SOLR
| _version_ |
1797365297586372608 |
| author |
Gao, Wenling |
| author2 |
Rammelt, Stefan, Gelinsky, Michael |
| author2_role |
, |
| author2_variant |
s r sr, m g mg |
| author_facet |
Gao, Wenling, Rammelt, Stefan, Gelinsky, Michael |
| author_role |
|
| author_sort |
Gao, Wenling |
| author_variant |
w g wg |
| building |
Library A |
| collection |
sid-22-col-qucosa |
| contents |
I. Table of content II. List of abbreviations 1 Summary 2 Introduction 2.1 The process of bone healing 2.1.1 Stages of fracture healing 2.1.2 Early stage of inflammation 2.2 Clinical challenges 2.3 Microdialysis 2.3.1 The principle of Microdialysis 2.3.2 Parameters influencing the recovery 2.4 Aim of this study 3 Materials 3.1 Materials, devices and animals 3.2 Chemicals 3.3 Buffers and solutions 4 Methods 4.1 Background 4.2 In vitro microdialysis 4.2.1 Preparation of the protein solution 4.2.2 Microdialysis sampling procedure 4.3 In vivo microdialysis 4.3.1 Surgical procedure 4.3.2 Sample collection 4.4 Plasma samples 4.5 Determination of the fluid recovery 4.6 Determination of the relative recovery 4.7 Total protein measurement 4.8 Cytokine and growth factor analysis 4.8.1 IL-1β, IL-6, TNF-α and PDGF-BB ELISA 4.8.2 VEGF ELISA 4.8.3 TGF-β1 ELISA 4.8.4 BMP-2 ELISA 4.8.5 Proteome profilerTM array 4.9 Proteomic analysis 4.10 Histological analysis 4.11 Statistical analysis 5 Results 5.1 Protein selection 5.2 Determination of fluid recovery in vitro and in vivo 5.3 Determination of relative recovery (RR) in vitro 5.4 Determination of total protein concentration in vivo 5.5 Determination of cytokine and growth factor concentration in the microdialysate in vivo 5.5.1 IL-6 concentration 5.5.2 TGF-β1 concentration 5.5.3 IL-1β concentration 5.5.4 TNF-α concentration 5.5.5 PDGF-BB, BMP-2 and VEGF concentration 5.6 Determination of further cytokines and chemokines in the microdialysate in vivo 5.7 Protein determination using HPLC-MS/MS analysis 5.7.1 Proteins in the microdialysate 5.7.2 Proteins on the surface of the probe 5.8 Protein annotation 5.9 Determination of cytokines and growth factors in the blood plasma 5.9.1 Determination of IL-6 in the blood plasma 5.9.2 Determination of TGF-β1 in the blood plasma 5.9.3 Determination of PDGF-BB in the blood plasma 5.10 Histological analysis of the hematoma 6 Discussion 6.1 Fluid recovery 6.2 Influence of the crystalloid perfusate on relative recovery 6.3 Relative recovery of cytokines and growth factors in vitro 6.4 In vivo microdialysis 6.4.1 Total protein concentration 6.4.2 Annotation of proteins in hematoma identified by HPLC-MS/MS 6.4.3 Identification of cytokines and bone related proteins 6.5 The humoral inflammatory response 6.6 Cellular response 7 Conclusions 8 References 9 Appendix 9.1 Figure index 9.2 Table index III. Eidesstattliche Erklärung IV. Selbständigkeitserklärung V. Acknowledgements |
| dewey-full |
610 |
| dewey-hundreds |
600 - Technology (Applied sciences) |
| dewey-ones |
610 - Medicine and health |
| dewey-raw |
610 |
| dewey-search |
610 |
| dewey-sort |
3610 |
| dewey-tens |
610 - Medicine and health |
| facet_avail |
Online, Free |
| finc_class_facet |
Technik, Medizin |
| fincclass_txtF_mv |
medicine |
| format |
eBook, Thesis |
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Thesis |
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Thesis, Book, E-Book |
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Thesis, Book, E-Book |
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Buch, Buch |
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text-online-monograph-independent-thesis |
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e-Book |
| format_finc |
Book, E-Book, Thesis |
| format_legacy |
Thesis, Book |
| format_legacy_nrw |
Thesis, Book, E-Book |
| format_nrw |
Thesis, Book, E-Book |
| format_strict_txtF_mv |
E-Thesis |
| genre |
Hochschulschrift gnd-content |
| genre_facet |
Hochschulschrift |
| geogr_code |
not assigned |
| geogr_code_person |
not assigned |
| id |
22-14-qucosa-198476 |
| illustrated |
Not Illustrated |
| imprint |
