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l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney
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| Zeitschriftentitel: | Biochemical Journal |
|---|---|
| Personen und Körperschaften: | , |
| In: | Biochemical Journal, 286, 1992, 1, S. 103-110 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Portland Press Ltd.
|
| Schlagwörter: |
| author_facet |
Jessen, H Sheikh, M I Jessen, H Sheikh, M I |
|---|---|
| author |
Jessen, H Sheikh, M I |
| spellingShingle |
Jessen, H Sheikh, M I Biochemical Journal l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney Cell Biology Molecular Biology Biochemistry |
| author_sort |
jessen, h |
| spelling |
Jessen, H Sheikh, M I 0264-6021 1470-8728 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1042/bj2860103 <jats:p>1. The mechanism of the renal transport of L-tryptophan by basolateral and luminal membrane vesicles prepared from either the pars convoluta or the pars recta of the rabbit proximal tubule was studied. The uptake of L-tryptophan by basolateral membrane vesicles from the pars convoluta was found to be an Na(+)-dependent transport event. The Na(+)-conditional influx of the amino acid was stimulated in the presence of an inwardly directed H+ gradient. Lowering the pH without an H+ gradient had no effect, indicating that L-tryptophan is co-transported with H+. 3. On the other hand, no transient accumulation of L-tryptophan was observed in the presence or absence of Na+ in basolateral membrane vesicles from the pars recta. 4. In luminal membrane vesicles from the pars recta, the transient Na(+)-dependent accumulation of L-tryptophan occurred via a dual transport system. In addition, an inwardly directed H+ gradient could drive the uphill transport of L-tryptophan into these vesicles in both the presence and the absence of an Na+ gradient. 5. By contrast, the uptake of L-tryptophan by luminal membrane vesicles from the pars convoluta was a strictly Na(+)-dependent and electrogenic transport process, mediated by a single transport component. 6. Investigation of the coupling ratio in luminal membrane vesicles suggested that 1 Na+:1 L-tryptophan are co-transported in the pars convoluta. In the pars recta, examination of the stoichiometry indicated that approx. 1 H+ and 2 Na+ (high affinity) or 1 Na+ (low affinity) are involved in the uptake of L-tryptophan.</jats:p> <scp>l</scp>-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney Biochemical Journal |
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1992 |
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Biochemical Journal |
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49 |
| title |
l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_unstemmed |
l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_full |
l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_fullStr |
l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_full_unstemmed |
l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_short |
l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_sort |
<scp>l</scp>-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1042/bj2860103 |
| publishDate |
1992 |
| physical |
103-110 |
| description |
<jats:p>1. The mechanism of the renal transport of L-tryptophan by basolateral and luminal membrane vesicles prepared from either the pars convoluta or the pars recta of the rabbit proximal tubule was studied. The uptake of L-tryptophan by basolateral membrane vesicles from the pars convoluta was found to be an Na(+)-dependent transport event. The Na(+)-conditional influx of the amino acid was stimulated in the presence of an inwardly directed H+ gradient. Lowering the pH without an H+ gradient had no effect, indicating that L-tryptophan is co-transported with H+. 3. On the other hand, no transient accumulation of L-tryptophan was observed in the presence or absence of Na+ in basolateral membrane vesicles from the pars recta. 4. In luminal membrane vesicles from the pars recta, the transient Na(+)-dependent accumulation of L-tryptophan occurred via a dual transport system. In addition, an inwardly directed H+ gradient could drive the uphill transport of L-tryptophan into these vesicles in both the presence and the absence of an Na+ gradient. 5. By contrast, the uptake of L-tryptophan by luminal membrane vesicles from the pars convoluta was a strictly Na(+)-dependent and electrogenic transport process, mediated by a single transport component. 6. Investigation of the coupling ratio in luminal membrane vesicles suggested that 1 Na+:1 L-tryptophan are co-transported in the pars convoluta. In the pars recta, examination of the stoichiometry indicated that approx. 1 H+ and 2 Na+ (high affinity) or 1 Na+ (low affinity) are involved in the uptake of L-tryptophan.</jats:p> |
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| description | <jats:p>1. The mechanism of the renal transport of L-tryptophan by basolateral and luminal membrane vesicles prepared from either the pars convoluta or the pars recta of the rabbit proximal tubule was studied. The uptake of L-tryptophan by basolateral membrane vesicles from the pars convoluta was found to be an Na(+)-dependent transport event. The Na(+)-conditional influx of the amino acid was stimulated in the presence of an inwardly directed H+ gradient. Lowering the pH without an H+ gradient had no effect, indicating that L-tryptophan is co-transported with H+. 3. On the other hand, no transient accumulation of L-tryptophan was observed in the presence or absence of Na+ in basolateral membrane vesicles from the pars recta. 4. In luminal membrane vesicles from the pars recta, the transient Na(+)-dependent accumulation of L-tryptophan occurred via a dual transport system. In addition, an inwardly directed H+ gradient could drive the uphill transport of L-tryptophan into these vesicles in both the presence and the absence of an Na+ gradient. 5. By contrast, the uptake of L-tryptophan by luminal membrane vesicles from the pars convoluta was a strictly Na(+)-dependent and electrogenic transport process, mediated by a single transport component. 6. Investigation of the coupling ratio in luminal membrane vesicles suggested that 1 Na+:1 L-tryptophan are co-transported in the pars convoluta. In the pars recta, examination of the stoichiometry indicated that approx. 1 H+ and 2 Na+ (high affinity) or 1 Na+ (low affinity) are involved in the uptake of L-tryptophan.</jats:p> |
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| spelling | Jessen, H Sheikh, M I 0264-6021 1470-8728 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1042/bj2860103 <jats:p>1. The mechanism of the renal transport of L-tryptophan by basolateral and luminal membrane vesicles prepared from either the pars convoluta or the pars recta of the rabbit proximal tubule was studied. The uptake of L-tryptophan by basolateral membrane vesicles from the pars convoluta was found to be an Na(+)-dependent transport event. The Na(+)-conditional influx of the amino acid was stimulated in the presence of an inwardly directed H+ gradient. Lowering the pH without an H+ gradient had no effect, indicating that L-tryptophan is co-transported with H+. 3. On the other hand, no transient accumulation of L-tryptophan was observed in the presence or absence of Na+ in basolateral membrane vesicles from the pars recta. 4. In luminal membrane vesicles from the pars recta, the transient Na(+)-dependent accumulation of L-tryptophan occurred via a dual transport system. In addition, an inwardly directed H+ gradient could drive the uphill transport of L-tryptophan into these vesicles in both the presence and the absence of an Na+ gradient. 5. By contrast, the uptake of L-tryptophan by luminal membrane vesicles from the pars convoluta was a strictly Na(+)-dependent and electrogenic transport process, mediated by a single transport component. 6. Investigation of the coupling ratio in luminal membrane vesicles suggested that 1 Na+:1 L-tryptophan are co-transported in the pars convoluta. In the pars recta, examination of the stoichiometry indicated that approx. 1 H+ and 2 Na+ (high affinity) or 1 Na+ (low affinity) are involved in the uptake of L-tryptophan.</jats:p> <scp>l</scp>-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney Biochemical Journal |
| spellingShingle | Jessen, H, Sheikh, M I, Biochemical Journal, l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney, Cell Biology, Molecular Biology, Biochemistry |
| title | l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_full | l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_fullStr | l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_full_unstemmed | l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_short | l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_sort | <scp>l</scp>-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| title_unstemmed | l-tryptophan uptake by segment-specific membrane vesicles from the proximal tubule of rabbit kidney |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1042/bj2860103 |