Long‐term <i>ex vivo</i> expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures British Journal of Haematology |
| spellingShingle | Peters, Rowayda, Leyvraz, Serge, Faes‐van't Hull, Eveline, Jaunin, Philippe, Gerber, Stefan, Rollini, Pierre, British Journal of Haematology, Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures, Hematology |
| title | Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_full | Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_fullStr | Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_full_unstemmed | Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_short | Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_sort | long‐term <i>ex vivo</i> expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| title_unstemmed | Long‐term ex vivo expansion of human fetal liver primitive haematopoietic progenitor cells in stroma‐free cultures |
| topic | Hematology |
| url | http://dx.doi.org/10.1046/j.1365-2141.2002.03873.x |