Online-Ausg., 2016 |
| imprint_str_mv |
Online-Ausg.: 2016 |
| institution |
DE-105, DE-Gla1, DE-Brt1, DE-D161, DE-540, DE-Pl11, DE-Rs1, DE-Bn3, DE-Zi4, DE-Zwi2, DE-D117, DE-Mh31, DE-D275, DE-Ch1, DE-15, DE-D13, DE-L242, DE-L229, DE-L328 |
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|
| is_hierarchy_title |
|
| language |
English |
| last_indexed |
2024-04-26T03:11:59.372Z |
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gao2016monitoringthefirststagesoftheregenerationofbonedefects |
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Qucosa |
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2016 |
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marcfinc |
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14-qucosa-198476 |
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marcfinc |
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Ww 5544 |
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22 |
| spelling |
Gao, Wenling, Monitoring the first stages of the regeneration of bone defects, txt, nc, Online-Ausg. 2016 Online-Ressource (Text) Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, Dissertation Technische Universität Dresden 2013, I. Table of content II. List of abbreviations 1 Summary 2 Introduction 2.1 The process of bone healing 2.1.1 Stages of fracture healing 2.1.2 Early stage of inflammation 2.2 Clinical challenges 2.3 Microdialysis 2.3.1 The principle of Microdialysis 2.3.2 Parameters influencing the recovery 2.4 Aim of this study 3 Materials 3.1 Materials, devices and animals 3.2 Chemicals 3.3 Buffers and solutions 4 Methods 4.1 Background 4.2 In vitro microdialysis 4.2.1 Preparation of the protein solution 4.2.2 Microdialysis sampling procedure 4.3 In vivo microdialysis 4.3.1 Surgical procedure 4.3.2 Sample collection 4.4 Plasma samples 4.5 Determination of the fluid recovery 4.6 Determination of the relative recovery 4.7 Total protein measurement 4.8 Cytokine and growth factor analysis 4.8.1 IL-1β, IL-6, TNF-α and PDGF-BB ELISA 4.8.2 VEGF ELISA 4.8.3 TGF-β1 ELISA 4.8.4 BMP-2 ELISA 4.8.5 Proteome profilerTM array 4.9 Proteomic analysis 4.10 Histological analysis 4.11 Statistical analysis 5 Results 5.1 Protein selection 5.2 Determination of fluid recovery in vitro and in vivo 5.3 Determination of relative recovery (RR) in vitro 5.4 Determination of total protein concentration in vivo 5.5 Determination of cytokine and growth factor concentration in the microdialysate in vivo 5.5.1 IL-6 concentration 5.5.2 TGF-β1 concentration 5.5.3 IL-1β concentration 5.5.4 TNF-α concentration 5.5.5 PDGF-BB, BMP-2 and VEGF concentration 5.6 Determination of further cytokines and chemokines in the microdialysate in vivo 5.7 Protein determination using HPLC-MS/MS analysis 5.7.1 Proteins in the microdialysate 5.7.2 Proteins on the surface of the probe 5.8 Protein annotation 5.9 Determination of cytokines and growth factors in the blood plasma 5.9.1 Determination of IL-6 in the blood plasma 5.9.2 Determination of TGF-β1 in the blood plasma 5.9.3 Determination of PDGF-BB in the blood plasma 5.10 Histological analysis of the hematoma 6 Discussion 6.1 Fluid recovery 6.2 Influence of the crystalloid perfusate on relative recovery 6.3 Relative recovery of cytokines and growth factors in vitro 6.4 In vivo microdialysis 6.4.1 Total protein concentration 6.4.2 Annotation of proteins in hematoma identified by HPLC-MS/MS 6.4.3 Identification of cytokines and bone related proteins 6.5 The humoral inflammatory response 6.6 Cellular response 7 Conclusions 8 References 9 Appendix 9.1 Figure index 9.2 Table index III. Eidesstattliche Erklärung IV. Selbständigkeitserklärung V. Acknowledgements, Mikrodialyse, Knochen, Kritische Knochendefekte, Zytokine, Wachstumsfaktor, Probenentnahme, Microdialysis, Bone, Critical-Size Defect, Cytokine, Growth Factor, Sampling, Hochschulschrift gnd-content, Rammelt, Stefan, Gelinsky, Michael, text/html https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-198476 Online-Zugriff |
| spellingShingle |
