SOLR
| _version_ |
1797365278018895872 |
| author |
Schmidt, Martin |
| author2 |
Hense, Sarah, Minoche, André E., Dohm, Juliane C., Himmelbauer, Heinz, Schmidt, Thomas, Zakrzewski, Falk |
| author2_role |
, , , , , |
| author2_variant |
s h sh, a e m ae aem, j c d jc jcd, h h hh, t s ts, f z fz |
| author_facet |
Schmidt, Martin, Hense, Sarah, Minoche, André E., Dohm, Juliane C., Himmelbauer, Heinz, Schmidt, Thomas, Zakrzewski, Falk |
| author_role |
|
| author_sort |
Schmidt, Martin |
| author_variant |
m s ms |
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Library A |
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sid-22-col-qucosa |
| container_reference |
143,2014,1-3 |
| contents |
DNA methylation is an essential epigenetic feature for the regulation and maintenance of heterochromatin. Satellite DNA is a repetitive sequence component that often occurs in large arrays in heterochromatin of subtelomeric, intercalary and centromeric regions. Knowledge about the methylation status of satellite DNA is important for understanding the role of repetitive DNA in heterochromatization. In this study, we investigated the cytosine methylation of the ancient satellite family pEV in the wild beet Beta procumbens. The pEV satellite is widespread in species-specific pEV subfamilies in the genus Beta and most likely originated before the radiation of the Betoideae and Chenopodioideae. In B. procumbens , the pEV subfamily occurs abundantly and spans intercalary and centromeric regions. To uncover its cytosine methylation, we performed chromosome-wide immunostaining and bisulfite sequencing of pEV satellite repeats. We found that CG and CHG sites are highly methylated while CHH sites show only low levels of methylation. As a consequence of the low frequency of CG and CHG sites and the preferential occurrence of most cytosines in the CHH motif in pEV monomers, this satellite family displays only low levels of total cytosine methylation. |
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610 |
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600 - Technology (Applied sciences) |
| dewey-ones |
610 - Medicine and health |
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610 |
| dewey-search |
610 |
| dewey-sort |
3610 |
| dewey-tens |
610 - Medicine and health |
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Online, Free |
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Technik, Medizin |
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medicine |
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ElectronicSerialComponentPart |
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Article, E-Article |
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Ebook |
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Article, E-Article |
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Article, E-Article |
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Buch |
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text-online-journal-child |
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Journal |
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Article, E-Article |
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ElectronicArticle |
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Article, E-Article |
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Article, E-Article |
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E-Article |
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not assigned |
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not assigned |
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143,2014,1-3 |
| id |
22-14-qucosa2-705761 |
| illustrated |
Not Illustrated |
| imprint |
Basel, Karger, 2014 |
| imprint_str_mv |
Online-Ausg.: 2020 |
| institution |
DE-105, DE-Gla1, DE-Brt1, DE-D161, DE-540, DE-Pl11, DE-Rs1, DE-Bn3, DE-Zi4, DE-Zwi2, DE-D117, DE-Mh31, DE-D275, DE-Ch1, DE-15, DE-D13, DE-L242, DE-L229, DE-L328 |
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English |
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2024-04-26T03:11:41.886Z |
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schmidt2014cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens |
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Qucosa |
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2014 |
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2014 |
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Basel |
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Karger |
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marcfinc |
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14-qucosa2-705761 |
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marcfinc |
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No subject assigned |
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22 |
| spelling |
