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Separation of two forms of rabbit metallothionein by isoelectric focusing
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Zeitschriftentitel: | Biochemical Journal |
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Personen und Körperschaften: | , , , |
In: | Biochemical Journal, 126, 1972, 3, S. 491-498 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Portland Press Ltd.
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Schlagwörter: |
author_facet |
Nordberg, G F Nordberg, M. Piscator, M. Vesterberg, O. Nordberg, G F Nordberg, M. Piscator, M. Vesterberg, O. |
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author |
Nordberg, G F Nordberg, M. Piscator, M. Vesterberg, O. |
spellingShingle |
Nordberg, G F Nordberg, M. Piscator, M. Vesterberg, O. Biochemical Journal Separation of two forms of rabbit metallothionein by isoelectric focusing General Medicine |
author_sort |
nordberg, g f |
spelling |
Nordberg, G F Nordberg, M. Piscator, M. Vesterberg, O. 0306-3283 Portland Press Ltd. General Medicine http://dx.doi.org/10.1042/bj1260491 <jats:p>Rabbits were given repeated injections of cadmium chloride. Cadmium- and zinc-containing protein fractions were obtained from the livers of these animals by precipitation procedures and Sephadex G-75 chromatography. The protein thus obtained showed several characteristics similar to those of the earlier described protein metallothionein. Further separation by isoelectric focusing showed two main protein peaks with isoelectric points at 3.9 and 4.5 respectively. Amino acid analysis of these two forms showed similar content of most amino acids [residues per cent.: cysteine (28%), aspartate (8%), threonine (5–6%), serine (12%), glycine (7%), alanine (13%), methionine (2%), isoleucine (2%)] but with a small difference in content of lysine (12 and 13% respectively), proline (9 and 5% respectively) and glutamate (2 and 4% respectively). The two forms of the protein both contained cadmium, but only the one with pI4.5 contained also significant amounts of zinc.</jats:p> Separation of two forms of rabbit metallothionein by isoelectric focusing Biochemical Journal |
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10.1042/bj1260491 |
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Portland Press Ltd., 1972 |
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Portland Press Ltd., 1972 |
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1972 |
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Portland Press Ltd. |
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Biochemical Journal |
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49 |
title |
Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_unstemmed |
Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_full |
Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_fullStr |
Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_full_unstemmed |
Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_short |
Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_sort |
separation of two forms of rabbit metallothionein by isoelectric focusing |
topic |
General Medicine |
url |
http://dx.doi.org/10.1042/bj1260491 |
publishDate |
1972 |
physical |
491-498 |
description |
<jats:p>Rabbits were given repeated injections of cadmium chloride. Cadmium- and zinc-containing protein fractions were obtained from the livers of these animals by precipitation procedures and Sephadex G-75 chromatography. The protein thus obtained showed several characteristics similar to those of the earlier described protein metallothionein. Further separation by isoelectric focusing showed two main protein peaks with isoelectric points at 3.9 and 4.5 respectively. Amino acid analysis of these two forms showed similar content of most amino acids [residues per cent.: cysteine (28%), aspartate (8%), threonine (5–6%), serine (12%), glycine (7%), alanine (13%), methionine (2%), isoleucine (2%)] but with a small difference in content of lysine (12 and 13% respectively), proline (9 and 5% respectively) and glutamate (2 and 4% respectively). The two forms of the protein both contained cadmium, but only the one with pI4.5 contained also significant amounts of zinc.</jats:p> |
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author | Nordberg, G F, Nordberg, M., Piscator, M., Vesterberg, O. |
author_facet | Nordberg, G F, Nordberg, M., Piscator, M., Vesterberg, O., Nordberg, G F, Nordberg, M., Piscator, M., Vesterberg, O. |
author_sort | nordberg, g f |
container_issue | 3 |
container_start_page | 491 |
container_title | Biochemical Journal |
container_volume | 126 |
description | <jats:p>Rabbits were given repeated injections of cadmium chloride. Cadmium- and zinc-containing protein fractions were obtained from the livers of these animals by precipitation procedures and Sephadex G-75 chromatography. The protein thus obtained showed several characteristics similar to those of the earlier described protein metallothionein. Further separation by isoelectric focusing showed two main protein peaks with isoelectric points at 3.9 and 4.5 respectively. Amino acid analysis of these two forms showed similar content of most amino acids [residues per cent.: cysteine (28%), aspartate (8%), threonine (5–6%), serine (12%), glycine (7%), alanine (13%), methionine (2%), isoleucine (2%)] but with a small difference in content of lysine (12 and 13% respectively), proline (9 and 5% respectively) and glutamate (2 and 4% respectively). The two forms of the protein both contained cadmium, but only the one with pI4.5 contained also significant amounts of zinc.</jats:p> |
doi_str_mv | 10.1042/bj1260491 |
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id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTA0Mi9iajEyNjA0OTE |
imprint | Portland Press Ltd., 1972 |
imprint_str_mv | Portland Press Ltd., 1972 |
institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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mega_collection | Portland Press Ltd. (CrossRef) |
physical | 491-498 |
publishDate | 1972 |
publishDateSort | 1972 |
publisher | Portland Press Ltd. |
record_format | ai |
recordtype | ai |
series | Biochemical Journal |
source_id | 49 |
spelling | Nordberg, G F Nordberg, M. Piscator, M. Vesterberg, O. 0306-3283 Portland Press Ltd. General Medicine http://dx.doi.org/10.1042/bj1260491 <jats:p>Rabbits were given repeated injections of cadmium chloride. Cadmium- and zinc-containing protein fractions were obtained from the livers of these animals by precipitation procedures and Sephadex G-75 chromatography. The protein thus obtained showed several characteristics similar to those of the earlier described protein metallothionein. Further separation by isoelectric focusing showed two main protein peaks with isoelectric points at 3.9 and 4.5 respectively. Amino acid analysis of these two forms showed similar content of most amino acids [residues per cent.: cysteine (28%), aspartate (8%), threonine (5–6%), serine (12%), glycine (7%), alanine (13%), methionine (2%), isoleucine (2%)] but with a small difference in content of lysine (12 and 13% respectively), proline (9 and 5% respectively) and glutamate (2 and 4% respectively). The two forms of the protein both contained cadmium, but only the one with pI4.5 contained also significant amounts of zinc.</jats:p> Separation of two forms of rabbit metallothionein by isoelectric focusing Biochemical Journal |
spellingShingle | Nordberg, G F, Nordberg, M., Piscator, M., Vesterberg, O., Biochemical Journal, Separation of two forms of rabbit metallothionein by isoelectric focusing, General Medicine |
title | Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_full | Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_fullStr | Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_full_unstemmed | Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_short | Separation of two forms of rabbit metallothionein by isoelectric focusing |
title_sort | separation of two forms of rabbit metallothionein by isoelectric focusing |
title_unstemmed | Separation of two forms of rabbit metallothionein by isoelectric focusing |
topic | General Medicine |
url | http://dx.doi.org/10.1042/bj1260491 |