Gao, Wenling, Monitoring the first stages of the regeneration of bone defects, I. Table of content II. List of abbreviations 1 Summary 2 Introduction 2.1 The process of bone healing 2.1.1 Stages of fracture healing 2.1.2 Early stage of inflammation 2.2 Clinical challenges 2.3 Microdialysis 2.3.1 The principle of Microdialysis 2.3.2 Parameters influencing the recovery 2.4 Aim of this study 3 Materials 3.1 Materials, devices and animals 3.2 Chemicals 3.3 Buffers and solutions 4 Methods 4.1 Background 4.2 In vitro microdialysis 4.2.1 Preparation of the protein solution 4.2.2 Microdialysis sampling procedure 4.3 In vivo microdialysis 4.3.1 Surgical procedure 4.3.2 Sample collection 4.4 Plasma samples 4.5 Determination of the fluid recovery 4.6 Determination of the relative recovery 4.7 Total protein measurement 4.8 Cytokine and growth factor analysis 4.8.1 IL-1β, IL-6, TNF-α and PDGF-BB ELISA 4.8.2 VEGF ELISA 4.8.3 TGF-β1 ELISA 4.8.4 BMP-2 ELISA 4.8.5 Proteome profilerTM array 4.9 Proteomic analysis 4.10 Histological analysis 4.11 Statistical analysis 5 Results 5.1 Protein selection 5.2 Determination of fluid recovery in vitro and in vivo 5.3 Determination of relative recovery (RR) in vitro 5.4 Determination of total protein concentration in vivo 5.5 Determination of cytokine and growth factor concentration in the microdialysate in vivo 5.5.1 IL-6 concentration 5.5.2 TGF-β1 concentration 5.5.3 IL-1β concentration 5.5.4 TNF-α concentration 5.5.5 PDGF-BB, BMP-2 and VEGF concentration 5.6 Determination of further cytokines and chemokines in the microdialysate in vivo 5.7 Protein determination using HPLC-MS/MS analysis 5.7.1 Proteins in the microdialysate 5.7.2 Proteins on the surface of the probe 5.8 Protein annotation 5.9 Determination of cytokines and growth factors in the blood plasma 5.9.1 Determination of IL-6 in the blood plasma 5.9.2 Determination of TGF-β1 in the blood plasma 5.9.3 Determination of PDGF-BB in the blood plasma 5.10 Histological analysis of the hematoma 6 Discussion 6.1 Fluid recovery 6.2 Influence of the crystalloid perfusate on relative recovery 6.3 Relative recovery of cytokines and growth factors in vitro 6.4 In vivo microdialysis 6.4.1 Total protein concentration 6.4.2 Annotation of proteins in hematoma identified by HPLC-MS/MS 6.4.3 Identification of cytokines and bone related proteins 6.5 The humoral inflammatory response 6.6 Cellular response 7 Conclusions 8 References 9 Appendix 9.1 Figure index 9.2 Table index III. Eidesstattliche Erklärung IV. Selbständigkeitserklärung V. Acknowledgements, Mikrodialyse, Knochen, Kritische Knochendefekte, Zytokine, Wachstumsfaktor, Probenentnahme, Microdialysis, Bone, Critical-Size Defect, Cytokine, Growth Factor, Sampling, Hochschulschrift |
| title |
Monitoring the first stages of the regeneration of bone defects |
| title_auth |
Monitoring the first stages of the regeneration of bone defects |
| title_full |
Monitoring the first stages of the regeneration of bone defects |
| title_fullStr |
Monitoring the first stages of the regeneration of bone defects |
| title_full_unstemmed |
Monitoring the first stages of the regeneration of bone defects |
| title_short |
Monitoring the first stages of the regeneration of bone defects |
| title_sort |
monitoring the first stages of the regeneration of bone defects |
| title_unstemmed |
Monitoring the first stages of the regeneration of bone defects |
| topic |
Mikrodialyse, Knochen, Kritische Knochendefekte, Zytokine, Wachstumsfaktor, Probenentnahme, Microdialysis, Bone, Critical-Size Defect, Cytokine, Growth Factor, Sampling, Hochschulschrift |
| topic_facet |
Mikrodialyse, Knochen, Kritische Knochendefekte, Zytokine, Wachstumsfaktor, Probenentnahme, Microdialysis, Bone, Critical-Size Defect, Cytokine, Growth Factor, Sampling, Hochschulschrift |
| url |
https://nbn-resolving.org/urn:nbn:de:bsz:14-qucosa-198476 |
| urn |
urn:nbn:de:bsz:14-qucosa-198476 |
| work_keys_str_mv |
AT gaowenling monitoringthefirststagesoftheregenerationofbonedefects, AT rammeltstefan monitoringthefirststagesoftheregenerationofbonedefects, AT gelinskymichael monitoringthefirststagesoftheregenerationofbonedefects |
