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Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis
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| Zeitschriftentitel: | Biochemical Journal |
|---|---|
| Personen und Körperschaften: | , , |
| In: | Biochemical Journal, 143, 1974, 1, S. 67-72 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Portland Press Ltd.
|
| Schlagwörter: |
| author_facet |
Burgoyne, Leigh A. Hewish, Dean R. Mobbs, Jennifer Burgoyne, Leigh A. Hewish, Dean R. Mobbs, Jennifer |
|---|---|
| author |
Burgoyne, Leigh A. Hewish, Dean R. Mobbs, Jennifer |
| spellingShingle |
Burgoyne, Leigh A. Hewish, Dean R. Mobbs, Jennifer Biochemical Journal Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis Cell Biology Molecular Biology Biochemistry |
| author_sort |
burgoyne, leigh a. |
| spelling |
Burgoyne, Leigh A. Hewish, Dean R. Mobbs, Jennifer 0264-6021 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1042/bj1430067 <jats:p>The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca–Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000–63000 and the smallest double-stranded class has a molecular weight of approx. 120000–150000. Studies of the substructure of the DNA fragments produced by the Ca–Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.</jats:p> Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis Biochemical Journal |
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10.1042/bj1430067 |
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Biologie Chemie und Pharmazie |
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Portland Press Ltd., 1974 |
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Portland Press Ltd., 1974 |
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0264-6021 |
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1974 |
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Portland Press Ltd. |
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Biochemical Journal |
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49 |
| title |
Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_unstemmed |
Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_full |
Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_fullStr |
Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_full_unstemmed |
Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_short |
Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_sort |
mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1042/bj1430067 |
| publishDate |
1974 |
| physical |
67-72 |
| description |
<jats:p>The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca–Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000–63000 and the smallest double-stranded class has a molecular weight of approx. 120000–150000. Studies of the substructure of the DNA fragments produced by the Ca–Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.</jats:p> |
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| author | Burgoyne, Leigh A., Hewish, Dean R., Mobbs, Jennifer |
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| description | <jats:p>The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca–Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000–63000 and the smallest double-stranded class has a molecular weight of approx. 120000–150000. Studies of the substructure of the DNA fragments produced by the Ca–Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.</jats:p> |
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| imprint | Portland Press Ltd., 1974 |
| imprint_str_mv | Portland Press Ltd., 1974 |
| institution | DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| issn_str_mv | 0264-6021 |
| language | English |
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| mega_collection | Portland Press Ltd. (CrossRef) |
| physical | 67-72 |
| publishDate | 1974 |
| publishDateSort | 1974 |
| publisher | Portland Press Ltd. |
| record_format | ai |
| recordtype | ai |
| series | Biochemical Journal |
| source_id | 49 |
| spelling | Burgoyne, Leigh A. Hewish, Dean R. Mobbs, Jennifer 0264-6021 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1042/bj1430067 <jats:p>The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca–Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000–63000 and the smallest double-stranded class has a molecular weight of approx. 120000–150000. Studies of the substructure of the DNA fragments produced by the Ca–Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.</jats:p> Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis Biochemical Journal |
| spellingShingle | Burgoyne, Leigh A., Hewish, Dean R., Mobbs, Jennifer, Biochemical Journal, Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis, Cell Biology, Molecular Biology, Biochemistry |
| title | Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_full | Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_fullStr | Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_full_unstemmed | Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_short | Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_sort | mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| title_unstemmed | Mammalian chromatin substructure studies with the calcium–magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1042/bj1430067 |