Schmidt, Martin, Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens, Basel Karger 2014, Online-Ausg. 2020 Online-Ressource (Text) Technische Universität Dresden, DNA methylation is an essential epigenetic feature for the regulation and maintenance of heterochromatin. Satellite DNA is a repetitive sequence component that often occurs in large arrays in heterochromatin of subtelomeric, intercalary and centromeric regions. Knowledge about the methylation status of satellite DNA is important for understanding the role of repetitive DNA in heterochromatization. In this study, we investigated the cytosine methylation of the ancient satellite family pEV in the wild beet Beta procumbens. The pEV satellite is widespread in species-specific pEV subfamilies in the genus Beta and most likely originated before the radiation of the Betoideae and Chenopodioideae. In B. procumbens , the pEV subfamily occurs abundantly and spans intercalary and centromeric regions. To uncover its cytosine methylation, we performed chromosome-wide immunostaining and bisulfite sequencing of pEV satellite repeats. We found that CG and CHG sites are highly methylated while CHH sites show only low levels of methylation. As a consequence of the low frequency of CG and CHG sites and the preferential occurrence of most cytosines in the CHH motif in pEV monomers, this satellite family displays only low levels of total cytosine methylation., Beta-Procumben, Bisulfit-Sequenzierung, Dna-Methylierung, Immunfärbung, Satelliten-Dna, Zuckerrübe, Beta Procumbens, Bisulfite Sequencing, Dna Methylation, Immunostaining, Satellite Dna, Sugar Beet, Hense, Sarah, Minoche, André E., Dohm, Juliane C., Himmelbauer, Heinz, Schmidt, Thomas, Zakrzewski, Falk, 143,2014,1-3, text/html https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-705761 Online-Zugriff |
| spellingShingle |
Schmidt, Martin, Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens, DNA methylation is an essential epigenetic feature for the regulation and maintenance of heterochromatin. Satellite DNA is a repetitive sequence component that often occurs in large arrays in heterochromatin of subtelomeric, intercalary and centromeric regions. Knowledge about the methylation status of satellite DNA is important for understanding the role of repetitive DNA in heterochromatization. In this study, we investigated the cytosine methylation of the ancient satellite family pEV in the wild beet Beta procumbens. The pEV satellite is widespread in species-specific pEV subfamilies in the genus Beta and most likely originated before the radiation of the Betoideae and Chenopodioideae. In B. procumbens , the pEV subfamily occurs abundantly and spans intercalary and centromeric regions. To uncover its cytosine methylation, we performed chromosome-wide immunostaining and bisulfite sequencing of pEV satellite repeats. We found that CG and CHG sites are highly methylated while CHH sites show only low levels of methylation. As a consequence of the low frequency of CG and CHG sites and the preferential occurrence of most cytosines in the CHH motif in pEV monomers, this satellite family displays only low levels of total cytosine methylation., Beta-Procumben, Bisulfit-Sequenzierung, Dna-Methylierung, Immunfärbung, Satelliten-Dna, Zuckerrübe, Beta Procumbens, Bisulfite Sequencing, Dna Methylation, Immunostaining, Satellite Dna, Sugar Beet |
| title |
Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens |
| title_auth |
Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens |
| title_full |
Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens |
| title_fullStr |
Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens |
| title_full_unstemmed |
Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens |
| title_in_hierarchy |
|
| title_short |
Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens |
| title_sort |
cytosine methylation of an ancient satellite family in the wild beet beta procumbens |
| title_unstemmed |
Cytosine Methylation of an Ancient Satellite Family in the Wild Beet Beta procumbens |
| topic |
Beta-Procumben, Bisulfit-Sequenzierung, Dna-Methylierung, Immunfärbung, Satelliten-Dna, Zuckerrübe, Beta Procumbens, Bisulfite Sequencing, Dna Methylation, Immunostaining, Satellite Dna, Sugar Beet |
| topic_facet |
Beta-Procumben, Bisulfit-Sequenzierung, Dna-Methylierung, Immunfärbung, Satelliten-Dna, Zuckerrübe, Beta Procumbens, Bisulfite Sequencing, Dna Methylation, Immunostaining, Satellite Dna, Sugar Beet |
| url |
https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa2-705761 |
| urn |
urn:nbn:de:bsz:14-qucosa2-705761 |
| work_keys_str_mv |
AT schmidtmartin cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens, AT hensesarah cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens, AT minocheandree cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens, AT dohmjulianec cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens, AT himmelbauerheinz cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens, AT schmidtthomas cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens, AT zakrzewskifalk cytosinemethylationofanancientsatellitefamilyinthewildbeetbetaprocumbens |
